淨住 大慈 Daiji Kiyozumi

自己紹介 / Personal Data

淨住 大慈 Daiji Kiyozumi

名前
Name
淨住 大慈
Daiji Kiyozumi
誕生年
Year of Birth
1973
職名・学年
Job Title
特任助教
Assistant Professor
連絡先
Contact
kiyozumi(a)biken.osaka-u.ac.jp
TEL: +81-6-6879-4467
FAX: +81-6-6879-8376
大阪大学研究者総覧
Researcher's DB of Osaka University
Link
個人HP
Personal HP
ResearchGate

Google Scholar

趣味・特技 / What I Like

やりたいこと100個リストを作ってひとつずつ消化していくこと

To list up 100 things I want to do every year, and to do them one by one.

研究テーマ / Research Interests

生殖における細胞外環境の機能解明

Physiological function of extracellular environment in mammalian reproduction

キーワード / Keywords

細胞外環境、ルミクリン、細胞間相互作用、精子成熟
Extracellular environment, Lumicrine, Cell-cell communication, Sperm maturation

メッセージ / Messages

2014年10月から伊川研に異動しました。これまで器官形成における細胞外マトリックスの役割について研究してきたので、この経験を生かして細胞外環境という観点から生殖の分子機構解明に取り組んでいます。伊川研は個性的な学生×多様なバックグラウンドを持つスタッフ×ポジティブでユーモアに溢れたPIが力を合わせて大きなシナジーを生み出せるところが好きです。

I am studying in Ikawa's Lab since Oct. 2014. As I have been studying on the function of ECMs in organogenesis, in Ikawa's Lab I want to clarify the importance of extracellular environment in mammalian reproduction with my ECM research experiences. I like Ikawa's Lab because our lab can generate a great synergism with cooperation between lab members.

論文 / Publications

【原著論文】

  1. Kiyozumi, D., Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren KM, Horie M, Okabe M, Matzuk MM, Ikawa M. 2020. NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility. Science. 368, 1132-1135. doi: 10.1126/science.aay5134
  2. Kiyozumi, D., Nakano I, Sato-Nishiuchi R, Tanaka S, Sekiguchi K. 2020. Laminin is the ECM niche for trophoblast stem cells. Life Sci Alliance. 3, e201900515. doi: 10.26508/lsa.201900515
  3. Kiyozumi, D., Mori M, Kodani M, Ikawa M. 2020. Genetic mutation of Frem3 does not cause Fraser syndrome in mice. Exp Anim. 69, 104-109. doi: 10.1538/expanim.19-0088
  4. Abbasi F, Kodani M, Emori C, Kiyozumi, D., Mori M, Fujihara Y, Ikawa M. 2020. CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice. Cells. 9. 821. doi: 10.3390/cells9040821. 
  5. Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi, D., Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, Ikawa M. 2019. CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice. Biol Reprod. 101, 501-511. doi: 10.1093/biolre/ioz103
  6. Tobita T, Kiyozumi, D., Muto M, Noda T, Ikawa M. 2019. Lvrn expression is not critical for mouse placentation. J Reprod Dev. 65, 239-244. doi: 10.1262/jrd.2018-157
  7. Sato Y, Kiyozumi, D., Futaki S, Nakano I, Shimono C, Kaneko N, Ikawa M, Okabe M, Sawamoto K, Sekiguchi K. 2019. Ventricular-subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche. Mol Biol Cell. 30, 56-68. doi: 10.1091/mbc.E18-05-0286.
  8. Kiyozumi, D., Taniguchi, Y., Nakano, I., Toga, J., Yagi, E., Hasuwa, H., Ikawa, M., Sekiguchi, K., 2018. Laminin γ1 C-terminal Glu-to-Gln mutation induces early post-implantation lethality. Life Science Alliance, 1, e201800064, doi: 10.26508/lsa.201800064
  9. Muto, M., Fujihara, Y., Tobita, T., Kiyozumi, D., Ikawa, M., 2016. Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice. Biol. Reprod. 94, 6. doi:10.1095/biolreprod.115.133454
  10. Miyata, H., Castaneda, J.M., Fujihara, Y., Yu, Z., Archambeault, D.R., Isotani, A., Kiyozumi, D., Kriseman, M.L., Mashiko, D., Matsumura, T., Matzuk, R.M., Mori, M., Noda, T., Oji, A., Okabe, M., Prunskaite-Hyyrylainen, R., Ramirez-Solis, R., Satouh, Y., Zhang, Q., Ikawa, M., Matzuk, M.M., 2016. Genome engineering uncovers 54 evolutionarily conserved and testis-enriched genes that are not required for male fertility in mice. Proc. Natl. Acad. Sci. U. S. A. 113, 7704-10. doi:10.1073/pnas.1608458113
  11. Jeong, S.-J., Luo, R., Singer, K., Giera, S., Kreidberg, J., Kiyozumi, D., Shimono, C., Sekiguchi, K., Piao, X., 2013. GPR56 Functions Together with α3β1 Integrin in Regulating Cerebral Cortical Development. PLoS One 8, e68781. doi:10.1371/journal.pone.0068781
  12. Sato-Nishiuchi, R., Nakano, I., Ozawa, A., Sato, Y., Takeichi, M., Kiyozumi, D., Yamazaki, K., Yasunaga, T., Futaki, S., Sekiguchi, K., 2012. Polydom/SVEP1 is a ligand for integrin α9β1. J. Biol. Chem. 287, 25615-30. doi:10.1074/jbc.M112.355016
  13. Kiyozumi, D., Takeichi, M., Nakano, I., Sato, Y., Fukuda, T., Sekiguchi, K., 2012. Basement membrane assembly of the integrin α8β1 ligand nephronectin requires Fraser syndrome-associated proteins. J. Cell Biol. 197, 677-89. doi:10.1083/jcb.201203065
  14. Vissers, L.E.L.M., Cox, T.C., Maga, a M., Short, K.M., Wiradjaja, F., Janssen, I.M., Jehee, F., Bertola, D., Liu, J., Yagnik, G., Sekiguchi, K., Kiyozumi, D., van Bokhoven, H., Marcelis, C., Cunningham, M.L., Anderson, P.J., Boyadjiev, S. a, Passos-Bueno, M.R., Veltman, J. a, Smyth, I., Buckley, M.F., Roscioli, T., 2011. Heterozygous mutations of FREM1 are associated with an increased risk of isolated metopic craniosynostosis in humans and mice. PLoS Genet. 7, e1002278. doi:10.1371/journal.pgen.1002278
  15. Kiyozumi, D., Osada, A., Sugimoto, N., Weber, C.N., Ono, Y., Imai, T., Okada, A., Sekiguchi, K., 2011. Identification of genes expressed during hair follicle induction. J. Dermatol. 38, 674-9. doi:10.1111/j.1346-8138.2010.01050.x
  16. Kiyozumi, D., Nakano, I., Takahashi, K.L., Hojo, H., Aoyama, H., Sekiguchi, K., 2011. Fused pulmonary lobes is a rat model of human Fraser syndrome. Biochem. Biophys. Res. Commun. 411, 440-4. doi:10.1016/j.bbrc.2011.06.174
  17. Manabe, R. -i., Tsutsui, K., Yamada, T., Kimura, M., Nakano, I., Shimono, C., Sanzen, N., Furutani, Y., Fukuda, T., Oguri, Y., Shimamoto, K., Kiyozumi, D., Sato, Y., Sado, Y., Senoo, H., Yamashina, S., Fukuda, S., Kawai, J., Sugiura, N., Kimata, K., Hayashizaki, Y., Sekiguchi, K., 2008. Transcriptome-based systematic identification of extracellular matrix proteins. Proc. Natl. Acad. Sci. U. S. A. 105, 12849-12854. doi:10.1073/pnas.0803640105
  18. Kiyozumi, D., Sugimoto, N., Nakano, I., Sekiguchi, K., 2007. Frem3, a member of the 12 CSPG repeats-containing extracellular matrix protein family, is a basement membrane protein with tissue distribution patterns distinct from those of Fras1, Frem2, and QBRICK/Frem1. Matrix Biol. 26, 456-462.
  19. Kiyozumi, D., Sugimoto, N., Sekiguchi, K., 2006. Breakdown of the reciprocal stabilization of QBRICK/Frem1, Fras1, and Frem2 at the basement membrane provokes Fraser syndrome-like defects. Proc. Natl. Acad. Sci. U. S. A., 11981-11986.
  20. Kiyozumi, D., Osada, A., Sugimoto, N., Weber, C.N., Ono, Y., Imai, T., Okada, A., Sekiguchi, K., 2005. Identification of a novel cell-adhesive protein spatiotemporally expressed in the basement membrane of mouse developing hair follicle. Exp. Cell Res. 306, 9-23. doi:10.1016/j.yexcr.2005.01.020
  21. Osada, A., Kiyozumi, D., Tsutsui, K., Ono, Y., Weber, C.N., Sugimoto, N., Imai, T., Okada, A., Sekiguchi, K., 2005. Expression of MAEG, a novel basement membrane protein, in mouse hair follicle morphogenesis. Exp.Cell Res. 303, 148-159.
  22. Kiyozumi, D., Ishimizu, T., Nakanishi, T., Norioka, S., 2002. Pollen UDP-glucose pyrophosphorylase showing polymorphism well-correlated to the S genotype of Pyrus pyrifolia. Sex. Plant Reprod. 14, 315-323. doi:10.1007/s00497-001-0125-1
  23. Kiyozumi, D., Ishimizu, T., Nakanishi, T., Sakiyama, F., Norioka, S., 1999. Molecular Cloning and Nucleotide Sequencing of a cDNA Encoding UDP-Glucose Pyrophosphorylase of Japanese Pear (Accession No. AB013353). Plant Physiol. 119, 364.

【総説】

  1. 淨住大慈、伊川正人.  新規なシグナル伝達システム「ルミクライン」による雄性生殖路の機能と妊孕性の制御. 実験医学  第38巻18号 3132-3135. 2020年
  2. 淨住大慈、伊川正人. 精子が「一人前」になる仕組み – 「ルミクライン因子」が司る精子成熟の分子メカニズムに迫る. academist Journal 2020年10月15日 https://academist-cf.com/journal/?p=14679
  3. 淨住大慈. 精子が受精能をもつための鍵分子を発見! 化学 2020年8月号(75巻)page 75.
  4. Tobita, T., Kiyozumi, D., Ikawa, M., 2017. Placenta-specific gene manipulation using lentiviral vector and its application. Placenta 1-7. doi:10.1016/j.placenta.2017.09.012
  5. Kiyozumi, D., Sato-Nishiuchi, R., Sekiguchi, K., 2014. In situ detection of integrin ligands. Curr. Protoc. Cell Biol. 65, 10.19.1-10.19.17. doi:10.1002/0471143030.cb1019s65
  6. 淨住大慈、関口清俊. 基底膜のカスタマイゼーションとその器官形成における役割. THE LUNG perspectives 第15巻3号 336-340. 2007年
  7. 淨住大慈、関口清俊. 最適化された細胞外環境を提供する細胞外マトリックス. 実験医学 第23巻1号 52-57. 2005年