研究業績Achievement

研究業績

2022年～

  1. SLC35G3 is a UDP-N-acetylglucosamine transporter for sperm glycoprotein formation and underpins male fertility in mice
    Mashiko D, Tonai S, Miyata H, Matzuk MM, #Ikawa M.
    Elife. 2025 Nov 12:14:RP107494. doi: 10.7554/eLife.107494.
    https://pubmed.ncbi.nlm.nih.gov/40462929/

  2. Sperm and offspring production in a nonobstructive azoospermia mouse model via testicular mRNA delivery using lipid nanoparticles
    Mashiko D, Emori C, Hatanaka Y, Motooka D, Pan C, KanedaY, #Matzuk MM, #Ikawa  M ​​​​​​
    Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2516573122. doi: 10.1073/pnas.2516573122. Epub 2025 Oct 13.
    https://pubmed.ncbi.nlm.nih.gov/41082659/

  3. Formation of a complex between TMEM217 and the sodium-proton exchanger SLC9C1 is crucial for mouse sperm motility and male fertility
    Iida-Norita R, #Miyata H, Ninomiya A, Emori C, Kamoshita M, Pan C, Wang H, #Ikawa M ​​​​​
    Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2513924122. doi: 10.1073/pnas.2513924122. Epub 2025 Oct 15.
    https://pubmed.ncbi.nlm.nih.gov/41091759/

  4. GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding
    #*Noda T, *Uriu R, Mashiko D , Shinohara H,  Qu Y, Taira A, Matzuk RM, Tahala D, Nakano M, Araki K, Yu Z, Zhang Y, Matzuk MM, Ikawa M.
    Nat Commun. 2025 Sep 17;16(1):8264. doi: 10.1038/s41467-025-63805-4.
    https://pubmed.ncbi.nlm.nih.gov/40962834/

  5. *#Yokota S, *Hashimoto K, Sato T, Uemura K, Makiyama K, Nishimura T, Kitajima S, #Ogawa T.
    A long-term mouse testis organ culture system to identify germ cell damage induced by chemotherapy
    Curr Res Toxicol. 2025 Mar 11:8:100228. doi: 10.1016/j.crtox.2025.100228. eCollection 2025.
    https://pubmed.ncbi.nlm.nih.gov/40161557/

  6. Nakagawa T, Emori C, #Ikawa M.
    Trophectoderm-specific gene manipulation using adeno-associated viral vectors
    Exp Anim. 2025 Jan 11. doi: 10.1538/expanim.24-0165. Online ahead of print.
    https://pubmed.ncbi.nlm.nih.gov/39805595/

  7. Kamoshita M, Shirai H, Nakamura H, Kishimoto T, Hatanaka Y, Mashiko D, Esashika K, Yang J, Yamasaki S, Ogawa T, Kimura H & #Ikawa M.
    Development of the membrane ceiling method for in vitro spermatogenesis
    Sci Rep. 2025 Jan 3;15(1):625. doi: 10.1038/s41598-024-84965-1.
    https://pubmed.ncbi.nlm.nih.gov/39753886/

  8. #Deneke VE, Blaha A, Lu Y, Suwita JP, Draper JM, Phan CS, Panser K, Schleiffer A, Jacob L, Humer T, Stejskal K, Krssakova G, Roitinger E, Handler D, Kamoshita M, Vance TDR, Wang X, Surm JM, Moran Y, Lee JE, Ikawa M, #Pauli A.
    A conserved fertilization complex bridges sperm and egg in vertebrates
    Cell. 2024 Dec 12;187(25):7066-7078.e22. doi: 10.1016/j.cell.2024.09.035. Epub 2024 Oct 17.
    https://pubmed.ncbi.nlm.nih.gov/39423812/

  9. Ishikawa-Yamauchi Y, Emori C, Mori H, Endo T, Kobayashi K, Watanabe Y, Sagara H, Nagata T, Motooka D, Ninomiya A, Ozawa M, #Ikawa M.
    Age-associated aberrations of the cumulus-oocyte interaction and in the zona pellucida structure reduce fertility in female mice
    Commun Biol. 2024 Dec 24;7(1):1692. doi: 10.1038/s42003-024-07305-z.
    https://pubmed.ncbi.nlm.nih.gov/39719529/

  10. Ito Y,Kawauchi I,Yanagita Y,Sakai Y,Nishikawa M,Arakawa H,Kadoguchi M,Tamai I,Esashika K,Takahashi J,Ando O,Satoh T,Imai I,Adachi S,Chiba D,Arai K,Shiota K,Sawaguchi T,Yoshioka T,Fujimoto T, Yoshikuni T, Mizuno A, Aihara T, Sakura T,Kimura H&#Nakae H.
    Microphysiological systems for realizing microenvironment that mimics human physiology—functional material and its standardization applied to microfluidics
    SPRINGER NATURE. 2024/08/09 https://doi.org/10.1007/s42247-024-00822-x
    https://link.springer.com/article/10.1007/s42247-024-00822-x

  11. #Tokuoka Y, #Endo T, #Morikura T, #Hiradate Y, #Ikawa M,  #Funahashi A.
    Deep learning–based automated prediction of mouse seminiferous tubule stage by using bright-field microscopy
    biorxiv. 2024.08.07.doi: https://doi.org/10.1101/2024.08.07.606973
    https://www.biorxiv.org/content/10.1101/2024.08.07.606973v1

  12. Hashimoto K, Arakawa H,  Imamura R,  Nishimura T, Kitajima S, Sato T, Makiyama K,  #Ogawa T, #Yokota S.
    A novel alternative method for long-term evaluation of male reproductive toxicity and its recovery using a pre-pubertal mouse testis organ culture system
    J Appl Toxicol . 2024 May;44(5):784-793. doi: 10.1002/jat.4584. Epub 2024 Jan 23.
    https://pubmed.ncbi.nlm.nih.gov/38262615/

  13. #Ozawa M, Mori H, Endo T, Yamauchi-Ishikawa Y, Motooka D, Emori C, #Ikawa M.
    Age-related decline in spermatogenic activity accompanied by endothelial cell senescence in male mice.
    iScience. 2023 Nov 13;26(12):108456. doi: 10.1016/j.isci.2023.108456. eCollection 2023 Dec 15.
    https://pubmed.ncbi.nlm.nih.gov/38077127/
     
  14. Hashimoto K, Odaka H,Ishikawa-Yamauchi  Y, Nagata S,Nakamura H,Kimura H, Sato T, Makiyama K, #Ogawa T​.
    Culture-space control is effective in promoting haploid cell formation and spermiogenesis in vitro in neonatal mice.
    Sci Rep. 2023 Jul 31;13(1):12354. doi: 10.1038/s41598-023-39323-y.
    https://pubmed.ncbi.nlm.nih.gov/37524742/
     
  15. Matsumura T, Katagiri K, Yao T, Ishikawa-Yamauchi Y,Nagata S, Hashimoto K,Sato T, Kimura H, Shinohara T, Sanbo M, #Hirabayashi M, #Ogawa T.
    Generation of rat offspring using spermatids produced through in vitro spermatogenesis
    Sci Rep. 2023 Jul 26;13(1):12105. doi: 10.1038/s41598-023-39304-1.
    https://pubmed.ncbi.nlm.nih.gov/37495678/
     
  16. #Kiyozumi D, Shimada K, Chalick M, Emori C, Kodani M, Oura S, Noda T, Endo T,  Matzuk MM,  #Wreschner DH, #Ikawa M.
    A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility
    Nat Commun. 2023 Apr 24;14(1):2354. doi: 10.1038/s41467-023-37984-x.
    https://pubmed.ncbi.nlm.nih.gov/37095084/
     
  17. Feng X, Matsumura T, Yamashita Y, Sato T, Hashimoto K, Odaka H, Makino Y, Okada Y, Nakamura H, Kimura H, Fujii T ,#Ogawa T.
    In vitro spermatogenesis in isolated seminiferous tubules of immature mice
    PLoS One. 2023 Apr 6;18(4):e0283773. doi: 10.1371/journal.pone.0283773. eCollection 2023.
    https://pubmed.ncbi.nlm.nih.gov/37023052/
     
  18. Morohoshi A, #Miyata H, Tokuhiro K, Iida-Norita R, Noda T, Fujihara Y & #Ikawa M.
    Testis-enriched ferlin, FER1L5, is required for Ca2+-activated acrosome reaction and male fertility
    Sci Adv. 2023 Jan 25;9(4):eade7607. doi: 10.1126/sciadv.ade7607. Epub 2023 Jan 25.
    https://pubmed.ncbi.nlm.nih.gov/36696506/
     
  19. Oura S, Ninomiya A, Sugihara F, Matzuk MM & #Ikawa M.
    Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins.
    Sci Rep. 2022 Dec 23;12(1):22198. doi: 10.1038/s41598-022-26501-7.
    https://pubmed.ncbi.nlm.nih.gov/36564444/
     
  20. #Ozawa M, Taguchi J, Katsuma K, Ishikawa-Yamauchi Y, Kikuchi M, Sakamoto R, Yamada Y & #Ikawa M.
    Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice.
    Sci Rep. 2022 Dec 13;12(1):21558. doi: 10.1038/s41598-022-26107-z.
    https://pubmed.ncbi.nlm.nih.gov/36513736/
     
  21. Oura S, Mori H, #Ikawa M.
    Genome editing in mice and its application to the study of spermatogenesis.
    Gene and Genome Editing. 2022
    https://www.sciencedirect.com/science/article/pii/S2666388022000041
     
  22. #Ozawa M, *Emori C, #Ikawa M.
    CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models.
    J. Vis. Exp. 2022 (186), e64385, doi:10.3791/64385.
    https://www.jove.com/t/64385/crisprcas9-mediated-highly-efficient-gene-targeting-embryonic-stem
     
  23. Hirano K, Naomi Y, Nakamura Y, Sato T, Sato T, Ishiguro K, Ogawa T & #Yoshida S.
    Temperature sensitivity of DNA double-strand break repair underpins heat-induced meiotic failure in mouse spermatogenesis
    Commun Biol. 2022 May 26;5(1):504. doi: 10.1038/s42003-022-03449-y.
    https://www.jove.com/t/64385/crisprcas9-mediated-highly-efficient-gene-targeting-embryonic-stem
     
  24. #Kiyozumi D, #Ikawa M.
    Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies
    Front Endocrinol (Lausanne). 2022 May 4:13:876370. doi: 10.3389/fendo.2022.876370. eCollection 2022.
    https://pubmed.ncbi.nlm.nih.gov/35600599/