岡部 勝 Masaru Okabe

自己紹介 / Personal Data

岡部 勝 Masaru Okabe

名前
Name		 岡部 勝
Masaru Okabe
誕生年
Year of Birth		 1948
職名・学年
Job Title		 招聘研究員
invited researcher
連絡先
Contact		 okabe(a)biken.osaka-u.ac.jp
TEL: +81-6-6879-8374
FAX: +81-6-6879-8376
大阪大学研究者総覧
Researcher's DB of Osaka University		 大阪大学名誉教授
Professor Emeritus
個人HP
Personal HP		 My HP in Japanese

趣味・特技 / What I Like

テニス Tennis
ピアノ Piano
沈思黙考 Sit in silent thought

研究テーマ / Research Interests

  1. 受精のメカニズム
  2. 動物の形や大きさを決めるメカニズム
  1. Mechanism of fertilization
  2. Mechanism of forming the body shape and size

キーワード / Keywords

受精研究の歴史と仮説の変遷

メッセージ / Messages

  1. 受精卵は個体にとっての命の始まりであると言えます。ヒトは一度に2億とか3億もの精子を射精しますが、これに対して卵子は一つしか準備されません。マウスでも1千万の射精精子に対して、最大でも20個以下の卵子が排卵されるにすぎません。多くの精子の中から受精に適したものが選ばれているのか否か？受精の神秘に遺伝子改変動物を使ってせまりたいと思っています。
  2. 世界最小のほ乳類は人間の親指ほどの大きさしか有りません。それに対してシロナガスクジラの舌はアフリカ象ほどの大きさがあるそうです。マウスとラットという大きさの異なる動物でキメラを作り出せることがわかりました。この異種間キメラという秩序の中で「大きさ」という概念がどのように扱われるのかを研究したいと思っています。
  1. The fertilized egg can be recognized as the beginning of life for each individual. Humans ejaculate 2-3 x 10^8 spermatozoa against only one egg. In mouse, it is 1 x 10^7 against fewer than 20 eggs, at most. Is there a mechanism to select a single fertilizing spermatozoon among many? How do the sperm and egg meet and recognize each other? I would like to pursue the mystery of fertilization using gene-manipulated animals.
  2. The world's smallest mammal is the size of a human thumb, while the tongue of a blue whale is as large as an African elephant. We developed a method to make a mouse-rat chimera, even though these two creatures have different body sizes. I would like to elucidate the mechanism of body size determination using this xenogeneic chimera system.

論文 / Publications

  1. Okabe, M.
    Beware of memes in the interpretation of your results - lessons from gene‐disrupted mice in fertilization research
    FEBS Letters 22 May 2018: doi.org/10.1002/1873-3468.13101
  2. Okabe M.
    Sperm-egg interaction and fertilization: past, present, and future.
    Biol Reprod 2018; 99:134-146.
  3. Ishimoto K., Ikawa M. & Okabe, M.
    The mechanics clarifying counterclockwise rotation in most IVF eggs in mice.
    Scientific Reports3;7:43456. doi: 10.1038/srep43456 (2017).
  4. Okabe, M.
    The Acrosome Reaction: A Historical Perspective.
    Adv Anat Embryol Cell Biol 220, 1-13, doi:10.1007/978-3-319-30567-7_1 (2016).
  5. Muro, Y., Hasuwa, H., Isotani, A., Miyata, H., Yamagata, K., Ikawa, M., Yanagimachi, R., Okabe, M.
    Behavior of Mouse Spermatozoa in the Female Reproductive Tract from Soon after Mating to the Beginning of Fertilization.
    Biol Reprod 94, 80, doi:10.1095/biolreprod.115.135368 (2016).
  6. Isotani, A., Yamagata, K., Okabe, M. & Ikawa, M.
    Generation of Hprt-disrupted rat through mouse-rat ES chimeras.
    Scientific reports 6, 24215, doi:10.1038/srep24215 (2016).
  7. Okabe, M.
    Mechanisms of fertilization elucidated by gene-manipulated animals.
    Asian J Androl 17, 646-652, doi:10.4103/1008-682X.153299 (2015).
  8. Hasuwa, H., Ueda, J., Ikawa, M. & Okabe, M.
    MiR-200b and miR-429 Function in Mouse Ovulation and Are Essential for Female Fertility.
    Science 341, 71-73, doi:10.1126/science.1237999 (2013).
  9. Satouh, Y., Inoue, N., Ikawa, M. & Okabe, M.
    Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1.
    Journal of Cell Science 125, 4985-4990, doi:10.1242/jcs.100867 (2012).
  10. Isotani, A., Hatayama, H., Kaseda, K., Ikawa, M. & Okabe, M.
    Formation of a thymus from rat ES cells in xenogeneic nude mouse-rat ES chimeras.
    Genes to cells: devoted to molecular & cellular mechanisms 16, 397-405, doi:10.1111/j.1365-2443.2011.01495.x (2011).
  11. Inoue, N., Satouh, Y., Ikawa, M., Okabe, M. & Yanagimachi, R.
    Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs.
    Proceedings of the National Academy of Sciences of the United States of America108, 20008-20011, doi:10.1073/pnas.1116965108 (2011).
  12. Hasuwa, H., Muro, Y., Ikawa, M., Kato, N., Tsujimoto, Y., & Okabe, M
    Transgenic Mouse Sperm that Have Green Acrosome and Red Mitochondria Allow Visualization of Sperm and Their Acrosome Reaction in Vivo.
    Experimental Animals 59, 105-107 (2010).
  13. Inoue, N., Ikawa, M., Isotani, A. & Okabe, M.
    The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs.
    Nature 434, 234-238, doi:10.1038/nature03362 (2005).
  14. Nakanishi, T., Ikawa, M., Yamada, S., Toshimori, K. & Okabe, M.
    Alkalinization of acrosome measured by GFP as a pH indicator and its relation to sperm capacitation.
    Developmental Biology 237, 222-231, doi:10.1006/dbio.2001.0353 S0012-1606(01)90353-7 [pii] (2001).
  15. Miyado, K., Yamada, G., Yamada, S., Hasuwa, H., Nakamura, Y., Ryu, F., Suzuki, K., Kosai, K., Inoue, K., Ogura, A., Okabe, M. & Mekada, E.
    Requirement of CD9 on the egg plasma membrane for fertilization.
    Science287, 321-324, doi:10.1126/science.287.5451.321 (2000).
  16. Okabe, M., Ikawa, M., Kominami, K., Nakanishi, T. & Nishimune, Y.
    'Green mice' as a source of ubiquitous green cells.
    Febs Letters 407, 313-319, doi:10.1016/s0014-5793(97)00313-x (1997).
  17. Ikawa, M., Wada, I.,Kominami, K., Watanabe, D., Toshimori, K., Nishimune, Y. & Okabe, M.
    The putative chaperone calmegin is required for sperm fertility.
    Nature 387, 607-611 (1997).
  18. Ikawa, M., Kominami, K., Yoshimura, Y., Tanaka, K., Nishimune, Y. & Okabe, M.
    Green fluorescent protein as a marker in transgenic mice.
    Development Growth & Differentiation37, 455-459 (1995).
  19. Okabe, M., Yagasaki, M., Oda, H., Matzno, S., Kohama, Y., Mimura, T.
    Effect of a monoclonal anti-mouse sperm antibody (OBF13) on the interaction of mouse sperm with zona-free mouse and hamster eggs.
    Journal of reproductive immunology 13, 211-219, doi:10.1016/0165-0378(88)90002-2 (1988).

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