Sperm and offspring production in a nonobstructive azoospermia mouse model via testicular mRNA delivery using lipid nanoparticles
Mashiko D, Emori C, Hatanaka Y, Motooka D, Pan C, KanedaY, #Matzuk MM, #Ikawa M Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2516573122. doi: 10.1073/pnas.2516573122. Epub 2025 Oct 13. https://pubmed.ncbi.nlm.nih.gov/41082659/
Formation of a complex between TMEM217 and the sodium-proton exchanger SLC9C1 is crucial for mouse sperm motility and male fertility
Iida-Norita R, #Miyata H, Ninomiya A, Emori C, Kamoshita M, Pan C, Wang H, #Ikawa M Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2513924122. doi: 10.1073/pnas.2513924122. Epub 2025 Oct 15. https://pubmed.ncbi.nlm.nih.gov/41091759/
Maturation Status of Rat Lung Epithelial Cells in Interspecies Chimeras Generated by Blastocyst Complementation
Yamato Murata, Shunsuke Yuri, Masahito Ikawa, Ayako Isotani Genes Cells. 2025 Sep;30(5):e70046. doi: 10.1111/gtc.70046. https://pubmed.ncbi.nlm.nih.gov/40840556/
The transcription complex p52-ETS1 is essential for germinal center formation
Dhakshayini Morgan*, Biyan Zhan*, Kerem Fidan, Wenfai Pan, Thamil Selvan Vaiyapuri, Anandhkumar Raju, Dorcas Hei, Ting Yi See, Ita Novita Sari, Jun Wei Chan, Prativa Majee, Akhila Balachander, Jiabo Yu, Ada Hang-Heng Wong, Michelle Meng Huang Mok, Shi Hui Foo, Wei Lin Tang, Nicholas Ang, Ivan Tan, Yan Fen Peng, Patrick Jaynes, Shengli Xu, Gourisankar Ghosh, Shandy Shahabi, Anand D Jeyasekharan, Masahito Ikawa, Yongliang Zhang, Shanshan Wu Howland, Mai Chan Lau, Vivien Ya-Fan Wang, Kong-Peng Lam, Vinay Tergaonkar Nat Immunol. 2025 Sep;26(9):1553-1566. doi: 10.1038/s41590-025-02236-1. Epub 2025 Jul 25. https://pubmed.ncbi.nlm.nih.gov/40715659/
GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding #*Noda T, *Uriu R, Mashiko D , Shinohara H, Qu Y, Taira A, Matzuk RM, Tahala D, Nakano M, Araki K, Yu Z, Zhang Y, Matzuk MM, M Ikawa. Nat Commun. 2025 Sep 17;16(1):8264. doi: 10.1038/s41467-025-63805-4. https://pubmed.ncbi.nlm.nih.gov/40962834/
Proximity labeling of axonemal protein CFAP91 identifies EFCAB5 that regulates sperm motility
Wang H, Shimada K, Pham AH, Oyama Y, Kamoshita M, Kobayashi H, Oura S, Yabuta N, #Ikawa M, #Miyata H. Nat Commun. 2025 Sep 10;16(1):8238. doi: 10.1038/s41467-025-63705-7. https://pubmed.ncbi.nlm.nih.gov/40931011/
Testis expressed 50 is essential for maintaining sperm acrosome integrity during epididymal transit
Saori Haga, Kaori Nozawa, Ferheen Abbasi, Katarzyna Kent, Naoko Nagasawa, Tsutomu Endo, Haruhiko Miyata, Masahito Ikawa, Martin M Matzuk, Yoshitaka Fujihara Proc Natl Acad Sci U S A. 2025 Sep 9;122(36):e2507930122. doi: 10.1073/pnas.2507930122. Epub 2025 Sep 4. https://pubmed.ncbi.nlm.nih.gov/40906813/
Evolutionarily conserved role of telomerase reverse transcriptase in programming the microenvironment via regulation of the cGAS-STING pathway
Semih Can Akincilar,Kerem Fidan,Naveen Kumar,Qin Feng Ng,Prativa Majee,Lele Wu,Darryl J Y Han,Claire Hian Tzer Chan,Joelle Yi Heng Chua,Khaireen Idzham,Asami Oji,Wei Jie Jonathan Lee,Stefan H Oehlers,Masahito Ikawa,Vinay Tergaonkar Nat Cell Biol. 2025 Aug;27(8):1342-1356. doi: 10.1038/s41556-025-01706-w. Epub 2025 Aug 6. https://pubmed.ncbi.nlm.nih.gov/40770487/
An inducible model of human post-implantation development derived from primed and naive stem cells
Oura S, #Li L, #Wu J. Cell Stem Cell. 2025 Aug 25:S1934-5909(25)00297-8. doi: 10.1016/j.stem.2025.08.005. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/40885193/
Organization and Dynamics of Transcription Elongation Foci in Mouse Tissues
Chihiro Matsuda, Akane Ichiki, Yuko Sato, Yukino Kudo, Mika Saotome, Chihiro Takayama, Khoa Minh Le, Satoshi Uchino, Ryota Higuchi, Kazuhiko Kawata, Kosuke Tomimatsu, Manabu Ozawa, Masahito Ikawa, Yasuyuki Ohkawa, Yoshihiro Baba, Hiroshi Kimura J Mol Biol. 2025 Aug 13:169395. doi: 10.1016/j.jmb.2025.169395. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/40816716/
Rescue of male infertility by human PRSS55 in transgenic mice establishes a contraceptive research model
Courtney M Sutton, Kohei Umezu, Daisuke Mashiko, Masahito Ikawa, Irina V Larina, Thomas X Garcia, Martin M Matzuk Sci Rep. 2025 Aug 5;15(1):28657. doi: 10.1038/s41598-025-09604-9. https://pubmed.ncbi.nlm.nih.gov/40764777/
Compensation for X-linked Pdha1 silencing by Pdha2 is essential for meiotic double-strand break repair in spermatogenesis
Pan C, #Shimada K, Chang HY, Wang H, #Ikawa M Development. 2025 Jul 18:dev.204683. doi: 10.1242/dev.204683. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/40679056/
An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling
#Hiraoka T, Aikawa S, Mashiko D, Nakagawa T, Shirai H, Hirota Y, Kimura H & #Ikawa M. Nat Commun. 2025 Jul 1;16(1):5755. doi: 10.1038/s41467-025-60610-x. https://pubmed.ncbi.nlm.nih.gov/40595542/
Deep-learning-based automated prediction of mouse seminiferous tubule stage by using bright-field microscopy
Tokuoka Y, Endo T, Morikura T, Hiradate Y, #Ikawa M, #Funahashi A. Sci Rep. 2025 Jul 1;15(1):21849. doi: 10.1038/s41598-025-06727-x. https://pubmed.ncbi.nlm.nih.gov/40594622/
CRISPR/Cas9-mediated genome editing reveals seven testis-enriched transmembrane glycoproteins dispensable for male fertility in mice
Ogawa Y, #Lu Y, Kiyozumi D, Chang HY, #Ikawa M. Andrology. 2025 Jul;13(5):1251-1260. doi: 10.1111/andr.13564. Epub 2023 Dec 12. https://pubmed.ncbi.nlm.nih.gov/38084666/
Deep learning-based automated prediction of mouse seminiferous tubule stage by using bright-field microscopy
Yuta Tokuoka,Tsutomu Endo,Takashi Morikura,Yuki Hiradate,Masahito Ikawa,Akira Funahashi Sci Rep. 2025 Jul 1;15(1):21849. doi: 10.1038/s41598-025-06727-x. https://pubmed.ncbi.nlm.nih.gov/40594622/
Mouse genome engineering uncovers 18 genes dispensable for male reproduction
Chang H, #Lu Y, Yamamoto K, Sun J, Shimada K, Hiradate Y, Fujihara Y, #Ikawa M Andrology. 2025 Jun 26. doi: 10.1111/andr.70088. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/40572022/
Generation of a Mouse Model of Fuchs Endothelial Corneal Dystrophy by Knock-in of CTG Trinucleotide Repeat Expansion in the TCF4 Gene
Oyama Y, Ito S, Yuasa T, Ueda M, Chiba S, Nakagawa T, Izumi A, #Ikawa M, Koizumi N, #Okumura N. Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):18. doi: 10.1167/iovs.66.6.18. https://pubmed.ncbi.nlm.nih.gov/40465262/
KIF2C Deletion Causes Meiotic Abnormalities and Nonobstructive Azoospermia in Mice
Kitakaze H, #Miyata H, Oyama Y, Pan C, Kujime Y, Tsujimura G, Imanaka T, Kuribayashi S, Ueda N, Takezawa K, Fukuhara S, Nonomura N, #Ikawa M. Reprod Med Biol. 2025 May 27;24(1):e12659. doi: 10.1002/rmb2.12659. eCollection 2025 Jan-Dec. https://pubmed.ncbi.nlm.nih.gov/40433108/
SLC35G3 is a UDP-N-acetylglucosamine transporter for sperm glycoprotein formation and underpins male fertility in mice
Mashiko D, Tonai S, Miyata H, Matzuk MM, #Ikawa M. bioRxiv[Preprint]. 2025 May 15:2025.05.12.653469. doi: 10.1101/2025.05.12.653469. https://pubmed.ncbi.nlm.nih.gov/40462929/
ROLE OF INTRATESTICULAR CREATINE IN REGULATING THE BLOOD-TESTIS BARRIER AND ITS IMPACT ON SPERMATOGENESIS
Sohei Kuribayashi,Shinichiro Fukuhara,Hiroaki Kitakaze,Go Tsujimura,Takahiro Imanaka,Norichika Ueda,Kentaro Takezawa,Scott Lundy,Masahito Ikawa,Norio Nonomura JOURNAL OF UROLOGY. Volume 213.Issue 5S. May 2025. Page: e171. doi: 10.1097/01.JU.0001109764.27496.08.06
Zinc is a Key Regulator of the Sperm-Specific K+ Channel (Slo3) Function
Andriani RT, Pipatpolkai T, Miyata H, Ikawa M, Okamura Y, #Kawai T. eLife https://doi.org/10.7554/eLife.105450.1 https://elifesciences.org/reviewed-preprints/105450v1
The absence of both RIBC1 and RIBC2 induces decreased sperm motility and litter size in male mice #Katsuma K, Shimada K, Tonai S, Mashiko D, Iida-Norita R, Kaneda Y, Miyata H, #Ikawa M. Andrology. 2025 Apr 23. doi: 10.1111/andr.70045. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/40265983/
Heterozygous Tcf4 Deficiency Mitigates Fuchs Endothelial Corneal Dystrophy Progression in a Mouse Model
*Ito S, *Oyama Y, Yuasa T, Amano K, Onishi K, Izumi A, Jun AS, Ikawa M, Koizumi N, #Okumura N. Invest Ophthalmol Vis Sci. 2025 Apr 1;66(4):19. doi: 10.1167/iovs.66.4.19. https://pubmed.ncbi.nlm.nih.gov/40197778/
Therapeutic Potential of Emricasan, a Pan-Caspase Inhibitor, in Reducing Cell Death and Extracellular Matrix Accumulation in Fuchs Endothelial Corneal Dystrophy.
Fujimoto S, Endo M, Tonomura S, Tsuji F, Haraguchi H, Hasegawa K, Numao T, Izumi A, Tourtas T, Schlötzer-Schrehardt U, Kruse F, Oyama Y, Ikawa M, Jun AS, Koizumi N, #Okumura N. Cells. 2025 Mar 27;14(7):498. doi: 10.3390/cells14070498. https://pubmed.ncbi.nlm.nih.gov/40214452/
Retraction for Hiramatsu et al., "The Mechanism of Pertussis Cough Revealed by the Mouse-Coughing Model"
Hiramatsu Y, Suzuki K, Nishida T, Onoda N, Satoh T, Akira S, Ikawa M, Ikeda H, Kamei J, Derouiche S, Tominaga M, #Horiguchi Y. mBio. 2025 Mar 12;16(3):e0392124. doi: 10.1128/mbio.03921-24. Epub 2025 Feb 5. https://pubmed.ncbi.nlm.nih.gov/39907486/
Allosteric inhibition of the IZUMO1-JUNO fertilization complex by the naturally occurring antisperm antibody OBF13.
Lu Y, Ikawa M, #Tang S. Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2425952122. doi: 10.1073/pnas.2425952122. Epub 2025 Mar 5. https://pubmed.ncbi.nlm.nih.gov/40042902/
TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice
*Kaneda Y, #*Lu Y, *Sun J, Shimada K, Emori C, Noda T, Koyano T, Matsuyama M, #Miyata H, #Ikawa M. Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2417943122. doi: 10.1073/pnas.2417943122. Epub 2025 Mar 3. https://pubmed.ncbi.nlm.nih.gov/40030029/
Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms.
Okutani MS, Okamura S, Gis T, Sasaki H, Lee S, Kashiwabara A, Goto S, Matsumoto M, Yamawaki M, Miyazaki T, Nakagawa T, Ikawa M, Kamitani W, Takekawa S, Yamanishi K, #Ebina H. Elife. 2025 Feb 11:13:RP97532. doi: 10.7554/eLife.97532. https://pubmed.ncbi.nlm.nih.gov/39932490/
Ccer1 is a spermatid-specific gene required for spermatogenesis and male fertility.
Sammer B, Schmid P, Miyata H, Kazi S, Honkimaa AL, Petrov P, Kapiainen E, Miinalainen l, Izzi V, Ikawa M, #Prunskaite-Hyyryläinen R. Int J Dev Biol. 2024;68(4):251-262. doi: 10.1387/ijdb.240205rp. https://pubmed.ncbi.nlm.nih.gov/39868420/
Loss of CCDC188 causes male infertility with defects in the sperm head-neck connection in mice†
Qiu Y, #Shimada K, Yamamoto K, #Ikawa M. Biol Reprod. 2025 Jan 14;112(1):169-178. doi: 10.1093/biolre/ioae137. https://pubmed.ncbi.nlm.nih.gov/39292630/
Trophectoderm-specific gene manipulation using adeno-associated viral vectors
Nakagawa T, Emori C,＃Ikawa M. Exp Anim. 2025 Jan 11. doi: 10.1538/expanim.24-0165. Online ahead of print. https://pubmed.ncbi.nlm.nih.gov/39805595/
Development of the membrane ceiling method for in vitro spermatogenesis
Kamoshita M, Shirai H, Nakamura H, Kishimoto T, Hatanaka Y, Mashiko D, Esashika K, Yang J, Yamasaki S, Ogawa T, #Kimura H, #Ikawa M. Sci Rep. 2025 Jan 3;15(1):625. doi: 10.1038/s41598-024-84965-1. https://pubmed.ncbi.nlm.nih.gov/39753886