*equal contribution #corresponding author
1）査読付き欧文国際雑誌に掲載された原著論文
*Ueki H, *Tomita Y, *Duong C, Mitake H, Kiso M, Furusawa Y, Zhao D, da Silva Lopes TJ, Wu L, Feng H, Yamayoshi S, Fukuyama S, Yamashita M, Ozawa M, Ikawa M, Yoshida N, Watanabe T, #Kawaoka Y. A CRISPR knockout mouse library for functional genomics in influenza research. Cell. 2026 Jun 16. pii: S0092-8674(26)00591-X. doi: 10.1016/j.cell.2026.05.032. PMID: 42302780.
Takemoto K, Kent K, Nozawa K, Tonai S, Pham AH, Fujimoto T, Garcia TX, Matzuk MM, #Ikawa M, #Tokuhiro K. Functional analysis of genes enriched in male reproductive organs: validation via fertility assessment of 14 knockout mouse lines. Front Cell Dev Biol. 2026 May 28;14:1851315. doi: 10.3389/fcell.2026.1851315. eCollection 2026. PMID: 42293750. PMC13253961.
*Nakano M, *Yamaga K, Uriu R, Taira A, Masuda K, Araki K, Isotani A, Yuri S, Takeo T, #Ikawa M, #Noda T. Rat DCST1 and DCST2 are essential for sperm-egg fusiondagger. Biol Reprod. 2026 May 23. pii: 8691489. doi: 10.1093/biolre/ioag111. PMID: 42176245.
Stein P, Emori C, Padilla-Banks E, Radonova L, Gruzdev A, Ikawa M, #Williams CJ. Beware of your "oocyte specific" Cre line: somatic cell Cre expression in several Zp3-Cre lines and the Gdf9-iCre transgenic linedagger. Biol Reprod. 2026 Apr 30. pii: 8665608. doi: 10.1093/biolre/ioag093. PMID: 42059582.
Kaneda Y, #Miyata H, #Ikawa M. Testis-Enriched F-Box Protein FBXO39 Is Important for Spermiogenesis and Male Fertility in Mice. Andrology. 2026 Mar 31. doi: 10.1111/andr.70225. PMID: 41916926.
Liao Z, Zhang Q, Shimada K, Nozawa K, Tang S, Ikawa M, Monsivais D, #Matzuk MM. Versatile SMAD2 and SMAD3 epitope-tagged mouse models for genomic profiling of TGFbeta signaling: Uncovering GDF9-SMAD2/3 targets. Proc Natl Acad Sci U S A. 2026 Apr 7;123(14):e2600071123. doi: 10.1073/pnas.2600071123. Epub 2026 Mar 30. PMID: 41911462. PMC13056123.
Nagata S, Ishikawa-Yamauchi Y, Katagiri K, Sato T, Ikawa M, #Ogawa T. Optimization of oxygen concentration and glycosphingolipid effects on spermatogenesis in mouse testicular culturedagger. Biol Reprod. 2026 Mar 16;114(3):1079-1090. doi: 10.1093/biolre/ioaf284. PMID: 41432244.
Emori C, Tanaka H, Nakagawa T, Akita H, #Ikawa M. Lipid nanoparticle-mediated mRNA delivery system into preimplantation embryosdagger. Biol Reprod. 2026 Mar 16;114(3):1070-1078. doi: 10.1093/biolre/ioaf262. PMID: 41307534.
Mashiko D, Tonai S, #Ikawa M. ADAM5 is required for sperm-zona pellucida binding and sperm oviduct migrationdagger. Biol Reprod. 2026 Mar 16;114(3):1091-1100. doi: 10.1093/biolre/ioaf254. PMID: 41263505.
Aikawa S, Shimizu-Hirota R, Sakashita A, He X, Hiratsuka D, Ishizawa C, Iida R, Fukui Y, Hiraoka T, Matsuo M, Takeda N, Ikawa M, Osuga Y, #Hirota Y. HIF2alpha-induced lysyl oxidase safeguards successful pregnancy by remodelling collagens at the feto-maternal interface. Cell Death Dis. 2026 Mar 9;17(1). pii: 10.1038/s41419-026-08485-8. doi: 10.1038/s41419-026-08485-8. PMID: 41803091. PMC12972092.
Horike N, Oura S, Koyamatsu S, Tanaka N, Iimori Y, Fujita K, Nemoto T, Ikawa M, #Tsumaki N. Excess FGFR3 signaling in achondroplasia disrupts turnover of resting zone chondrocytes via CREB signaling. Nat Commun. 2026 Feb 26;17(1). pii: 10.1038/s41467-026-69507-9. doi: 10.1038/s41467-026-69507-9. PMID: 41748604. PMC12946275.
#Ozawa M, Taguchi J, Mori H, Kikuchi M, Muratani M, Yamamoto T, Yamada Y, Yoshida N, Ikawa M. PTBP1 supports mouse spermatogenesis by facilitating cytoskeletal organization through the mTORC2-PKCalpha pathway in Sertoli cells. Commun Biol. 2026 Jan 29;9(1). pii: 10.1038/s42003-026-09636-5. doi: 10.1038/s42003-026-09636-5. PMID: 41612039. PMC12957323.
Taguchi J, Kikuchi M, Jeon H, Shimizu R, Mori H, Ikawa M, Yamada Y, Sato K, Ikeda T, Yamazaki S, #Ozawa M. A scalable two-step genome editing strategy for generating full-length gene-humanized mice at diverse genomic loci. Nat Commun. 2026 Jan 14;17(1):356. doi: 10.1038/s41467-025-67900-4. PMID: 41535270. PMC12804900.
#Suzuki N, Suzuki A, Tamura S, Tokumaru S, Notoh H, Takagi A, Suzuki S, Isotani A, Suzuki N, Okamoto S, Kanematsu T, Ikawa M, Hayakawa F, Kiyoi H, Kojima T, Matsushita T. Antithrombotic Therapy on Antithrombin Resistance in a Mouse Model. Arterioscler Thromb Vasc Biol. 2026 Mar;46(3):e324007. doi: 10.1161/ATVBAHA.125.324007. Epub 2026 Jan 8. PMID: 41503699. PMC12931876.
Okamura Y, Nakanishi K, Inada R, Maeda R, Maeshima K, Mizutani M, Ohashi W, Ikawa M, Sugiura Y, Takeda K, #Umemoto E. GPR35 mediates monocyte recruitment to the sites of Listeria monocytogenes infection. Cell Mol Life Sci. 2025 Dec 2;82(1):433. doi: 10.1007/s00018-025-05912-5. PMID: 41329343. PMC12673015.
Wang H, Pham AH, Luo M, #Ikawa M, #Miyata H. Microtubule Inner Protein CFAP77 Contributes to Sperm Motility and Male Fertility in Mice. Andrology. 2026 Jul;14(5):1240-1248. doi: 10.1111/andr.70152. Epub 2025 Nov 29. PMID: 41316927. PMC13002332.
Mashiko D, Tonai S, Miyata H, Matzuk MM, #Ikawa M. SLC35G3 is a UDP-N-acetylglucosamine transporter for sperm glycoprotein formation and underpins male fertility in mice. Elife. 2025 Nov 12;14. pii: 107494. doi: 10.7554/eLife.107494. PMID: 41222000. PMC12611266.
Qiu Y, #Shimada K, #Ikawa M. CRISPR/Cas9-mediated Genome-editing Reveals 10 Testis-enriched Genes and One Non-testis-enriched Gene are Dispensable for Male Fecundity in Mice. Andrology. 2025 Nov 10. doi: 10.1111/andr.70144. PMID: 41208519. PMC12961275.
Komuro M, Mitsui Y, Edamoto M, Watanabe J, Nagashima Y, Naito H, Ikawa M, #Satoh T. Loss of Actrt1 in non-hematopoietic stroma suppresses pathological angiogenesis and tumor progression. Biochem Biophys Res Commun. 2025 Nov 19;790:152916. doi: 10.1016/j.bbrc.2025.152916. Epub 2025 Nov 4. PMID: 41197408.
Ogonuki N, Hino T, Fujiwara Y, Osawa Y, Mizuno S, Sugiyama F, Kunieda T, Otsuki J, Oura S, Furuse T, Okada Y, Tamura M, de la Casa-Esperon E, Ikawa M, Inoue K, #Ogura A. Spermatocyte injection into meiotic oocytes rescues diplotene, but not pachytene, arrest in azoospermic mutant mice. Hum Reprod Open. 2025 Oct 22;2025(4):hoaf067. doi: 10.1093/hropen/hoaf067. eCollection 2025. PMID: 41268068. PMC12628785.
Iida-Norita R, #Miyata H, Ninomiya A, Emori C, Kamoshita M, Pan C, Wang H, #Ikawa M. Formation of a complex between TMEM217 and the sodium-proton exchanger SLC9C1 is crucial for mouse sperm motility and male fertility. Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2513924122. doi: 10.1073/pnas.2513924122. Epub 2025 Oct 15. PMID: 41091759. PMC12557800.
Mashiko D, Emori C, Hatanaka Y, Motooka D, Pan C, Kaneda Y, #Matzuk MM, #Ikawa M. Sperm and offspring production in a nonobstructive azoospermia mouse model via testicular mRNA delivery using lipid nanoparticles. Proc Natl Acad Sci U S A. 2025 Oct 21;122(42):e2516573122. doi: 10.1073/pnas.2516573122. Epub 2025 Oct 13. PMID: 41082659. PMC12557808.
*#Noda T, *Uriu R, Mashiko D, Shinohara H, Qu Y, Taira A, Matzuk RM, Tahala D, Nakano M, Araki K, Yu Z, Zhang Y, #Matzuk MM, #Ikawa M. GALNTL5 binds GalNAc and is required for migration through the uterotubal junction and sperm-zona pellucida binding. Nat Commun. 2025 Sep 17;16(1):8264. doi: 10.1038/s41467-025-63805-4. PMID: 40962834. PMC12443989.
Wang H, Shimada K, Pham AH, Oyama Y, Kamoshita M, Kobayashi H, Oura S, Yabuta N, #Ikawa M, #Miyata H. Proximity labeling of axonemal protein CFAP91 identifies EFCAB5 that regulates sperm motility. Nat Commun. 2025 Sep 10;16(1):8238. doi: 10.1038/s41467-025-63705-7. PMID: 40931011. PMC12423330.
Haga S, Nozawa K, Abbasi F, Kent K, Nagasawa N, Endo T, Miyata H, Ikawa M, Matzuk MM, #Fujihara Y. Testis expressed 50 is essential for maintaining sperm acrosome integrity during epididymal transit. Proc Natl Acad Sci U S A. 2025 Sep 9;122(36):e2507930122. doi: 10.1073/pnas.2507930122. Epub 2025 Sep 4. PMID: 40906813. PMC12435281.
Murata Y, Yuri S, Ikawa M, #Isotani A. Maturation Status of Rat Lung Epithelial Cells in Interspecies Chimeras Generated by Blastocyst Complementation. Genes Cells. 2025 Sep;30(5):e70046. doi: 10.1111/gtc.70046. PMID: 40840556.
Matsuda C, Ichiki A, Sato Y, Kudo Y, Saotome M, Takayama C, Le KM, Uchino S, Higuchi R, Kawata K, Tomimatsu K, Ozawa M, Ikawa M, Ohkawa Y, Baba Y, #Kimura H. Organization and Dynamics of Transcription Elongation Foci in Mouse Tissues. J Mol Biol. 2026 Jan 1;438(1):169395. doi: 10.1016/j.jmb.2025.169395. Epub 2025 Aug 13. PMID: 40816716.
Pan C, #Shimada K, Chang HY, Wang H, #Ikawa M. Compensation for X-linked Pdha1 silencing by Pdha2 is essential for meiotic double-strand break repair in spermatogenesis. Development. 2025 Aug 1;152(15). pii: 368830. doi: 10.1242/dev.204683. Epub 2025 Aug 7. PMID: 40679056. PMC12377818.
*Akincilar SC, *Fidan K, Kumar N, Ng QF, Majee P, Wu L, Han DJY, Chan CHT, Chua JYH, Idzham K, Oji A, Lee WJJ, Oehlers SH, Ikawa M, #Tergaonkar V. Evolutionarily conserved role of telomerase reverse transcriptase in programming the microenvironment via regulation of the cGAS-STING pathway. Nat Cell Biol. 2025 Aug;27(8):1342-1356. doi: 10.1038/s41556-025-01706-w. Epub 2025 Aug 6. PMID: 40770487.
Sutton CM, Umezu K, Mashiko D, Ikawa M, Larina IV, Garcia TX, #Matzuk MM. Rescue of male infertility by human PRSS55 in transgenic mice establishes a contraceptive research model. Sci Rep. 2025 Aug 5;15(1):28657. doi: 10.1038/s41598-025-09604-9. PMID: 40764777. PMC12325665.
*Morgan D, *Zhang B, Fidan K, Pan W, Vaiyapuri TS, Raju A, Hei D, See TY, Sari IN, Chan JW, Majee P, Balachander A, Yu J, Wong AH, Mok MMH, Foo SH, Tang WL, Ang N, Tan I, Peng YF, Jaynes P, Xu S, Ghosh G, Shahabi S, Jeyasekharan AD, Ikawa M, Zhang Y, Howland SW, Lau MC, Wang VY, #Lam KP, #Tergaonkar V. The transcription complex p52-ETS1 is essential for germinal center formation. Nat Immunol. 2025 Sep;26(9):1553-1566. doi: 10.1038/s41590-025-02236-1. Epub 2025 Jul 25. PMID: 40715659.
#Hiraoka T, Aikawa S, Mashiko D, Nakagawa T, Shirai H, Hirota Y, Kimura H, #Ikawa M. An ex vivo uterine system captures implantation, embryogenesis, and trophoblast invasion via maternal-embryonic signaling. Nat Commun. 2025 Jul 1;16(1):5755. doi: 10.1038/s41467-025-60610-x. PMID: 40595542. PMC12217791.
Tokuoka Y, Endo T, Morikura T, Hiradate Y, #Ikawa M, #Funahashi A. Deep-learning-based automated prediction of mouse seminiferous tubule stage by using bright-field microscopy. Sci Rep. 2025 Jul 1;15(1):21849. doi: 10.1038/s41598-025-06727-x. PMID: 40594622. PMC12215975.
Chang HY, #Lu Y, Yamamoto K, Sun J, Shimada K, Hiradate Y, Fujihara Y, #Ikawa M. Mouse genome engineering uncovers 18 genes dispensable for male reproduction. Andrology. 2026 Mar;14(3):833-846. doi: 10.1111/andr.70088. Epub 2025 Jun 26. PMID: 40572022. PMC12917576.
Oyama Y, Ito S, Yuasa T, Ueda M, Chiba S, Nakagawa T, Izumi A, #Ikawa M, Koizumi N, #Okumura N. Generation of a Mouse Model of Fuchs Endothelial Corneal Dystrophy by Knock-in of CTG Trinucleotide Repeat Expansion in the TCF4 Gene. Invest Ophthalmol Vis Sci. 2025 Jun 2;66(6):18. doi: 10.1167/iovs.66.6.18. PMID: 40465262. PMC12151262.
Kitakaze H, #Miyata H, Oyama Y, Pan C, Kujime Y, Tsujimura G, Imanaka T, Kuribayashi S, Ueda N, Takezawa K, Fukuhara S, Nonomura N, #Ikawa M. KIF2C Deletion Causes Meiotic Abnormalities and Nonobstructive Azoospermia in Mice. Reprod Med Biol. 2025 May 27;24(1):e12659. doi: 10.1002/rmb2.12659. eCollection 2025 Jan-Dec. PMID: 40433108. PMC12107603.
Katsuma K, #Shimada K, Tonai S, Mashiko D, Iida-Norita R, Kaneda Y, Miyata H, #Ikawa M. The absence of both RIBC1 and RIBC2 induces decreased sperm motility and litter size in male mice. Andrology. 2026 Feb;14(2):545-554. doi: 10.1111/andr.70045. Epub 2025 Apr 23. PMID: 40265983. PMC12842877.
*Ito S, *Oyama Y, Yuasa T, Amano K, Onishi K, Izumi A, Jun AS, Ikawa M, Koizumi N, #Okumura N. Heterozygous Tcf4 Deficiency Mitigates Fuchs Endothelial Corneal Dystrophy Progression in a Mouse Model. Invest Ophthalmol Vis Sci. 2025 Apr 1;66(4):19. doi: 10.1167/iovs.66.4.19. PMID: 40197778. PMC11984510.
Fujimoto S, Endo M, Tonomura S, Tsuji F, Haraguchi H, Hasegawa K, Numao T, Izumi A, Tourtas T, Schlotzer-Schrehardt U, Kruse F, Oyama Y, Ikawa M, Jun AS, Koizumi N, #Okumura N. Therapeutic Potential of Emricasan, a Pan-Caspase Inhibitor, in Reducing Cell Death and Extracellular Matrix Accumulation in Fuchs Endothelial Corneal Dystrophy. Cells. 2025 Mar 27;14(7). pii: cells14070498. doi: 10.3390/cells14070498. PMID: 40214452. PMC11988121.
Lu Y, Ikawa M, #Tang S. Allosteric inhibition of the IZUMO1-JUNO fertilization complex by the naturally occurring antisperm antibody OBF13. Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2425952122. doi: 10.1073/pnas.2425952122. Epub 2025 Mar 5. PMID: 40042902. PMC11912406.
*Kaneda Y, *#Lu Y, *Sun J, Shimada K, Emori C, Noda T, Koyano T, Matsuyama M, #Miyata H, #Ikawa M. TEX38 localizes ZDHHC19 to the plasma membrane and regulates sperm head morphogenesis in mice. Proc Natl Acad Sci U S A. 2025 Mar 11;122(10):e2417943122. doi: 10.1073/pnas.2417943122. Epub 2025 Mar 3. PMID: 40030029. PMC11912386.
Suzuki Okutani M, Okamura S, Gis T, Sasaki H, Lee S, Kashiwabara A, Goto S, Matsumoto M, Yamawaki M, Miyazaki T, Nakagawa T, Ikawa M, Kamitani W, Takekawa S, Yamanishi K, #Ebina H. Immunogenicity and safety of a live-attenuated SARS-CoV-2 vaccine candidate based on multiple attenuation mechanisms. Elife. 2025 Feb 11;13. pii: 97532. doi: 10.7554/eLife.97532. PMID: 39932490. PMC11813227.
Nakagawa T, Emori C, #Ikawa M. Trophectoderm-specific gene manipulation using adeno-associated viral vectors. Exp Anim. 2025 Jul 11;74(3):310-318. doi: 10.1538/expanim.24-0165. Epub 2025 Jan 11. PMID: 39805595. PMC12270592.
Kamoshita M, Shirai H, Nakamura H, Kishimoto T, Hatanaka Y, Mashiko D, Esashika K, Yang J, Yamasaki S, Ogawa T, #Kimura H, #Ikawa M. Development of the membrane ceiling method for in vitro spermatogenesis. Sci Rep. 2025 Jan 3;15(1):625. doi: 10.1038/s41598-024-84965-1. PMID: 39753886. PMC11699200.
Kuribayashi S, #Fukuhara S, Kitakaze H, Tsujimura G, Imanaka T, Ueda N, Takezawa K, Ikawa M, Nonomura N. Intratesticular creatine maintains spermatogenesis by defining tight junctions. Sci Rep. 2024 Dec 28;14(1):30692. doi: 10.1038/s41598-024-77986-3. PMID: 39730393. PMC11680987.
Ishikawa-Yamauchi Y, Emori C, Mori H, Endo T, Kobayashi K, Watanabe Y, Sagara H, Nagata T, Motooka D, Ninomiya A, Ozawa M, #Ikawa M. Age-associated aberrations of the cumulus-oocyte interaction and in the zona pellucida structure reduce fertility in female mice. Commun Biol. 2024 Dec 24;7(1):1692. doi: 10.1038/s42003-024-07305-z. PMID: 39719529. PMC11668875.
*Aikawa S, *Hiraoka T, Matsuo M, Fukui Y, Fujita H, Saito-Fujita T, Shimizu-Hirota R, Takeda N, Hiratsuka D, He X, Ishizawa C, Iida R, Akaeda S, Harada M, Wada-Hiraike O, Ikawa M, Osuga Y, #Hirota Y. Spatiotemporal functions of leukemia inhibitory factor in embryo attachment and implantation chamber formation. Cell Death Discov. 2024 Nov 25;10(1):481. doi: 10.1038/s41420-024-02228-4. PMID: 39587062. PMC11589870.
Oyama Y, Shimada K, #Miyata H, Iida-Norita R, Emori C, Kamoshita M, Oura S, Katayama R, Matzuk MM, #Ikawa M. Inhibition of ROS1 activity with lorlatinib reversibly suppresses fertility in male mice. Andrology. 2024 Nov 20. doi: 10.1111/andr.13808. Online ahead of print. PMID: 39568175. PMC12476209.
Okada K, #Ito D, Morimoto S, Kato C, Oguma Y, Warita H, Suzuki N, Aoki M, Kuramoto J, Kobayashi R, Shinozaki M, Ikawa M, Nakahara J, Takahashi S, Nishimoto Y, Shibata S, #Okano H. Multiple lines of evidence for disruption of nuclear lamina and nucleoporins in FUS amyotrophic lateral sclerosis. Brain. 2024 Nov 4;147(11):3933-3948. doi: 10.1093/brain/awae224 PMID: 39312484. PMC11684083.
#*Deneke VE, *Blaha A, Lu Y, Suwita JP, Draper JM, Phan CS, Panser K, Schleiffer A, Jacob L, Humer T, Stejskal K, Krssakova G, Roitinger E, Handler D, Kamoshita M, Vance TDR, Wang X, Surm JM, Moran Y, Lee JE, Ikawa M, #Pauli A. A conserved fertilization complex bridges sperm and egg in vertebrates. Cell. 2024 Dec 12;187(25):7066-7078.e22. doi: 10.1016/j.cell.2024.09.035. Epub 2024 Oct 17. PMID: 39423812.
*Kim J, *Yu YS, Choi Y, Lee DH, Han S, Kwon J, Noda T, Ikawa M, Kim D, Kim H, Ballabio A, #Kim KI, #Baek SH. USF2 and TFEB compete in regulating lysosomal and autophagy genes. Nat Commun. 2024 Sep 27;15(1):8334. doi: 10.1038/s41467-024-52600-2. PMID: 39333072. PMC11436898.
Qiu Y, #Shimada K, Yamamoto K, #Ikawa M. Loss of CCDC188 causes male infertility with defects in the sperm head-neck connection in mice. Biol Reprod. 2024 Sep 18:ioae137. doi: 10.1093/biolre/ioae137. Online ahead of print. PMID: 39292630. PMC11736427.
Kawaguchi T, Hashimoto M, Nakagawa R, Minami R, Ikawa M, #Nakayama JI, #Ueda J. Comprehensive posttranslational modifications in the testis-specific histone variant H3t protein validated in tagged knock-in mice. Sci Rep. 2024 Sep 12;14(1):21305. doi: 10.1038/s41598-024-72362-7. PMID: 39266663. PMC11393354.
Lee J, Sasaki F, Koike E, Cho M, Lee Y, Dho SH, Lee J, Lee E, Toyohara E, Sunakawa M, Ishibashi M, Hung HH, Nishioka S, Komine R, Okura C, Shimizu M, Ikawa M, Yoshimura A, #Morita R, #Kim LK. Gelsolin alleviates rheumatoid arthritis by negatively regulating NLRP3 inflammasome activation. Cell Death Differ. 2024 Dec;31(12):1679-1694. doi: 10.1038/s41418-024-01367-6. Epub 2024 Aug 24. PMID: 39179640. PMC11618363.
#Kawai T, Morioka S, Miyata H, Andriani RT, Akter S, Toma G, Nakagawa T, Oyama Y, Iida-Norita R, Sasaki J, Watanabe M, Sakimura K, Ikawa M, Sasaki T, Okamura Y. The significance of electrical signals in maturing spermatozoa for phosphoinositide regulation through voltage-sensing phosphatase. Nat Commun. 2024 Aug 24;15(1):7289. doi: 10.1038/s41467-024-51755-2. PMID: 39181879. PMC11344830.
Kaneda Y, #Miyata H, Xu Z, Shimada K, Kamoshita M, Nakagawa T, Emori C, #Ikawa M. FBXO24 deletion causes abnormal accumulation of membraneless electron-dense granules in sperm flagella and male infertility. Elife. 2024 Aug 20:13:RP92794. doi: 10.7554/eLife.92794. PMID: 39163107. PMC11335345.
Yamamoto K, #Hiradate Y, #Ikawa M. Eighteen genes primarily expressed in the testis are not required for male fertility in mice. Biol Reprod. 2024 Aug 6:ioae119. doi: 10.1093/biolre/ioae119. Online ahead of print. PMID: 39105275. PMC11565233.
*Wang H, *Kobayashi H, Shimada K, Oura S, Oyama Y, Kitakaze H, Noda T, Yabuta N, #Miyata H, #Ikawa M. MYCBPAP is a central apparatus protein required for centrosome-nuclear envelope docking and sperm tail biogenesis in mice. J Cell Sci. 2024 Aug 2:jcs.261962. doi: 10.1242/jcs.261962. Online ahead of print. PMID: 39092789. PMC11385322.
Xu Z, Fujimoto Y, Sakamoto M, Ito D, Ikawa M, #Ishiuchi T. Kdm4d mutant mice show impaired sperm motility and subfertility. J Reprod Dev. 2024 Oct 1;70(5):320-326. doi: 10.1262/jrd.2024-039. Epub 2024 Jul 22. PMID: 39034148. PMC11461516.
*Nakanishi Y, *Izumi M, Matsushita H, Koyama Y, Diez D, Takamatsu H, Koyama S, Nishide M, Naito M, Mizuno Y, Yamaguchi Y, Mae T, Noda Y, Nakaya K, Nojima S, Sugihara F, Okuzaki D, Ikawa M, Shimada S, #Kang S, #Kumanogoh A. Semaphorin 6D tunes amygdalar circuits for emotional, metabolic, and inflammatory outputs. Neuron. 2024 Sep 4;112(17):2955-2972.e9. doi: 10.1016/j.neuron.2024.06.017. Epub 2024 Jul 12. PMID: 39002542.
Wang H, Iida-Norita R, Mashiko D, Pham AH, #Miyata H, #Ikawa M. Golgi associated RAB2 interactor protein family contributes to murine male fertility to various extents by assuring correct morphogenesis of sperm heads. PLoS Genet. 2024 Jun 27;20(6):e1011337. doi: 10.1371/journal.pgen.1011337. Online ahead of print. PMID: 38935810. PMC11236154.
Nguyen TTT, #Tokuhiro K, Shimada K, Wang H, Mashiko D, Tonai S, Kiyozumi D, #Ikawa M. Gene-deficient mouse model established by CRISPR/Cas9 system reveals 15 reproductive organ-enriched genes dispensable for male fertility. Front Cell Dev Biol. 2024 May 21:12:1411162. doi: 10.3389/fcell.2024.1411162. eCollection 2024. PMID: 38835510. PMC11148293.
Pham AH, Emori C, Ishikawa-Yamauchi Y, Tokuhiro K, Kamoshita M, Fujihara Y, #Ikawa M. Thirteen Ovary-Enriched Genes Are Individually Not Essential for Female Fertility in Mice. Cells. 2024 May 8;13(10):802. doi: 10.3390/cells13100802. PMID: 38786026. PMC11119756.
#Miyazaki H, Nishioka S, Yamanaka T, Abe M, Imamura Y, Miyasaka T, Kakuda N, Oohashi T, Shimogori T, Yamakawa K, Ikawa M, #Nukina N. Generation and characterization of cerebellar granule neurons specific knockout mice of Golli-MBP. Transgenic Res. 2024 Jun;33(3):99-117. doi: 10.1007/s11248-024-00382-0. Epub 2024 Apr 29. PMID: 38684589. PMC11176102.
Suzuki A, #Yabuta N, Shimada K, Mashiko D, Tokuhiro K, Oyama Y, Miyata H, Garcia TX, Matzuk MM, #Ikawa M. Individual disruption of 12 testis-enriched genes via the CRISPR/Cas9 system does not affect the fertility of male mice. J Reprod Immunol. 2024 Apr 29:163:104252. doi: 10.1016/j.jri.2024.104252. Online ahead of print. PMID: 38697008. PMC11390273.
#Metwally H, Elbrashy MM, Ozawa T, Okuyama K, White JT, Tulyeu J, Søndergaard JN, Wing JB, Muratsu A, Matsumoto H, Ikawa M, Kishi H, Taniuchi I, #Kishimoto T. Threonine phosphorylation of STAT1 restricts interferon signaling and promotes innate inflammatory responses. Proc Natl Acad Sci U S A. 2024 Apr 23;121(17):e2402226121. doi: 10.1073/pnas.2402226121. Epub 2024 Apr 15. PMID: 38621137. PMC11046697.
*Akamine T, *Terabayashi T, Sasaki T, Hayashi R, Abe I, Hirayama F, Nureki SI, Ikawa M, Miyata H, Tokunaga A, Kobayashi T, Hanada K, Thumkeo D, Narumiya S, #Ishizaki T. Conditional deficiency of Rho-associated kinases disrupts endothelial cell junctions and impairs respiratory function in adult mice. FEBS Open Bio. 2024 Jun;14(6):906-921. doi: 10.1002/2211-5463.13802. Epub 2024 Apr 11. PMID: 38604990. PMC11148122.
#Noda T, Shinohara H, Kobayashi S, Taira A, Oura S, Tahara D, Tokuyasu M, Araki K, #Ikawa M. Multiple genes in the Pate5-13 genomic region contribute to ADAM3 processing. Biol Reprod. 2024 Apr 11;110(4):750-760. doi: 10.1093/biolre/ioae008. PMID: 38217862. PMC11017121.
Liao Z, Tang S, Nozawa K, Shimada K, Ikawa M, #Monsivais D, #Matzuk M. Affinity-tagged SMAD1 and SMAD5 mouse lines reveal transcriptional reprogramming mechanisms during early pregnancy. Elife. 2024 Mar 27:12:RP91434. doi: 10.7554/eLife.91434. PMID: 38536963. PMC10972565.
Nishio S, Emori C, Wiseman B, Fahrenkamp D, Dioguardi E, Zamora-Caballero S, Bokhove M, Han L, Stsiapanava A, Algarra B, Lu Y, Kodani M, Bainbridge R E, Komondor K M, Carlson A E, Landreh M, Sanctis D, Yasumasu S, Ikawa M, #Jovine L. ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat. Cell. 2024 Mar 14;187(6):1440-1459.e24. doi: 10.1016/j.cell.2024.02.013. PMID: 38490181. PMC10976854.
#Fujihara Y, Miyata H, Abbasi F, Larasati T, Nozawa K, Yu Z, Ikawa M, Matzuk MM. Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida. PNAS Nexus. 2024 Mar 12;3(3):pgae108. doi: 10.1093/pnasnexus/pgae108. eCollection 2024 Mar. PMID: 38516277. PMC10957234.
#Endo T, Kobayashi K, Matsumura T, Emori C, Ozawa M, Kawamoto S, Okuzaki D, Shimada K, Miyata H, Shimada K, Kodani M, Ishikawa-Yamauchi Y, Motooka D, Hara E, #Ikawa M. Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice. Commun Biol. 2024 Jan 4;7(1):16. doi: 10.1038/s42003-023-05685-2. PMID: 38177279. PMC10766604.
Sammer B, Schmid P, Miyata H, Kazi S, Honkimaa AL, Petrov P, Kapiainen E, Miinalainen I, Izzi V, Ikawa M, #Prunskaite-Hyyrylainen R. Ccer1 is a spermatid-specific gene required for spermatogenesis and male fertility. Int J Dev Biol. 2024;68(4):251-262. doi: 10.1387/ijdb.240205rp. PMID: 39868420.
Ogawa Y, #Lu Y, Kiyozumi D, Chang HY, #Ikawa M. CRISPR/Cas9-mediated genome editing reveals seven testis-enriched transmembrane glycoproteins dispensable for male fertility in mice. Andrology. 2025 Jul;13(5):1251-1260. doi: 10.1111/andr.13564. Epub 2023 Dec 12. PMID: 38084666. PMC11166886.
*Emori C, *Kodani M, Abbasi F, Mori M, #Ikawa M. PABPN1L is required for maternal mRNA degradation after meiosis resumption. J Reprod Dev. 2024 Feb 19;70(1):10-17. doi: 10.1262/jrd.2023-077. Epub 2023 Dec 7. PMID: 38057116. PMC10902638.
#Ozawa M, Mori H, Endo T, Ishikawa-Yamauchi Y, Motooka D, Emori C, #Ikawa M. Age-related decline in spermatogenic activity accompanied with endothelial cell senescence in male mice. iScience. 2023 Nov 13;26(12):108456. doi: 10.1016/j.isci.2023.108456. eCollection 2023 Dec 15. PMID: 38077127. PMC10700819.
#Shimada K, #Ikawa M. CCDC183 is essential for cytoplasmic invagination around the flagellum during spermiogenesis and male fertility. Development. 2023 Nov 1;150(21). pii: 334158. doi: 10.1242/dev.201724. Epub 2023 Oct 30. PMID: 37882665. PMC10629680.
#Kiyozumi D, Ikawa M. Adhesion G protein-coupled receptor G2 is dispensable for lumicrine signaling regulating epididymal initial segment differentiation and gene expressiondagger. Biol Reprod. 2023 Oct 13;109(4):474-481. doi: 10.1093/biolre/ioad087. PMID: 37531264. PMC10577274.
Huang X, Miyata H, Wang H, Mori G, Iida-Norita R, Ikawa M, Percudani R, #Chung JJ. A CUG-initiated CATSPERtheta functions in the CatSper channel assembly and serves as a checkpoint for flagellar trafficking. Proc Natl Acad Sci U S A. 2023 Sep 26;120(39):e2304409120. doi: 10.1073/pnas.2304409120. Epub 2023 Sep 19. PMID: 37725640. PMC10523455.
#Shinzawa K, Matsumoto S, Sada R, Harada A, Saitoh K, Kato K, Ikeda S, Hirayama A, Yokoi K, Tanemura A, Nimura K, Ikawa M, Soga T, #Kikuchi A. GREB1 isoform 4 is specifically transcribed by MITF and required for melanoma proliferation. Oncogene. 2023 Oct;42(42):3142-3156. doi: 10.1038/s41388-023-02803-6. Epub 2023 Sep 1. PMID: 37658191. PMC10575781.
Ihashi S, Hamanaka M, Kaji M, Mori R, Nishizaki S, Mori M, Imasato Y, Inoue K, Matoba S, Ogonuki N, Takasu A, Nakamura M, Matsumoto K, Anzai M, Ogura A, Ikawa M, #Miyamoto K. Incomplete activation of Alyref and Gabpb1 leads to preimplantation arrest in cloned mouse embryos. Life Sci Alliance. 2023 Aug 28;6(11). pii: 6/11/e202302296. doi: 10.26508/lsa.202302296. Print 2023 Nov. PMID: 37640449. PMC10462978.
Kent K, Nozawa K, Sutton C, Daniel F, Ikawa M, Garcia TX, #Matzuk MM. CUB domains are not required for OVCH2 function in sperm maturation in the mouse epididymis. Andrology. 2024 Mar;12(3):682-697. doi: 10.1111/andr.13508. Epub 2023 Aug 8. PMID: 37551853. PMC10850435.
Nozawa K, Liao Z, Satouh Y, Geng T, Ikawa M, #Monsivais D, #Matzuk MM. Oocyte-specific Wee1-like protein kinase 2 is dispensable for fertility in mice. PLoS One. 2023 Aug 1;18(8):e0289083. doi: 10.1371/journal.pone.0289083. eCollection 2023. PMID: 37527245. PMC10393137.
Sutton C, Nozawa K, Kent K, Saltzman A, Leng M, Nagarajan S, Malovannaya A, Ikawa M, Garcia TX, #Matzuk MM. Molecular dissection and testing of PRSS37 function through LC-MS/MS and the generation of a PRSS37 humanized mouse model. Sci Rep. 2023 Jul 14;13(1):11374. doi: 10.1038/s41598-023-37700-1. PMID: 37452050. PMC10349139.
#Shimada K, Lu Y, #Ikawa M. Disruption of testis-enriched cytochrome c oxidase subunit COX6B2 but not COX8C leads to subfertility. Exp Anim. 2024 Feb 14;73(1):1-10. doi: 10.1538/expanim.23-0055. Epub 2023 Jul 10. PMID: 37423748. PMC10877148.
#Kiyozumi D, Shimada K, Chalick M, Emori C, Kodani M, Oura S, Noda T, Endo T, Matzuk MM, #Wreschner DH, #Ikawa M. A small secreted protein NICOL regulates lumicrine-mediated sperm maturation and male fertility. Nat Commun. 2023 Apr 24;14(1):2354. doi: 10.1038/s41467-023-37984-x. PMID: 37095084. PMC10125973.
#Funaya S, Wang Y, Suzuki MG, Ikawa M, #Aoki F. Involvement of linker histone variant H1a in the regulation of early preimplantation development in mice. J Reprod Dev. 2023 Jun 6;69(3):178-182. doi: 10.1262/jrd.2023-013. Epub 2023 Apr 17. PMID: 37062716. PMC10267588.
Shirai T, Nakai A, Ando E, Fujimoto J, Leach S, Arimori T, Higo D, van Eerden FJ, Tulyeu J, Liu YC, Okuzaki D, Murayama MA, Miyata H, Nunomura K, Lin B, Tani A, Kumanogoh A, Ikawa M, Wing JB, Standley DM, Takagi J, #Suzuki K. Celastrol suppresses humoral immune responses and autoimmunity by targeting the COMMD3/8 complex. Sci Immunol. 2023 Mar 31;8(81):eadc9324. doi: 10.1126/sciimmunol.adc9324. Epub 2023 Mar 31. PMID: 37000855.
#Shimada K, Park S, Oura S, Noda T, Morohoshi A, #Matzuk MM, #Ikawa M. TSKS localizes to nuage in spermatids and regulates cytoplasmic elimination during spermiation. Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2221762120. doi: 10.1073/pnas.2221762120. Epub 2023 Mar 7. PMID: 36881620. PMC10242716.
Miyake T, Inoue Y, Shao X, Seta T, Aoki Y, Nguyen Pham KT, Shichino Y, Sasaki J, Sasaki T, Ikawa M, Yamaguchi Y, Okamura H, Iwasaki S, #Doi M. Minimal upstream open reading frame of Per2 mediates phase fitness of the circadian clock to day/night physiological body temperature rhythm. Cell Rep. 2023 Mar 28;42(3):112157. doi: 10.1016/j.celrep.2023.112157. Epub 2023 Mar 6. PMID: 36882059.
Ferrer P, Upadhyay S, Ikawa M, #Clement TM. Testis-specific actin-like 7A (ACTL7A) is an indispensable protein for subacrosomal-associated F-actin formation, acrosomal anchoring, and male fertility. Mol Hum Reprod. 2023 Feb 28;29(3). pii: 7025500. doi: 10.1093/molehr/gaad005. PMID: 36734600. PMC9976968.
Kuribayashi S, #Fukuhara S, Tsujimura G, Imanaka T, Okada K, Ueda N, Takezawa K, Kiuchi H, Saito S, Takahashi Y, Kioka H, Oura S, Shimada K, Ikawa M, Nonomura N. Evaluation of the efficacy of creatine chemical exchange saturation transfer imaging in assessing testicular maturity. Reprod Med Biol. 2023 Feb 23;22(1):e12507. doi: 10.1002/rmb2.12507. eCollection 2023 Jan-Dec. PMID: 36845001. PMC9949363.
Lu Y, Nagamori I, Kobayashi H, Kojima-Kita K, Shirane K, Chang HY, Nishimura T, Koyano T, Yu Z, Castaneda JM, Matsuyama M, #Kuramochi-Miyagawa S, #Matzuk MM, #Ikawa M. ADAD2 functions in spermiogenesis and piRNA biogenesis in mice. Andrology. 2023 May;11(4):698-709. doi: 10.1111/andr.13400. Epub 2023 Feb 14. PMID: 36698249. PMC10073342.
Lu Y, Shimada K, Tang S, Zhang J, Ogawa Y, Noda T, Shibuya H, #Ikawa M. 1700029I15Rik orchestrates the biosynthesis of acrosomal membrane proteins required for sperm-egg interaction. Proc Natl Acad Sci U S A. 2023 Feb 21;120(8):e2207263120. doi: 10.1073/pnas.2207263120. Epub 2023 Feb 14. PMID: 36787362. PMC9974436.
*Morohoshi A, *#Miyata H, Tokuhiro K, Iida-Norita R, Noda T, Fujihara Y, #Ikawa M. Testis-enriched ferlin, FER1L5, is required for Ca(2+)-activated acrosome reaction and male fertility. Sci Adv. 2023 Jan 25;9(4):eade7607. doi: 10.1126/sciadv.ade7607. Epub 2023 Jan 25. PMID: 36696506. PMC9876558.
Kaneda Y, #Miyata H, Shimada K, Oura S, #Ikawa M. Testis-specific proteins, TSNAXIP1 and 1700010I14RIK, are important for sperm motility and male fertility in mice. Andrology. 2023 Jul;11(5):799-807. doi: 10.1111/andr.13378. Epub 2023 Jan 16. PMID: 36598146. PMC10972718.
Morita M, Yoneda A, Tokunoh N, Masaki T, Shirakura K, Kinoshita M, Hashimoto R, Shigesada N, Takahashi J, Tachibana M, Tanaka S, Obana M, Hino N, Ikawa M, Tsujikawa K, Ono C, Matsuura Y, Kidoya H, Takakura N, Kubota Y, Doi T, Takayama K, Yoshioka Y, Fujio Y, #Okada Y. Upregulation of Robo4 expression by SMAD signaling suppresses vascular permeability and mortality in endotoxemia and COVID-19 models. Proc Natl Acad Sci U S A. 2023 Jan 17;120(3):e2213317120. doi: 10.1073/pnas.2213317120. Epub 2023 Jan 12. PMID: 36634143. PMC9934020.
Oura S, Ninomiya A, Sugihara F, Matzuk MM, #Ikawa M. Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Sci Rep. 2022 Dec 23;12(1):22198. doi: 10.1038/s41598-022-26501-7. PMID: 36564444. PMC9789103.
Iida-Norita R, Miyata H, Kaneda Y, Emori C, Noda T, Nakagawa T, #Matzuk MM, #Ikawa M. Generation of humanized LDHC knock-in mice as a tool to assess human LDHC-targeting contraceptive drugs. Andrology. 2023 Jul;11(5):840-848. doi: 10.1111/andr.13359. Epub 2022 Dec 19. PMID: 36464740.
#Ozawa M, Taguchi J, Katsuma K, Ishikawa-Yamauchi Y, Kikuchi M, Sakamoto R, Yamada Y, #Ikawa M. Efficient simultaneous double DNA knock-in in murine embryonic stem cells by CRISPR/Cas9 ribonucleoprotein-mediated circular plasmid targeting for generating gene-manipulated mice. Sci Rep. 2022 Dec 13;12(1):21558. doi: 10.1038/s41598-022-26107-z. PMID: 36513736. PMC9748034.
Sakurai N, #Fujihara Y, Kobayashi K, Ikawa M. CRISPR/Cas9-mediated disruption of lipocalins, Ly6g5b, and Ly6g5c causes male subfertility in mice. Andrology. 2024 Jul;12(5):981-990. doi: 10.1111/andr.13350. Epub 2022 Dec 10. PMID: 36428102. PMC10506895.
Nozawa K, Garcia TX, Kent K, Leng M, Jain A, Malovannaya A, Yuan F, Yu Z, Ikawa M, #Matzuk MM. Testis-specific serine kinase 3 is required for sperm morphogenesis and male fertility. Andrology. 2023 Jul;11(5):826-839. doi: 10.1111/andr.13314. Epub 2022 Nov 16. PMID: 36306217. PMC10267670.
Yokoyama M, Fujita T, Kadonosawa Y, Tatara Y, Motooka D, Ikawa M, Fujii H, #Yokoayama Y. Development of transgenic mice overexpressing mouse carbonyl reductase 1. Mol Biol Rep. 2023 Jan;50(1):531-540. doi: 10.1007/s11033-022-07994-x. Epub 2022 Nov 9. PMID: 36352178.
#Fujihara Y, Kobayashi K, Abbasi F, Endo T, Yu Z, Ikawa M, Matzuk MM. PDCL2 is essential for sperm acrosome formation and male fertility in mice. Andrology. 2023 Jul;11(5):789-798. doi: 10.1111/andr.13329. Epub 2022 Nov 8. PMID: 36278277. PMC10123174.
Oura S, Mori H, #Ikawa M. Genome editing in mice and its application to the study of spermatogenesis. Gene and Genome Editing. Available online 30 October 2022, 100014.
Irie M, Itoh J, Matsuzawa A, Ikawa M, Kiyonari H, Kihara M, Suzuki T, Hiraoka Y, #Ishino F, #Kaneko-Ishino T. Retrovirus-derived RTL5 and RTL6 genes are novel constituents of the innate immune system in the eutherian brain. Development. 2022 Sep 15;149(18). pii: 276855. doi: 10.1242/dev.200976. Epub 2022 Sep 27. PMID: 36162816. PMC9641642.
*Tang S, *Lu Y, Skinner WM, Sanyal M, Lishko PV, #Ikawa M, #Kim PS. Human sperm TMEM95 binds eggs and facilitates membrane fusion. Proc Natl Acad Sci U S A. 2022 Oct 4;119(40):e2207805119. doi: 10.1073/pnas.2207805119. Epub 2022 Sep 26. PMID: 36161911. PMC9546558.
Hashimoto R, Takahashi J, Shirakura K, Funatsu R, Kosugi K, Deguchi S, Yamamoto M, Tsunoda Y, Morita M, Muraoka K, Tanaka M, Kanbara T, Tanaka S, Tamiya S, Tokunoh N, Kawai A, Ikawa M, Ono C, Tachibana K, Kondoh M, Obana M, Matsuura Y, Ohsumi A, Noda T, Yamamoto T, Yoshioka Y, Torisawa YS, Date H, Fujio Y, Nagao M, #Takayama K, #Okada Y. SARS-CoV-2 disrupts respiratory vascular barriers by suppressing Claudin-5 expression. Sci Adv. 2022 Sep 23;8(38):eabo6783. doi: 10.1126/sciadv.abo6783. Epub 2022 Sep 21. PMID: 36129989. PMC9491726.
Vondrakova J, Frolikova M, Ded L, Cerny J, Postlerova P, Palenikova V, Simonik O, Nahacka Z, Basus K, Valaskova E, Machan R, Pacey A, Holubcova Z, Koubek P, Ezrova Z, Park S, Liu R, Partha R, Clark N, Neuzil J, Ikawa M, Erickson K, Lam KS, #Moore H, #Komrskova K. MAIA, Fc receptor-like 3, supersedes JUNO as IZUMO1 receptor during human fertilization. Sci Adv. 2022 Sep 9;8(36):eabn0047. doi: 10.1126/sciadv.abn0047. Epub 2022 Sep 7. PMID: 36070373. PMC9451160.
#Kobayashi D, Watarai T, Ozawa M, Kanda Y, Saika F, Kiguchi N, Takeuchi A, Ikawa M, Matsuzaki S, #Katakai T. Tas2R signaling enhances mouse neutrophil migration via a ROCK-dependent pathway. Front Immunol. 2022 Aug 18;13:973880. doi: 10.3389/fimmu.2022.973880. eCollection 2022. PMID: 36059440. PMC9436316.
Nozawa K, Fujihara Y, Devlin DJ, Deras RE, Kent K, Larina IV, Umezu K, Yu Z, Sutton CM, Ye Q, Dean LK, Emori C, Ikawa M, Garcia TX, #Matzuk MM. The testis-specific E3 ubiquitin ligase RNF133 is required for fecundity in mice. BMC Biol. 2022 Jul 13;20(1):161. doi: 10.1186/s12915-022-01368-2. PMID: 35831855. PMC9277888.
#Fujiki F, Morimoto S, Katsuhara A, Okuda A, Ogawa S, Ueda E, Miyazaki M, Isotani A, Ikawa M, Nishida S, Nakajima H, Tsuboi A, Oka Y, Nakata J, Hosen N, Kumanogoh A, Oji Y, #Sugiyama H. T Cell-Intrinsic Vitamin A Metabolism and Its Signaling Are Targets for Memory T Cell-Based Cancer Immunotherapy. Front Immunol. 2022 Jun 30;13:935465. doi: 10.3389/fimmu.2022.935465. eCollection 2022. PMID: 35844620. PMC9280205.
Yamauchi Y, Matsumura T, Bakse J, Holmlund H, Blanchet G, Carrot E, Ikawa M, #Ward MA. Loss of mouse Y chromosome gene Zfy1 and Zfy2 leads to spermatogenesis impairment, sperm defects, and infertility. Biol Reprod. 2022 Jun 13;106(6):1312-1326. doi: 10.1093/biolre/ioac057. PMID: 35293998. PMC9199016.
Kuwayama R, Suzuki K, Nakamura J, Aizawa E, Yoshioka Y, Ikawa M, Nabatame S, Inoue KI, Shimmyo Y, Ozono K, Kinoshita T, #Murakami Y. Establishment of mouse model of inherited PIGO deficiency and therapeutic potential of AAV-based gene therapy. Nat Commun. 2022 Jun 3;13(1):3107. doi: 10.1038/s41467-022-30847-x. PMID: 35661110. PMC9166810.
Oura S, Hino T, Satoh T, Noda T, Koyano T, Isotani A, Matsuyama M, Akira S, Ishiguro KI, #Ikawa M. Trim41 is required to regulate chromosome axis protein dynamics and meiosis in male mice. PLoS Genet. 2022 Jun 1;18(6):e1010241. doi: 10.1371/journal.pgen.1010241. eCollection 2022 Jun. PMID: 35648791. PMC9191731.
Kaneda Y, #Miyata H, Shimada K, Oyama Y, Iida-Norita R, #Ikawa M. IRGC1, a testis-enriched immunity related GTPase, is important for fibrous sheath integrity and sperm motility in mice. Dev Biol. 2022 Aug;488:104-113. doi: 10.1016/j.ydbio.2022.05.011. Epub 2022 May 23. PMID: 35618043. PMC9232189.
Oyama Y, #Miyata H, Shimada K, Larasati T, Fujihara Y, #Ikawa M. TULP2 deletion mice exhibit abnormal outer dense fiber structure and male infertility. Reprod Med Biol. 2022 May 23;21(1):e12467. doi: 10.1002/rmb2.12467. eCollection 2022 Jan-Dec. PMID: 35619658. PMC9126596.
Oyama Y, #Miyata H, Shimada K, Fujihara Y, Tokuhiro K, Garcia TX, Matzuk MM, #Ikawa M. CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched genes dispensable for male fertility in mice. Asian J Androl. 2022 May-Jun;24(3):266-272. doi: 10.4103/aja.aja_63_21. PMID: 34290169. PMC9226692.
Noda T, Blaha A, Fujihara Y, Gert KR, Emori C, Deneke VE, Oura S, Panser K, Lu Y, Berent S, Kodani M, Cabrera-Quio LE, #Pauli A, #Ikawa M. Sperm membrane proteins DCST1 and DCST2 are required for sperm-egg interaction in mice and fish. Commun Biol. 2022 Apr 7;5(1):332. doi: 10.1038/s42003-022-03289-w. PMID: 35393517. PMC8989947.
Hiramatsu Y, Suzuki K, Nishida T, Onoda N, Satoh T, Akira S, Ikawa M, Ikeda H, Kamei J, Derouiche S, Tominaga M, #Horiguchi Y. The Mechanism of Pertussis Cough Revealed by the Mouse-Coughing Model. mBio. 2022 Apr 26;13(2):e0319721. doi: 10.1128/mbio.03197-21. Epub 2022 Mar 31. PMID: 35357202. PMC9040802.
Ali El Hussien M, Tsai CY, #Satouh Y, Motooka D, Okuzaki D, Ikawa M, #Kikutani H, #Sakakibara S. Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice. Int Immunol. 2022 Mar 25;34(4):207-223. doi: 10.1093/intimm/dxab111. PMID: 34865040.
Mise S, #Matsumoto A, Shimada K, Hosaka T, Takahashi M, Ichihara K, Shimizu H, Shiraishi C, Saito D, Suyama M, Yasuda T, Ide T, Izumi Y, Bamba T, Kimura-Someya T, Shirouzu M, Miyata H, Ikawa M, #Nakayama KI. Kastor and Polluks polypeptides encoded by a single gene locus cooperatively regulate VDAC and spermatogenesis. Nat Commun. 2022 Feb 28;13(1):1071. doi: 10.1038/s41467-022-28677-y. PMID: 35228556. PMC8885739.
Hirata T, Kobayashi A, Furuse T, Yamada I, Tamura M, Tomita H, Tokoro Y, Ninomiya A, Fujihara Y, Ikawa M, Maeda Y, Murakami Y, #Kizuka Y, #Kinoshita T. Loss of the N-acetylgalactosamine side chain of the GPI-anchor impairs bone formation and brain functions and accelerates the prion disease pathology. J Biol Chem. 2022 Mar;298(3):101720. doi: 10.1016/j.jbc.2022.101720. Epub 2022 Feb 11. PMID: 35151686. PMC8913354.
Matsumura T, Noda T, Satouh Y, Morohoshi A, Yuri S, Ogawa M, Lu Y, Isotani A, #Ikawa M. Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma in Mice and Rats. Front Cell Dev Biol. 2022 Jan 12;9:810118. doi: 10.3389/fcell.2021.810118. eCollection 2021. PMID: 35096839. PMC8790511.
Hwang JY, Wang H, Lu Y, Ikawa M, #Chung JJ. C2cd6-encoded CatSpertau targets sperm calcium channel to Ca(2+) signaling domains in the flagellar membrane. Cell Rep. 2022 Jan 18;38(3):110226. doi: 10.1016/j.celrep.2021.110226. Epub 2022 Jan 7. PMID: 34998468. PMC8857959.
#Harada YU, Amo H, Ono T, Murai H, Sasaki T, Shioda T, Ikawa M, Hirata Y. Inactivation of SARS-CoV-2 by Commercially Available Disinfectants and Cleaners. Biocontrol Sci. 2022;27(4):223-228. doi: 10.4265/bio.27.223. PMID: 36567119.
#Castaneda JM, Shimada K, Satouh Y, Yu Z, Ikawa M, Matzuk MM. FAM209 associates with DPY19L2, and is required for sperm acrosome biogenesis and fertility in mice. J Cell Sci. 2021 Nov 1;134(21). pii: 272021. doi: 10.1242/jcs.259206. Epub 2021 Nov 1. PMID: 34471926. PMC8627553.
Fukui Y, #Hirota Y, Saito-Fujita T, Aikawa S, Hiraoka T, Kaku T, Hirata T, Akaeda S, Matsuo M, Shimizu-Hirota R, Takeda N, Ikawa M, Osuga Y. Uterine Epithelial LIF Receptors Contribute to Implantation Chamber Formation in Blastocyst Attachment. Endocrinology. 2021 Nov 1;162(11). pii: 6353290. doi: 10.1210/endocr/bqab169. PMID: 34402888.
*Morohoshi A, *Miyata H, Oyama Y, Oura S, Noda T, #Ikawa M. FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice. Development. 2021 Nov 1;148(21). pii: 272654. doi: 10.1242/dev.199644. Epub 2021 Oct 29. PMID: 34714330. PMC8602946.
*Zhang X, *Sun J, *Lu Y, *Zhang J, Shimada K, Noda T, Zhao S, Koyano T, Matsuyama M, Zhou S, Wu J, #Ikawa M, #Liu M. LRRC23 is a conserved component of the radial spoke that is necessary for sperm motility and male fertility in mice. J Cell Sci. 2021 Oct 15;134(20). pii: 272563. doi: 10.1242/jcs.259381. Epub 2021 Oct 21. PMID: 34585727. PMC10658914.
#Kojima-Kita K, Kuramochi-Miyagawa S, Nakayama M, Miyata H, Jacobsen SE, Ikawa M, Koseki H, #Nakano T. MORC3, a novel MIWI2 association partner, as an epigenetic regulator of piRNA dependent transposon silencing in male germ cells. Sci Rep. 2021 Oct 14;11(1):20472. doi: 10.1038/s41598-021-98940-7. PMID: 34650118. PMC8516955.
*Fujihara Y, *Herberg S, Blaha A, Panser K, Kobayashi K, Larasati T, Novatchkova M, Theussl HC, Olszanska O, #Ikawa M, #Pauli A. The conserved fertility factor SPACA4/Bouncer has divergent modes of action in vertebrate fertilization. Proc Natl Acad Sci U S A. 2021 Sep 28;118(39). pii: 2108777118. doi: 10.1073/pnas.2108777118. PMID: 34556579. PMC8488580.
#Miyata H, Oyama Y, Kaneda Y, #Ikawa M. The motor domain of testis-enriched kinesin KIF9 is essential for its localization in the mouse flagellum. Exp Anim. 2022 Feb 9;71(1):46-52. doi: 10.1538/expanim.21-0082. Epub 2021 Sep 15. PMID: 34526446. PMC8828407.
*Segawa K, *Kikuchi A, Noji T, Sugiura Y, Hiraga K, Suzuki C, Haginoya K, Kobayashi Y, Matsunaga M, Ochiai Y, Yamada K, Nishimura T, Iwasawa S, Shoji W, Sugihara F, Nishino K, Kosako H, Ikawa M, Uchiyama Y, Suematsu M, Ishikita H, Kure S, #Nagata S. A sublethal ATP11A mutation associated with neurological deterioration causes aberrant phosphatidylcholine flipping in plasma membranes. J Clin Invest. 2021 Sep 15;131(18). pii: e148005. doi: 10.1172/JCI148005. PMID: 34403372. PMC8439608.
Miyata H, Oura S, Morohoshi A, Shimada K, Mashiko D, Oyama Y, Kaneda Y, Matsumura T, Abbasi F, #Ikawa M. SPATA33 localizes calcineurin to the mitochondria and regulates sperm motility in mice. Proc Natl Acad Sci U S A. 2021 Aug 31;118(35). pii: 2106673118. doi: 10.1073/pnas.2106673118. PMID: 34446558. PMC8536318.
#Mori M, Yao T, Mishina T, Endoh H, Tanaka M, Yonezawa N, Shimamoto Y, Yonemura S, Yamagata K, Kitajima TS, #Ikawa M. RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization. J Cell Biol. 2021 Oct 4;220(10). pii: 212591. doi: 10.1083/jcb.202012001. Epub 2021 Aug 23. PMID: 34424312. PMC8404465.
Yamamuro T, #Nakamura S, Yamano Y, Endo T, Yanagawa K, Tokumura A, Matsumura T, Kobayashi K, Mori H, Enokidani Y, Yoshida G, Imoto H, Kawabata T, Hamasaki M, Kuma A, Kuribayashi S, Takezawa K, Okada Y, Ozawa M, Fukuhara S, Shinohara T, Ikawa M, #Yoshimori T. Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function. PLoS Genet. 2021 Aug 5;17(8):e1009688. doi: 10.1371/journal.pgen.1009688. eCollection 2021 Aug. PMID: 34351902. PMC8341604.
#Yoshida K, Fujita SI, Isotani A, Kudo T, Takahashi S, Ikawa M, Shiba D, Shirakawa M, Muratani M, Ishii S. Intergenerational effect of short-term spaceflight in mice. iScience. 2021 Jun 25;24(7):102773. doi: 10.1016/j.isci.2021.102773. eCollection 2021 Jul 23. PMID: 34278272. PMC8271179.
Oura S, Noda T, Morimura N, Hitoshi S, Nishimasu H, Nagai Y, Nureki O, #Ikawa M. Precise CAG repeat contraction in a Huntington's Disease mouse model is enabled by gene editing with SpCas9-NG. Commun Biol. 2021 Jun 23;4(1):771. doi: 10.1038/s42003-021-02304-w. PMID: 34163001. PMC8222283.
#Monsivais D, Nagashima T, Prunskaite-Hyyryläinen R, Nozawa K, Shimada K, Tang S, Hamor C, Agno JE, Chen F, Masand RP, Young SL, Creighton CJ, DeMayo FJ, Ikawa M, Lee SJ, #Matzuk MM. Endometrial receptivity and implantation require uterine BMP signaling through an ACVR2A-SMAD1/SMAD5 axis. Nat Commun. 2021 Jun 7;12(1):3386. doi: 10.1038/s41467-021-23571-5. PMID: 34099644. PMC8184938.
Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro KI, #Ikawa M. KCTD19 and its associated protein ZFP541 are independently essential for meiosis in male mice. PLoS Genet. 2021 May 7;17(5):e1009412. doi: 10.1371/journal.pgen.1009412. eCollection 2021 May. PMID: 33961623. PMC8104389.
Shibata T, Ikawa M, Sakasai R, Ishigaki Y, Kiyokawa E, Iwabuchi K, Singh DP, Sasaki H, #Kubo E. Lens-specific conditional knockout of tropomyosin 1 gene in mice causes abnormal fiber differentiation and lens opacity. Mech Ageing Dev. 2021 Jun;196:111492. doi: 10.1016/j.mad.2021.111492. Epub 2021 Apr 15. PMID: 33862037.
Kishimoto Y, Nishiura I, Hirata W, Yuri S, Yamamoto N, Ikawa M, #Isotani A. A novel tissue specific alternative splicing variant mitigates phenotypes in Ets2 frame-shift mutant models. Sci Rep. 2021 Apr 15;11(1):8297. doi: 10.1038/s41598-021-87751-5. PMID: 33859300. PMC8050053.
Matsuo T, Isosaka T, Hayashi Y, Tang L, Doi A, Yasuda A, Hayashi M, Lee CY, Cao L, Kutsuna N, Matsunaga S, Matsuda T, Yao I, Setou M, Kanagawa D, Higasa K, Ikawa M, #Liu Q, #Kobayakawa R, #Kobayakawa K. Thiazoline-related innate fear stimuli orchestrate hypothermia and anti- hypoxia via sensory TRPA1 activation. Nat Commun. 2021 Apr 6;12(1):2074. doi: 10.1038/s41467-021-22205-0. PMID: 33824316. PMC8024280.
*#Fukuda E, *Tanaka H, Yamaguchi K, Takasaka M, Kawamura Y, Okuda H, Isotani A, Ikawa M, Shapiro VS, Tsuchida J, Okada Y, Tsujimura A, Miyagawa Y, Fukuhara S, Kawakami Y, Wada M, Nishimune Y, Goshima N. Identification and characterization of the antigen recognized by the germ cell mAb TRA98 using a human comprehensive wet protein array. Genes Cells. 2021 Mar;26(3):180-189. doi: 10.1111/gtc.12832. PMID: 33527666.
Shimada K, Park S, Miyata H, Yu Z, Morohoshi A, Oura S, #Matzuk MM, #Ikawa M. ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility. Proc Natl Acad Sci U S A. 2021 Feb 9;118(6). pii: 2018355118. doi: 10.1073/pnas.2018355118. PMID: 33536340. PMC8017931.
Zhou Z, Okamoto K, Onodera J, Hiragi T, Andoh M, Ikawa M, Tanaka KF, Ikegaya Y, #Koyama R. Astrocytic cAMP modulates memory via synaptic plasticity. Proc Natl Acad Sci U S A. 2021 Jan 19;118(3). pii: 2016584118. doi: 10.1073/pnas.2016584118. PMID: 33452135. PMC7826339.
*Akıncılar SC, *Wu L, Ng QF, Chua JYH, Unal B, Noda T, Chor WHJ, Ikawa M, #Tergaonkar V. NAIL: an evolutionarily conserved lncRNA essential for licensing coordinated activation of p38 and NFkappaB in colitis. Gut. 2021 Oct;70(10):1857-1871. doi: 10.1136/gutjnl-2020-322980. Epub 2020 Nov 25. PMID: 33239342. PMC8458091.
#Miyamoto Y, Sasaki M, Miyata H, Monobe Y, Nagai M, Otani M, Whiley PAF, Morohoshi A, Oki S, Matsuda J, Akagi KI, Adachi J, Okabe M, Ikawa M, Yoneda Y, Loveland KL, #Oka M. Genetic loss of importin alpha4 causes abnormal sperm morphology and impacts on male fertility in mouse. FASEB J. 2020 Dec;34(12):16224-16242. doi: 10.1096/fj.202000768RR. Epub 2020 Oct 15. PMID: 33058343.
Kim H, Takegahara N, Walsh MC, Ueda J, Fujihara Y, Ikawa M, #Choi Y. Protocadherin-7 contributes to maintenance of bone homeostasis through regulation of osteoclast multinucleation. BMB Rep. 2020 Sep;53(9):472-477. PMID: 32635982. PMC7526982.
Robertson MJ, Kent K, Tharp N, Nozawa K, Dean L, Mathew M, Grimm SL, Yu Z, Légaré C, Fujihara Y, Ikawa M, Sullivan R, #Coarfa C, Matzuk MM, #Garcia TX. Large-scale discovery of male reproductive tract-specific genes through analysis of RNA-seq datasets. BMC Biol. 2020 Aug 19;18(1):103. doi: 10.1186/s12915-020-00826-z. PMID: 32814578. PMC7436996.
Lee GH, Fujita M, Nakanishi H, Miyata H, Ikawa M, Maeda Y, Murakami Y, #Kinoshita T. PGAP6, a GPI-specific phospholipase A2, has narrow substrate specificity against GPI-anchored proteins. J Biol Chem. 2020 Oct 16;295(42):14501-14509. doi: 10.1074/jbc.RA120.014643. Epub 2020 Aug 18. PMID: 32816994. PMC7573272.
Devlin DJ, Nozawa K, Ikawa M, #Matzuk MM. Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in micedagger. Biol Reprod. 2020 Aug 4;103(2):205-222. doi: 10.1093/biolre/ioaa082. PMID: 32588889. PMC7401401.
*Sun J, *Lu Y, Nozawa K, Xu Z, Morohoshi A, Castaneda JM, Noda T, Miyata H, Abbasi F, Shawki HH, Takahashi S, Devlin DJ, Yu Z, Matzuk RM, Garcia TX, #Martin MM, #Ikawa M. CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproductiondagger. Biol Reprod. 2020 Aug 4;103(2):183-194. doi: 10.1093/biolre/ioaa083. PMID: 32588039. PMC7401351.
*Park S, *Shimada K, Fujihara Y, Xu Z, Shimada K, Larasati T, Pratiwi P, Matzuk RM, Devlin DJ, Yu Z, Garcia TX, #Matzuk MM, #Ikawa M. CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity. Biol Reprod. 2020 Aug 4;103(2):195-204. doi: 10.1093/biolre/ioaa084. PMID: 32561905. PMC7401030.
*Larasati T, *Noda T, Fujihara Y, Shimada K, Tobita T, Yu Z, #Matzuk MM, #Ikawa M. Tmprss12 is required for sperm motility and uterotubal junction migration in micedagger. Biol Reprod. 2020 Aug 4;103(2):254-263. doi: 10.1093/biolre/ioaa060. PMID: 32529245. PMC7401031.
*Xu Z, *Miyata H, Kaneda Y, Castaneda JM, Lu Y, Morohoshi A, Yu Z, Matzuk MM, #Ikawa M. CIB4 is essential for the haploid phase of spermatogenesis in micedagger. Biol Reprod. 2020 Aug 4;103(2):235-243. doi: 10.1093/biolre/ioaa059. PMID: 32430498. PMC7401386.
Nozawa K, Zhang Q, Miyata H, Devlin DJ, Yu Z, Oura S, Koyano T, Matsuyama M, #Ikawa M, #Matzuk MM. Knockout of serine-rich single-pass membrane protein 1 (Ssmem1) causes globozoospermia and sterility in male micedagger. Biol Reprod. 2020 Aug 4;103(2):244-253. doi: 10.1093/biolre/ioaa040. PMID: 32301969. PMC7401026.
*Kobayashi K, *Endo T, Matsumura T, Lu Y, Yu Z, #Matzuk MM, #Ikawa M. Prss55 but not Prss51 is required for male fertility in micedagger. Biol Reprod. 2020 Aug 4;103(2):223-234. doi: 10.1093/biolre/ioaa041. PMID: 32301961. PMC7401375.
*Kim CR, *Noda T, Kim H, Kim G, Park S, Na Y, Oura S, Shimada K, Bang I, Ahn JY, Kim YR, Oh SK, Choi HJ, Kim JS, Jung I, Lee H, Okada Y, #Ikawa M, #Baek SH. PHF7 Modulates BRDT Stability and Histone-to-Protamine Exchange during Spermiogenesis. Cell Rep. 2020 Jul 28;32(4):107950. doi: 10.1016/j.celrep.2020.107950. PMID: 32726616.
#Bai J, Kondo R, Mayasari NI, Shigeoka T, Isotani A, Ikawa M, Sashida G, Kawaichi M, #Ishida Y. Diphtheria toxin-mediated transposon-driven poly (A)-trapping efficiently disrupts transcriptionally silent genes in embryonic stem cells. Genesis. 2020 Sep;58(9):e23386. doi: 10.1002/dvg.23386. Epub 2020 Jul 9. PMID: 32645254.
Senoo M, Hozoji H, Ishikawa-Yamauchi Y, Takijiri T, Ohta S, Ukai T, Kabata M, Yamamoto T, Yamada Y, #Ikawa M, #Ozawa M. RNA-binding protein Ptbp1 regulates alternative splicing and transcriptome in spermatogonia and maintains spermatogenesis in concert with Nanos3. J Reprod Dev. 2020 Oct 13;66(5):459-467. doi: 10.1262/jrd.2020-060. Epub 2020 Jul 6. PMID: 32624547. PMC7593632.
*Liu C, *Miyata H, *Gao Y, *Sha Y, *Tang S, *Xu Z, Whitfield M, Patrat C, Wu H, Dulioust E, Tian S, Shimada K, Cong J, Noda T, Li H, Morohoshi A, Cazin C, Kherraf ZE, Arnoult C, Jin L, He X, Ray PF, Cao Y, Touré A, #Zhang F, #Ikawa M. Bi-allelic DNAH8 Variants Lead to Multiple Morphological Abnormalities of the Sperm Flagella and Primary Male Infertility. Am J Hum Genet. 2020 Aug 6;107(2):330-341. doi: 10.1016/j.ajhg.2020.06.004. Epub 2020 Jul 2. PMID: 32619401. PMC7413861.
Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren MK, Horie M, Okabe M, #Matzuk MM, #Ikawa M. NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility. Science. 2020 Jun 5;368(6495):1132-1135. doi: 10.1126/science.aay5134. PMID: 32499443. PMC7396227.
*#Kojima M, Otabi H, Kumanogoh H, Toyoda A, Ikawa M, Okabe M, *#Mizui T. Reduction in BDNF from Inefficient Precursor Conversion Influences Nest Building and Promotes Depressive-Like Behavior in Mice. Int J Mol Sci. 2020 Jun 1;21(11). pii: ijms21113984. doi: 10.3390/ijms21113984. PMID: 32492978. PMC7312902.
*Noda T, *Lu Y, Fujihara Y, Oura S, Koyano T, Kobayashi S, Matzuk MM, #Ikawa M. Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm-oocyte fusion in mice. Proc Natl Acad Sci U S A. 2020 May 26;117(21):11493-11502. doi: 10.1073/pnas.1922650117. Epub 2020 May 11. PMID: 32393636. PMC7261011.
Fujihara Y, Lu Y, Noda T, Oji A, Larasati T, Kojima-Kita K, Yu Z, Matzuk RM, #Matzuk MM, #Ikawa M. Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice. Proc Natl Acad Sci U S A. 2020 Apr 28;117(17):9393-9400. doi: 10.1073/pnas.1917060117. Epub 2020 Apr 15. PMID: 32295885. PMC7196805.
Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, #Ikawa M. Tesmin, Metallothionein-Like 5, is Required for Spermatogenesis in Micedagger. Biol Reprod. 2020 Apr 15;102(4):975-983. doi: 10.1093/biolre/ioaa002. PMID: 31916570. PMC7124961.
#Castaneda JM, Miyata H, Archambeault DR, Satouh Y, Yu Z, Ikawa M, Matzuk MM. Mouse t-complex protein 11 is important for progressive motility in spermdagger. Biol Reprod. 2020 Apr 15;102(4):852-862. doi: 10.1093/biolre/ioz226. PMID: 31837139. PMC7124965.
*Umeda R, *Satouh Y, Takemoto M, Nakada-Nakura Y, Liu K, Yokoyama T, Shirouzu M, Iwata S, Nomura N, Sato K, Ikawa M, #Nishizawa T, #Nureki O. Structural insights into tetraspanin CD9 function. Nat Commun. 2020 Mar 30;11(1):1606. doi: 10.1038/s41467-020-15459-7. PMID: 32231207. PMC7105497.
*Abbasi F, *Kodani M, Emori C, Kiyozumi D, Mori M, Fujihara Y, #Ikawa M. CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice. Cells. 2020 Mar 28;9(4). pii: cells9040821. doi: 10.3390/cells9040821. PMID: 32231122. PMC7226750.
Shimizu A, Zankov DP, Sato A, Komeno M, Toyoda F, Yamazaki S, Makita T, Noda T, Ikawa M, Asano Y, Miyashita Y, Takashima S, Morita H, Ishikawa T, Makita N, Hitosugi M, Matsuura H, Ohno S, Horie M, #Ogita H. Identification of transmembrane protein 168 mutation in familial Brugada syndrome. FASEB J. 2020 May;34(5):6399-6417. doi: 10.1096/fj.201902991R. Epub 2020 Mar 16. PMID: 32175648.
Tsumagari R, Kakizawa S, Kikunaga S, Fujihara Y, Ueda S, Yamanoue M, Saito N, Ikawa M, #Shirai Y. DGKgamma Knock-Out Mice Show Impairments in Cerebellar Motor Coordination, LTD, and the Dendritic Development of Purkinje Cells through the Activation of PKCgamma. eNeuro. 2020 Mar 4;7(2). pii: ENEURO.0319-19.2020. doi: 10.1523/ENEURO.0319-19.2020. Print 2020 Mar/Apr. PMID: 32033984. PMC7057140.
#Miyata H, Shimada K, Morohoshi A, Oura S, Matsumura T, Xu Z, Oyama Y, #Ikawa M. Testis-enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa. FASEB J. 2020 Apr;34(4):5389-5400. doi: 10.1096/fj.201902755R. Epub 2020 Feb 19. PMID: 32072696. PMC7136151.
Holcomb RJ, Oura S, Nozawa K, Kent K, Yu Z, Robertson MJ, Coarfa C, Matzuk MM, Ikawa M, #Garcia TX. The testis-specific serine proteases PRSS44, PRSS46, and PRSS54 are dispensable for male mouse fertilitydagger. Biol Reprod. 2020 Feb 12;102(1):84-91. doi: 10.1093/biolre/ioz158. PMID: 31403672. PMC7013879.
*Kim H, Takegahara N, Walsh MC, Middleton SA, Yu J, Shirakawa J, Ueda J, Fujihara Y, Ikawa M, Ishii M, Kim J, #Choi Y. IgSF11 regulates osteoclast differentiation through association with the scaffold protein PSD-95. Bone Res. 2020 Feb 10;8:5. doi: 10.1038/s41413-019-0080-9. eCollection 2020. PMID: 32047704. PMC7010662.
Morohoshi A, Miyata H, Shimada K, Nozawa K, Matsumura T, Yanase R, Shiba K, Inaba K, #Ikawa M. Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice. PLoS Genet. 2020 Jan 21;16(1):e1008585. doi: 10.1371/journal.pgen.1008585. eCollection 2020 Jan. PMID: 31961863. PMC6994161.
*Kondo H, *Matsumura T, Kaneko M, Inoue K, Kosako H, Ikawa M, Takahama Y, #Ohigashi I. PITHD1 is a proteasome-interacting protein essential for male fertilization. J Biol Chem. 2020 Feb 7;295(6):1658-1672. doi: 10.1074/jbc.RA119.011144. Epub 2020 Jan 8. PMID: 31915251. PMC7008373.
Nakashima A, Cheng SB, Ikawa M, Yoshimori T, Huber WJ, Menon R, Huang Z, Fierce J, Padbury JF, Sadovsky Y, Saito S, #Sharma S. Evidence for lysosomal biogenesis proteome defect and impaired autophagy in preeclampsia. Autophagy. 2020 Oct;16(10):1771-1785. doi: 10.1080/15548627.2019.1707494. Epub 2019 Dec 26. PMID: 31856641. PMC8386603.
Khattar E, Maung KZY, Chew CL, Ghosh A, Mok MMH, Lee P, Zhang J, Chor WHJ, Cildir G, Wang CQ, Mohd-Ismail NK, Chin DWL, Lee SC, Yang H, Shin YJ, Nam DH, Chen L, Kumar AP, Deng LW, Ikawa M, Gunaratne J, Osato M, #Tergaonkar V. Rap1 regulates hematopoietic stem cell survival and affects oncogenesis and response to chemotherapy. Nat Commun. 2019 Dec 13;10(1):5349. doi: 10.1038/s41467-019-13082-9. PMID: 31836706. PMC6911077.
Kawai T, Miyata H, Nakanishi H, Sakata S, Morioka S, Sasaki J, Watanabe M, Sakimura K, Fujimoto T, Sasaki T, Ikawa M, #Okamura Y. Polarized PtdIns(4,5)P2 distribution mediated by a voltage-sensing phosphatase (VSP) regulates sperm motility. Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):26020-26028. doi: 10.1073/pnas.1916867116. Epub 2019 Nov 27. PMID: 31776261. PMC6925991.
#Serizawa T, Isotani A, Matsumura T, Nakanishi K, Nonaka S, Shibata S, Ikawa M, #Okano H. Developmental analyses of mouse embryos and adults using a non- overlapping tracing system for all three germ layers. Development. 2019 Nov 4;146(21). pii: dev.174938. doi: 10.1242/dev.174938. PMID: 31597657.
#Kiyozumi D, Mori M, Kodani M, #Ikawa M. Genetic mutation of Frem3 does not causeFraser syndrome in mice. Exp Anim. 2020 Jan 29;69(1):104-109. doi: 10.1538/expanim.19-0088. Epub 2019 Sep 26. PMID: 31554749. PMC7004800.
*Matsumura T, *Noda T, Muratani M, Okada R, Yamane M, Isotani A, Kudo T, Takahashi S, #Ikawa M. Male mice, caged in the International Space Station for 35 days, sire healthy offspring. Sci Rep. 2019 Sep 24;9(1):13733. doi: 10.1038/s41598-019-50128-w. PMID: 31551430. PMC6760203.
*Fujihara Y, *Noda T, *Kobayashi K, Oji A, Kobayashi S, Matsumura T, Larasati T, Oura S, Kojima-Kita K, Yu Z, #Matzuk MM, #Ikawa M. Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice. Proc Natl Acad Sci U S A. 2019 Sep 10;116(37):18498-18506. doi: 10.1073/pnas.1908736116. Epub 2019 Aug 27. PMID: 31455729. PMC6744855.
Hayashi M, #Machida T, Ishida Y, Ogata Y, Omori T, Takasumi M, Endo Y, Suzuki T, Sekimata M, Homma Y, Ikawa M, Ohira H, Fujita T, Sekine H. Cutting Edge: Role of MASP-3 in the Physiological Activation of Factor D of the Alternative Complement Pathway. J Immunol. 2019 Sep 15;203(6):1411-1416. doi: 10.4049/jimmunol.1900605. Epub 2019 Aug 9. PMID: 31399515.
Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, Shimada K, Miyata H, Tobita T, Noda T, Castaneda JM, Kiyozumi D, Zhang Q, Larasati T, Young SAM, Kodani M, Huddleston CA, Robertson MJ, Coarfa C, Isotani A, Aitken RJ, Okabe M, Matzuk MM, Garcia TX, #Ikawa M. CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in micedagger. Biol Reprod. 2019 Aug 1;101(2):501-511. doi: 10.1093/biolre/ioz103. PMID: 31201419. PMC6735960.
Shiromoto Y, #Kuramochi-Miyagawa S, Nagamori I, Chuma S, Arakawa T, Nishimura T, Hasuwa H, Tachibana T, Ikawa M, #Nakano T. GPAT2 is required for piRNA biogenesis, transposon silencing, and maintenance of spermatogonia in micedagger. Biol Reprod. 2019 Jul 1;101(1):248-256. doi: 10.1093/biolre/ioz056. PMID: 30951587.
Sasaki K, Shiba K, Nakamura A, Kawano N, Satouh Y, Yamaguchi H, Morikawa M, Shibata D, Yanase R, Jokura K, Nomura M, Miyado M, Takada S, Ueno H, Nonaka S, Baba T, Ikawa M, Kikkawa M, Miyado K, #Inaba K. Calaxin is required for cilia-driven determination of vertebrate laterality. Commun Biol. 2019 Jun 20;2:226. doi: 10.1038/s42003-019-0462-y. eCollection 2019. PMID: 31240264. PMC6586612.
Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, #Ikawa M. Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecunditydagger. Biol Reprod. 2019 Apr 1;100(4):1035-1045. doi: 10.1093/biolre/ioy247. PMID: 30452524. PMC6483057.
*Noda T, *Sakurai N, Nozawa K, Kobayashi S, Devlin DJ, #Matzuk MM, #Ikawa M. Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice. Andrology. 2019 Sep;7(5):644-653. doi: 10.1111/andr.12621. Epub 2019 Mar 29. PMID: 30927342. PMC6688925.
Shide K, Kameda T, Kamiunten A, Oji A, Ozono Y, Sekine M, Honda A, Kitanaka A, Akizuki K, Tahira Y, Nakamura K, Hidaka T, Kubuki Y, Abe H, Miike T, Iwakiri H, Tahara Y, Sueta M, Hasuike S, Yamamoto S, Nagata K, Ikawa M, #Shimoda K. Mice with Calr mutations homologous to human CALR mutations only exhibit mild thrombocytosis. Blood Cancer J. 2019 Mar 29;9(4):42. doi: 10.1038/s41408-019-0202-z. PMID: 30926777. PMC6440999.
Kusakabe S, Suzuki T, Sugiyama Y, Haga S, Horike K, Tokunaga M, Hirano J, Zhang H, Chen DV, Ishiga H, Komoda Y, Ono C, Fukuhara T, Yamamoto M, Ikawa M, Satoh T, Akira S, Tanaka T, Moriishi K, Fukai M, Taketomi A, Yoshio S, Kanto T, Suzuki T, Okamoto T, #Matsuura Y. USP15 Participates in Hepatitis C Virus Propagation through Regulation of Viral RNA Translation and Lipid Droplet Formation. J Virol. 2019 Mar 5;93(6). pii: JVI.01708-18. doi: 10.1128/JVI.01708-18. Print 2019 Mar 15. PMID: 30626683. PMC6401473.
Tobita T, Kiyozumi D, Muto M, Noda T, #Ikawa M. Lvrn expression is not critical for mouse placentation. J Reprod Dev. 2019 Jun 14;65(3):239-244. doi: 10.1262/jrd.2018-157. Epub 2019 Feb 10. PMID: 30745494. PMC6584185.
Shimada K, Kato H, Miyata H, #Ikawa M. Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis. J Reprod Dev. 2019 Apr 12;65(2):155-162. doi: 10.1262/jrd.2018-136. Epub 2019 Jan 21. PMID: 30662012. PMC6473107.
Matsumura T, Endo T, Isotani A, Ogawa M, #Ikawa M. An azoospermic factor gene, Ddx3y and its paralog, Ddx3x are dispensable in germ cells for male fertility. J Reprod Dev. 2019 Apr 12;65(2):121-128. doi: 10.1262/jrd.2018-145. Epub 2019 Jan 7. PMID: 30613052. PMC6473106.
Kimura H, Yamamoto H, Harada T, Fumoto K, Osugi Y, Sada R, Maehara N, Hikita H, Mori S, Eguchi H, Ikawa M, Takehara T, #Kikuchi A. CKAP4, a DKK1 Receptor, Is a Biomarker in Exosomes Derived from Pancreatic Cancer and a Molecular Target for Therapy. Clin Cancer Res. 2019 Mar 15;25(6):1936-1947. doi: 10.1158/1078-0432.CCR-18-2124. Epub 2019 Jan 4. PMID: 30610103.
Carvajal G, Brukman NG, Weigel Munoz M, Battistone MA, Guazzone VA, Ikawa M, Haruhiko M, Lustig L, Breton S, #Cuasnicu PS. Impaired male fertility and abnormal epididymal epithelium differentiation in mice lacking CRISP1 and CRISP4. Sci Rep. 2018 Dec 3;8(1):17531. doi: 10.1038/s41598-018-35719-3. PMID: 30510210. PMC6277452.
Senoo M, Takijiri T, Yoshida N, Ozawa M, #Ikawa M. PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia. J Reprod Dev. 2019 Feb 8;65(1):37-46. doi: 10.1262/jrd.2018-109. Epub 2018 Nov 12. PMID: 30416150. PMC6379764.
Sato Y, Kiyozumi D, Futaki S, Nakano I, Shimono C, Kaneko N, Ikawa M, Okabe M, Sawamoto K, #Sekiguchi K. Ventricular-subventricular zone fractones are speckled basement membranes that function as a neural stem cell niche. Mol Biol Cell. 2019 Jan 1;30(1):56-68. doi: 10.1091/mbc.E18-05-0286. Epub 2018 Oct 31. PMID: 30379609. PMC6337917.
*Abbasi F, *Miyata H, Shimada K, Morohoshi A, Nozawa K, Matsumura T, Xu Z, Pratiwi P, #Ikawa M. RSPH6A is required for sperm flagellum formation and male fertility in mice. J Cell Sci. 2018 Oct 11;131(19). pii: jcs.221648. doi: 10.1242/jcs.221648. PMID: 30185526. PMC6198453.
Fujihara Y, Oji A, Kojima-Kita K, Larasati T, #Ikawa M. Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice. J Cell Sci. 2018 Oct 8;131(19). pii: jcs.221481. doi: 10.1242/jcs.221481. PMID: 30209135. PMC6198454.
Suzuki T, #Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, Fukuhara T, Ikawa M, Satoh T, Yoshio S, Suzuki R, Saijo M, Huang DCS, Kanto T, Akira S, #Matsuura Y. Infection with flaviviruses requires BCLXL for cell survival. PLoS Pathog. 2018 Sep 27;14(9):e1007299. doi: 10.1371/journal.ppat.1007299. eCollection 2018 Sep. PMID: 30261081. PMC6177207.
*Sakamoto S, *#Thumkeo D, Ohta H, Zhang Z, Huang S, Kanchanawong P, Fuu T, Watanabe S, Shimada K, Fujihara Y, Yoshida S, Ikawa M, Watanabe N, Saitou M, #Narumiya S. mDia1/3 generate cortical F-actin meshwork in Sertoli cells that is continuous with contractile F-actin bundles and indispensable for spermatogenesis and male fertility. PLoS Biol. 2018 Sep 26;16(9):e2004874. doi: 10.1371/journal.pbio.2004874. eCollection 2018 Sep. PMID: 30256801. PMC6175529.
Kiyozumi D, Taniguchi Y, Nakano I, Toga J, Yagi E, Hasuwa H, Ikawa M, #Sekiguchi K. Laminin gamma1 C-terminal Glu to Gln mutation induces early postimplantation lethality. Life Sci Alliance. 2018 Sep 10;1(5):e201800064. doi: 10.26508/lsa.201800064. eCollection 2018 Oct. PMID: 30456378. PMC6238537.
#Nishimasu H, Shi X, Ishiguro S, Gao L, Hirano S, Okazaki S, Noda T, Abudayyeh OO, Gootenberg JS, Mori H, Oura S, Holmes B, Tanaka M, Seki M, Hirano H, Aburatani H, Ishitani R, Ikawa M, Yachie N, Zhang F, #Nureki O. Engineered CRISPR-Cas9 nuclease with expanded targeting space. Science. 2018 Sep 21;361(6408):1259-1262. doi: 10.1126/science.aas9129. Epub 2018 Aug 30. PMID: 30166441. PMC6368452.
Aoki A, Nakashima A, Kusabiraki T, Ono Y, Yoshino O, Muto M, Kumasawa K, Yoshimori T, #Ikawa M, Saito S. Trophoblast-Specific Conditional Atg7 Knockout Mice Develop Gestational Hypertension. Am J Pathol. 2018 Nov;188(11):2474-2486. doi: 10.1016/j.ajpath.2018.07.021. Epub 2018 Aug 27. PMID: 30165042.
Oura S, Miyata H, Noda T, Shimada K, Matsumura T, Morohoshi A, Isotani A, #Ikawa M. Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice. Exp Anim. 2019 Feb 26;68(1):25-34. doi: 10.1538/expanim.18-0071. Epub 2018 Aug 9. PMID: 30089752. PMC6389518.
#Fustin JM, Kojima R, Itoh K, Chang HY, Ye S, Zhuang B, Oji A, Gibo S, Narasimamurthy R, Virshup D, Kurosawa G, Doi M, Manabe I, Ishihama Y, Ikawa M, #Okamura H. Two Ck1delta transcripts regulated by m6A methylation code for two antagonistic kinases in the control of the circadian clock. Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):5980-5985. doi: 10.1073/pnas.1721371115. Epub 2018 May 21. PMID: 29784786. PMC6003373.
#Tanaka T, Shimizu S, Ueno M, Fujihara Y, Ikawa M, Miyata S. MARCKSL1 Regulates Spine Formation in the Amygdala and Controls the Hypothalamic-Pituitary-Adrenal Axis and Anxiety-Like Behaviors. EBioMedicine. 2018 Apr;30:62-73. doi: 10.1016/j.ebiom.2018.03.018. Epub 2018 Mar 19. PMID: 29580842. PMC5952351.
Murase M, #Kawasaki T, Hakozaki R, Sueyoshi T, Putri DDP, Kitai Y, Sato S, Ikawa M, #Kawai T. Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking. J Immunol. 2018 Apr 15;200(8):2798-2808. doi: 10.4049/jimmunol.1701390. Epub 2018 Mar 14. PMID: 29540576.
Shibata T, Shibata S, Ishigaki Y, Kiyokawa E, Ikawa M, Singh DP, Sasaki H, #Kubo E. Tropomyosin 2 heterozygous knockout in mice using CRISPR-Cas9 system displays the inhibition of injury-induced epithelial-mesenchymal transition, and lens opacity. Mech Ageing Dev. 2018 Apr;171:24-30. doi: 10.1016/j.mad.2018.03.001. Epub 2018 Mar 3. PMID: 29510160. PMC6013838.
Fujita T, Kitaura F, Oji A, Tanigawa N, Yuno M, Ikawa M, Taniuchi I, #Fujii H. Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis. Genes Cells. 2018 Apr;23(4):318-325. doi: 10.1111/gtc.12573. Epub 2018 Feb 26. PMID: 29480524.
Nozawa K, Satouh Y, Fujimoto T, Oji A, #Ikawa M. Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice. Sci Rep. 2018 Jan 22;8(1):1315. doi: 10.1038/s41598-018-19497-6. PMID: 29358633. PMC5778054.
Abe S, Kobayashi K, Oji A, Sakuma T, Kazuki K, Takehara S, Nakamura K, Okada A, Tsukazaki Y, Senda N, Honma K, Yamamoto T, Ikawa M, Chiba K, Oshimura M, #Kazuki Y. Modification of single-nucleotide polymorphism in a fully humanized CYP3A mouse by genome editing technology. Sci Rep. 2017 Nov 9;7(1):15189. doi: 10.1038/s41598-017-15033-0. PMID: 29123154. PMC5680201.
Fujihara Y, Oji A, Larasati T, Kojima-Kita K, #Ikawa M. Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models. Int J Mol Sci. 2017 Oct 21;18(10). pii: ijms18102208. doi: 10.3390/ijms18102208. PMID: 29065458. PMC5666888.
*Mashiko D, Ikawa M, #Fujimoto K. Mouse spermatozoa with higher fertilization rates have thinner nuclei. PeerJ. 2017 Oct 12;5:e3913. doi: 10.7717/peerj.3913. eCollection 2017. PMID: 29038763. PMC5641427.
Honda M, Hidaka K, Fukada SI, Sugawa R, Shirai M, Ikawa M, #Morisaki T. Vestigial-like 2 contributes to normal muscle fiber type distribution in mice. Sci Rep. 2017 Aug 2;7(1):7168. doi: 10.1038/s41598-017-07149-0. PMID: 28769032. PMC5540913.
Kurihara T, #Fukuhara T, Ono C, Yamamoto S, Uemura K, Okamoto T, Sugiyama M, Motooka D, Nakamura S, Ikawa M, Mizokami M, Maehara Y, #Matsuura Y. Suppression of HBV replication by the expression of nickase- and nuclease dead-Cas9. Sci Rep. 2017 Jul 21;7(1):6122. doi: 10.1038/s41598-017-05905-w. PMID: 28733609. PMC5522428.
#Isotani A, Matsumura T, Ogawa M, Tanaka T, Yamagata K, Ikawa M, Okabe M. A delayed sperm penetration of cumulus layers by disruption of acrosin gene in rats. Biol Reprod. 2017 Jul 1;97(1):61-68. doi: 10.1093/biolre/iox066. PMID: 28859281.
Castaneda JM, Hua R, Miyata H, Oji A, Guo Y, Cheng Y, Zhou T, Guo X, Cui Y, Shen B, Wang Z, Hu Z, Zhou Z, Sha J, Prunskaite-Hyyrylainen R, Yu Z, Ramirez-Solis R, #Ikawa M, #Matzuk MM, #Liu M. TCTE1 is a conserved component of the dynein regulatory complex and is required for motility and metabolism in mouse spermatozoa. Proc Natl Acad Sci U S A. 2017 Jul 3;114(27):E5370-E5378. doi: 10.1073/pnas.1621279114. Epub 2017 Jun 19. PMID: 28630322. PMC5502601.
Kitada S, Kayama H, Okuzaki D, Koga R, Kobayashi M, Arima Y, Kumanogoh A, Murakami M, Ikawa M, #Takeda K. BATF2 inhibits immunopathological Th17 responses by suppressing Il23a expression during Trypanosoma cruzi infection. J Exp Med. 2017 May 1;214(5):1313-1331. doi: 10.1084/jem.20161076. Epub 2017 Mar 29. PMID: 28356392. PMC5413328.
#Ishimoto K, Ikawa M, #Okabe M. The mechanics clarifying counterclockwise rotation in most IVF eggs in mice. Sci Rep. 2017 Mar 3;7:43456. doi: 10.1038/srep43456. PMID: 28256541. PMC5335560.
Satouh Y, Nozawa K, Yamagata K, Fujimoto T, #Ikawa M. Viable offspring after imaging of Ca2+ oscillations and visualization of the cortical reaction in mouse eggs. Biol Reprod. 2017 Mar 1;96(3):563-575. doi: 10.1093/biolre/iox002. PMID: 28339615.
Naruse C, Shibata S, Tamura M, Kawaguchi T, Abe K, Sugihara K, Kato T, Nishiuchi T, Wakana S, Ikawa M, #Asano M. New insights into the role of Jmjd3 and Utx in axial skeletal formation in mice. FASEB J. 2017 Jun;31(6):2252-2266. doi: 10.1096/fj.201600642R. Epub 2017 Feb 10. PMID: 28188179.
Onda K, #Tong S, Beard S, Binder N, Muto M, Senadheera SN, Parry L, Dilworth M, Renshall L, Brownfoot F, Hastie R, Tuohey L, Palmer K, Hirano T, Ikawa M, Kaitu'u-Lino T, Hannan NJ. Proton Pump Inhibitors Decrease Soluble fms-Like Tyrosine Kinase-1 and Soluble Endoglin Secretion, Decrease Hypertension, and Rescue Endothelial Dysfunction. Hypertension. 2017 Mar;69(3):457-468. doi: 10.1161/HYPERTENSIONAHA.116.08408. Epub 2017 Jan 23. PMID: 28115513.
#Ueda J, Harada A, Urahama T, Machida S, Maehara K, Hada M, Makino Y, Nogami J, Horikoshi N, Osakabe A, Taguchi H, Tanaka H, Tachiwana H, Yao T, Yamada M, Iwamoto T, Isotani A, Ikawa M, Tachibana T, Okada Y, Kimura H, Ohkawa Y, Kurumizaka H, #Yamagata K. Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis. Cell Rep. 2017 Jan 17;18(3):593-600. doi: 10.1016/j.celrep.2016.12.065. PMID: 28099840.
Ishikawa T, #Kitaura Y, Kadota Y, Morishita Y, Ota M, Yamanaka F, Xu M, Ikawa M, Inoue N, Kawano F, Nakai N, Murakami T, Miura S, Hatazawa Y, Kamei Y, #Shimomura Y. Muscle-specific deletion of BDK amplifies loss of myofibrillar protein during protein undernutrition. Sci Rep. 2017 Jan 4;7:39825. doi: 10.1038/srep39825. PMID: 28051178. PMC5209746.
Lee GH, Fujita M, Takaoka K, Murakami Y, Fujihara Y, Kanzawa N, Murakami KI, Kajikawa E, Takada Y, Saito K, Ikawa M, Hamada H, Maeda Y, #Kinoshita T. A GPI processing phospholipase A2, PGAP6, modulates Nodal signaling in embryos by shedding CRIPTO. J Cell Biol. 2016 Dec 5;215(5):705-718. doi: 10.1083/jcb.201605121. Epub 2016 Nov 23. PMID: 27881714. PMC5147002.
Young SA, Miyata H, Satouh Y, Aitken RJ, Baker MA, #Ikawa M. CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa. J Cell Sci. 2016 Dec 1;129(23):4379-4387. doi: 10.1242/jcs.193151. Epub 2016 Oct 19. PMID: 27802166.
Young SA, Miyata H, Satouh Y, Muto M, Larsen MR, Aitken RJ, Baker MA, #Ikawa M. CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility. Reproduction. 2016 Dec;152(6):665-672. doi: 10.1530/REP-16-0150. Epub 2016 Sep 13. PMID: 27624483.
Takashima K, Takeda Y, Oshiumi H, Shime H, Okabe M, Ikawa M, Matsumoto M, #Seya T. STING in tumor and host cells cooperatively work for NK cell-mediated tumor growth retardation. Biochem Biophys Res Commun. 2016 Sep 30;478(4):1764-71. doi: 10.1016/j.bbrc.2016.09.021. Epub 2016 Sep 5. PMID: 27608599.
Oji A, Noda T, Fujihara Y, Miyata H, Kim YJ, Muto M, Nozawa K, Matsumura T, Isotani A, #Ikawa M. CRISPR/Cas9 mediated genome editing in ES cells and its application for chimeric analysis in mice. Sci Rep. 2016 Aug 17;6:31666. doi: 10.1038/srep31666. PMID: 27530713. PMC4987700.
Kato K, Satouh Y, Nishimasu H, Kurabayashi A, Morita J, Fujihara Y, Oji A, Ishitani R, *Ikawa M, #Nureki O. Structural and functional insights into IZUMO1 recognition by JUNO in mammalian fertilization. Nat Commun. 2016 Jul 15;7:12198. doi: 10.1038/ncomms12198. PMID: 27416963. PMC4947182.
#Shimada M, Goshima T, Matsuo H, Johmura Y, Haruta M, Murata K, Tanaka H, Ikawa M, Nakanishi K, #Nakanishi M. Essential role of autoactivation circuitry on Aurora B-mediated H2AX- pS121 in mitosis. Nat Commun. 2016 Jul 8;7:12059. doi: 10.1038/ncomms12059. PMID: 27389782. PMC4941122.
Fujimoto K, Kinoshita M, Tanaka H, Okuzaki D, Shimada Y, Kayama H, Okumura R, Furuta Y, Narazaki M, Tamura A, Hatakeyama S, Ikawa M, Tsuchiya K, Watanabe M, Kumanogoh A, Tsukita S, #Takeda K. Regulation of intestinal homeostasis by the ulcerative colitis-associated gene RNF186. Mucosal Immunol. 2017 Mar;10(2):446-459. doi: 10.1038/mi.2016.58. Epub 2016 Jul 6. PMID: 27381925.
Miyata H, Castaneda JM, Fujihara Y, Yu Z, Archambeault DR, Isotani A, Kiyozumi D, Kriseman ML, Mashiko D, Matsumura T, Matzuk RM, Mori M, Noda T, Oji A, Okabe M, Prunskaite-Hyyrylainen R, Ramirez-Solis R, Satouh Y, Zhang Q, #Ikawa M, #Matzuk MM. Genome engineering uncovers 54 evolutionarily conserved and testis- enriched genes that are not required for male fertility in mice. Proc Natl Acad Sci U S A. 2016 Jul 12;113(28):7704-10. doi: 10.1073/pnas.1608458113. Epub 2016 Jun 29. PMID: 27357688. PMC4948324.
Kanemori Y, Koga Y, Sudo M, Kang W, Kashiwabara S, Ikawa M, Hasuwa H, Nagashima K, Ishikawa Y, Ogonuki N, Ogura A, #Baba T. Biogenesis of sperm acrosome is regulated by pre-mRNA alternative splicing of Acrbp in the mouse. Proc Natl Acad Sci U S A. 2016 Jun 28;113(26):E3696-705. doi: 10.1073/pnas.1522333113. Epub 2016 Jun 14. PMID: 27303034. PMC4932935.
Yamazaki D, Funato Y, Miyata H, Ikawa M, #Miki H. Complementary role of CNNM2 in sperm motility and Ca(2+) influx during capacitation. Biochem Biophys Res Commun. 2016 Jun 3;474(3):441-446. doi: 10.1016/j.bbrc.2016.05.001. Epub 2016 May 2. PMID: 27150626.
#Matsuura R, Maeda A, Sakai R, Eguchi H, Lo PC, Hasuwa H, Ikawa M, Nakahata K, Zenitani M, Yamamichi T, Umeda S, Deguchi K, Okuyama H, Miyagawa S. Human HLA-Ev (147) Expression in Transgenic Animals. Transplant Proc. 2016 May;48(4):1323-5. doi: 10.1016/j.transproceed.2015.10.069. PMID: 27320614.
#Sakai R, Esaki Y, Hasuwa H, Ikawa M, Lo P, Matsuura R, Nakahata K, Zenitani M, Asada M, Maeda A, Eguchi H, Okuyama H, Miyagawa S. Knockout of Cytidine Monophospho-N-Acetylneuraminic Acid (CMP-NeuAc) Hydroxylase From Porcine Endothelial Cells by a CRISPR System. Transplant Proc. 2016 May;48(4):1320-2. doi: 10.1016/j.transproceed.2015.10.065. PMID: 27320613.
#Sakai R, Maeda A, Matsuura R, Eguchi H, Lo P, Hasuwa H, Ikawa M, Nakahata K, Zenitani M, Yamamichi T, Umeda S, Okuyama H, Miyagawa S. Expression of a Synthetic Gene of CTDM by Transgenic Animals. Transplant Proc. 2016 May;48(4):1279-81. doi: 10.1016/j.transproceed.2015.10.067. PMID: 27320603.
#Isotani A, Yamagata K, Okabe M, Ikawa M. Generation of Hprt-disrupted rat through mouse<--rat ES chimeras. Sci Rep. 2016 Apr 11;6:24215. doi: 10.1038/srep24215. PMID: 27062982. PMC4827079.
Kai T, Tsukamoto Y, Hijiya N, Tokunaga A, Nakada C, Uchida T, Daa T, Iha H, Takahashi M, Nomura T, Sato F, Mimata H, Ikawa M, Seto M, #Matsuura K, #Moriyama M. Kidney-specific knockout of Sav1 in the mouse promotes hyperproliferation of renal tubular epithelium through suppression of the Hippo pathway. J Pathol. 2016 May;239(1):97-108. doi: 10.1002/path.4706. Epub 2016 Mar 30. PMID: 26913567.
Yamazaki D, Miyata H, Funato Y, Fujihara Y, Ikawa M, #Miki H. The Mg2+ transporter CNNM4 regulates sperm Ca2+ homeostasis and is essential for reproduction. J Cell Sci. 2016 May 1;129(9):1940-9. doi: 10.1242/jcs.182220. Epub 2016 Mar 22. PMID: 27006114.
Muro Y, Hasuwa H, Isotani A, Miyata H, Yamagata K, Ikawa M, Yanagimachi R, #Okabe M. Behavior of Mouse Spermatozoa in the Female Reproductive Tract from Soon after Mating to the Beginning of Fertilization. Biol Reprod. 2016 Apr;94(4):80. doi: 10.1095/biolreprod.115.135368. Epub 2016 Mar 9. PMID: 26962112.
Zimmerman KA, Xing D, Pallero MA, Lu A, Ikawa M, Black L, Hoyt KL, Kabarowski JH, Michalak M, #Murphy-Ullrich JE. Calreticulin Regulates Neointima Formation and Collagen Deposition following Carotid Artery Ligation. J Vasc Res. 2015;52(5):306-20. doi: 10.1159/000443884. Epub 2016 Feb 25. PMID: 26910059. PMC4816666.
Shimbo M, #Kudo T, Hamada M, Jeon H, Imamura Y, Asano K, Okada R, Tsunakawa Y, Mizuno S, Yagami K, Ishikawa C, Li H, Shiga T, Ishida J, Hamada J, Murata K, Ishimaru T, Hashimoto M, Fukamizu A, Yamane M, Ikawa M, Morita H, Shinohara M, Asahara H, Akiyama T, Akiyama N, Sasanuma H, Yoshida N, Zhou R, Wang YY, Ito T, Kokubu Y, Noguchi TA, Ishimine H, Kurisaki A, Shiba D, Mizuno H, Shirakawa M, Ito N, Takeda S, Takahashi S. Ground-based assessment of JAXA mouse habitat cage unit by mouse phenotypic studies. Exp Anim. 2016 May 20;65(2):175-87. doi: 10.1538/expanim.15-0077. Epub 2016 Jan 28. PMID: 26822934. PMC4873486.
Brukman NG, Miyata H, Torres P, Lombardo D, Caramelo JJ, Ikawa M, Da Ros VG, #Cuasnicu PS. Fertilization defects in sperm from Cysteine-rich secretory protein 2 (Crisp2) knockout mice: implications for fertility disorders. Mol Hum Reprod. 2016 Apr;22(4):240-51. doi: 10.1093/molehr/gaw005. Epub 2016 Jan 19. PMID: 26786179.
Gyobu S, Miyata H, Ikawa M, Yamazaki D, Takeshima H, Suzuki J, #Nagata S. A Role of TMEM16E Carrying a Scrambling Domain in Sperm Motility. Mol Cell Biol. 2015 Dec 14;36(4):645-59. doi: 10.1128/MCB.00919-15. Print 2016 Feb 15. PMID: 26667038. PMC4751691.
Muto M, Fujihara Y, Tobita T, Kiyozumi D, #Ikawa M. Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice. Biol Reprod. 2016 Jan;94(1):6. doi: 10.1095/biolreprod.115.133454. Epub 2015 Nov 19. PMID: 26586843.
Latos PA, Sienerth AR, Murray A, Senner CE, Muto M, Ikawa M, Oxley D, Burge S, #Cox BJ, #Hemberger M. Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks. Genes Dev. 2015 Dec 1;29(23):2435-48. doi: 10.1101/gad.268821.115. Epub 2015 Nov 19. PMID: 26584622. PMC4691948.
Young SA, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, Aitken RJ, Baker MA, #Ikawa M. CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Int J Mol Sci. 2015 Oct 16;16(10):24732-50. doi: 10.3390/ijms161024732. PMID: 26501274. PMC4632774.
Miyata H, Satouh Y, Mashiko D, Muto M, Nozawa K, Shiba K, Fujihara Y, Isotani A, Inaba K, #Ikawa M. Sperm calcineurin inhibition prevents mouse fertility with implications for male contraceptive. Science. 2015 Oct 23;350(6259):442-5. doi: 10.1126/science.aad0836. Epub 2015 Oct 1. PMID: 26429887.
Yamaguchi M, Watanabe Y, Ohtani T, Uezumi A, Mikami N, Nakamura M, Sato T, Ikawa M, Hoshino M, Tsuchida K, Miyagoe-Suzuki Y, Tsujikawa K, Takeda S, Yamamoto H, #Fukada S. Calcitonin Receptor Signaling Inhibits Muscle Stem Cells from Escaping the Quiescent State and the Niche. Cell Rep. 2015 Oct 13;13(2):302-14. doi: 10.1016/j.celrep.2015.08.083. Epub 2015 Oct 1. PMID: 26440893.
Tokuhiro K, Satouh Y, Nozawa K, Isotani A, Fujihara Y, Hirashima Y, Matsumura H, Takumi K, Miyano T, Okabe M, Benham AM, #Ikawa M. Calreticulin is required for development of the cumulus oocyte complex and female fertility. Sci Rep. 2015 Sep 21;5:14254. doi: 10.1038/srep14254. PMID: 26388295. PMC4585710.
Satouh Y, Nozawa K, #Ikawa M. Sperm postacrosomal WW domain-binding protein is not required for mouse egg activation. Biol Reprod. 2015 Oct;93(4):94. doi: 10.1095/biolreprod.115.131441. Epub 2015 Sep 16. PMID: 26377222.
#Ono R, Ishii M, Fujihara Y, Kitazawa M, Usami T, Kaneko-Ishino T, Kanno J, Ikawa M, Ishino F. Double strand break repair by capture of retrotransposon sequences and reverse-transcribed spliced mRNA sequences in mouse zygotes. Sci Rep. 2015 Jul 28;5:12281. doi: 10.1038/srep12281. PMID: 26216318. PMC4516963.
Kidoya H, Naito H, Muramatsu F, Yamakawa D, Jia W, Ikawa M, Sonobe T, Tsuchimochi H, Shirai M, Adams RH, Fukamizu A, #Takakura N. APJ Regulates Parallel Alignment of Arteries and Veins in the Skin. Dev Cell. 2015 May 4;33(3):247-59. doi: 10.1016/j.devcel.2015.02.024. Epub 2015 Apr 23. PMID: 25920569.
Okuda H, Kiuchi H, Takao T, Miyagawa Y, Tsujimura A, Nonomura N, Miyata H, Okabe M, Ikawa M, Kawakami Y, Goshima N, Wada M, #Tanaka H. A novel transcriptional factor Nkapl is a germ cell-specific suppressor of Notch signaling and is indispensable for spermatogenesis. PLoS One. 2015 Apr 14;10(4):e0124293. doi: 10.1371/journal.pone.0124293. eCollection 2015. PMID: 25875095. PMC4397068.
#Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB. One-step generation of multiple transgenic mouse lines using an improved Pronuclear Injection-based Targeted Transgenesis (i-PITT). BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5. PMID: 25887549. PMC4404087.
Akincilar SC, Low KC, Liu CY, Yan TD, Oji A, Ikawa M, Li S, #Tergaonkar V. Quantitative assessment of telomerase components in cancer cell lines. FEBS Lett. 2015 Apr 13;589(9):974-84. doi: 10.1016/j.febslet.2015.02.035. Epub 2015 Mar 5. PMID: 25749370.
#Kimura T, Kaga Y, Sekita Y, Fujikawa K, Nakatani T, Odamoto M, Funaki S, Ikawa M, Abe K, Nakano T. Pluripotent stem cells derived from mouse primordial germ cells by small molecule compounds. Stem Cells. 2015 Jan;33(1):45-55. doi: 10.1002/stem.1838. PMID: 25186651.
#Honda A, Hirose M, Sankai T, Yasmin L, Yuzawa K, Honsho K, Izu H, Iguchi A, Ikawa M, Ogura A. Single-step generation of rabbits carrying a targeted allele of the tyrosinase gene using CRISPR/Cas9. Exp Anim. 2015;64(1):31-7. doi: 10.1538/expanim.14-0034. Epub 2014 Sep 8. PMID: 25195632. PMC4329513.
Kishimoto M, Matsuda T, Yanase S, Katsumi A, Suzuki N, Ikejiri M, Takagi A, Ikawa M, Kojima T, Kunishima S, Kiyoi H, Naoe T, Matsushita T, #Maruyama M. Rhof promotes murine marginal zone B cell development. Nagoya J Med Sci. 2014 Aug;76(3-4):293-305. PMID: 25741038. PMC4345693.
Qiu B, Shi X, Wong ET, Lim J, Bezzi M, Low D, Zhou Q, Akincilar SC, Lakshmanan M, Swa HL, Tham JM, Gunaratne J, Cheng KK, Hong W, Lam KS, Ikawa M, Guccione E, Xu A, Han W, #Tergaonkar V. NUCKS is a positive transcriptional regulator of insulin signaling. Cell Rep. 2014 Jun 26;7(6):1876-86. doi: 10.1016/j.celrep.2014.05.030. Epub 2014 Jun 12. PMID: 24931609.
Mizuno S, Dinh TT, Kato K, Mizuno-Iijima S, Tanimoto Y, Daitoku Y, Hoshino Y, Ikawa M, Takahashi S, #Sugiyama F, Yagami KI. Simple generation of albino C57BL/6J mice with G291T mutation in the tyrosinase gene by the CRISPR/Cas9 system. Mamm Genome. 2014 Aug;25(7-8):327-34. doi: 10.1007/s00335-014-9524-0. Epub 2014 May 31. PMID: 24879364.
#Takarada-Iemata M, Kezuka D, Takeichi T, Ikawa M, Hattori T, Kitao Y, Hori O. Deletion of N-myc downstream-regulated gene 2 attenuates reactive astrogliosis and inflammatory response in a mouse model of cortical stab injury. J Neurochem. 2014 Aug;130(3):374-87. doi: 10.1111/jnc.12729. Epub 2014 Apr 25. PMID: 24697507.
Fujihara Y, Okabe M, #Ikawa M. GPI-anchored protein complex, LY6K/TEX101, is required for sperm migration into the oviduct and male fertility in mice. Biol Reprod. 2014 Mar 20;90(3):60. doi: 10.1095/biolreprod.113.112888. Print 2014 Mar. PMID: 24501175.
Azuma K, Tsukui T, Ikeda K, Shiba S, Nakagawa K, Okano T, Urano T, Horie-Inoue K, Ouchi Y, Ikawa M, #Inoue S. Liver-specific gamma-glutamyl carboxylase-deficient mice display bleeding diathesis and short life span. PLoS One. 2014 Feb 10;9(2):e88643. doi: 10.1371/journal.pone.0088643. eCollection 2014. PMID: 24520408. PMC3919827.
Cheng J, Morisaki H, Sugimoto N, Dohi A, Shintani T, Kimura E, Toyama K, Ikawa M, Okabe M, Higuchi I, Matsuo S, Kawai Y, Hisatome I, Sugama T, Holmes EW, #Morisaki T. Effect of isolated AMP deaminase deficiency on skeletal muscle function. Mol Genet Metab Rep. 2014 Jan 16;1:51-59. doi: 10.1016/j.ymgmr.2013.12.004. eCollection 2014. PMID: 27896074. PMC5121310.
Mashiko D, Young SA, Muto M, Kato H, Nozawa K, Ogawa M, Noda T, Kim YJ, Satouh Y, Fujihara Y, #Ikawa M. Feasibility for a large scale mouse mutagenesis by injecting CRISPR/Cas plasmid into zygotes. Dev Growth Differ. 2014 Jan;56(1):122-9. doi: 10.1111/dgd.12113. Epub 2013 Dec 26. PMID: 24372541.
Mashiko D, Fujihara Y, Satouh Y, Miyata H, Isotani A, #Ikawa M. Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA. Sci Rep. 2013 Nov 27;3:3355. doi: 10.1038/srep03355. PMID: 24284873. PMC3842082.
Suzuki N, Kunishima S, Ikejiri M, Maruyama S, Sone M, Takagi A, Ikawa M, Okabe M, Kojima T, Saito H, Naoe T, #Matsushita T. Establishment of mouse model of MYH9 disorders: heterozygous R702C mutation provokes macrothrombocytopenia with leukocyte inclusion bodies, renal glomerulosclerosis and hearing disability. PLoS One. 2013 Aug 20;8(8):e71187. doi: 10.1371/journal.pone.0071187. eCollection 2013. PMID: 23976996. PMC3748045.
Tashiro-Yamaji J, Maeda S, Ikawa M, Okabe M, Kubota T, #Yoshida R. Macrophage MHC and T-cell receptors essential for rejection of allografted skin and lymphoma. Transplantation. 2013 Aug 15;96(3):251-7. doi: 10.1097/TP.0b013e3182985527. PMID: 23836286.
Ochiai N, Nishizuka M, Miyamoto T, Miyoshi I, Ikawa M, Osada S, #Imagawa M. Targeted disruption of fad24, a regulator of adipogenesis, causes pre- implantation embryonic lethality due to the growth defect at the blastocyst stage. Biochem Biophys Res Commun. 2013 Aug 23;438(2):301-5. doi: 10.1016/j.bbrc.2013.07.061. Epub 2013 Jul 22. PMID: 23886952.
Inoue N, Hamada D, Kamikubo H, Hirata K, Kataoka M, Yamamoto M, Ikawa M, Okabe M and #Hagihara Y. Molecular dissection of IZUMO1, a sperm protein essential for sperm-egg fusion. Development. 2013 Aug;140(15):3221-9. doi: 10.1242/dev.094854. Epub 2013 Jul 3. PMID: 23824580.
Hasuwa H, Ueda J, Ikawa M, #Okabe M. miR-200b and miR-429 function in mouse ovulation and are essential for female fertility. Science. 2013 Jul 5;341(6141):71-3. doi: 10.1126/science.1237999. Epub 2013 Jun 13. PMID: 23765281.
Naito Y, Yabuta N, Sato J, Ohno S, Sakata M, Kasama T, Ikawa M, #Nojima H. Recruitment of cyclin G2 to promyelocytic leukemia nuclear bodies promotes dephosphorylation of gammaH2AX following treatment with ionizing radiation. Cell Cycle. 2013 Jun 1;12(11):1773-84. doi: 10.4161/cc.24878. Epub 2013 May 8. PMID: 23656780. PMC3713135.
Fujihara Y, Tokuhiro K, Muro Y, Kondoh G, Araki Y, Ikawa M, #Okabe M. Expression of TEX101, regulated by ACE, is essential for the production of fertile mouse spermatozoa. Proc Natl Acad Sci U S A. 2013 May 14;110(20):8111-6. doi: 10.1073/pnas.1222166110. Epub 2013 Apr 30. PMID: 23633567. PMC3657793.
Nojima S, #Toyofuku T, Kamao H, Ishigami C, Kaneko J, Okuno T, Takamatsu H, Ito D, Kang S, Kimura T, Yoshida Y, Morimoto K, Maeda Y, Ogata A, Ikawa M, Morii E, Aozasa K, Takagi J, Takahashi M, *Kumanogoh A. A point mutation in Semaphorin 4A associates with defective endosomal sorting and causes retinal degeneration. Nat Commun. 2013;4:1406. doi: 10.1038/ncomms2420. PMID: 23360997. PMC3562463.
Yabuta N, Mukai S, Okamoto A, Okuzaki D, Suzuki H, Torigata K, Yoshida K, Okada N, Miura D, Ito A, Ikawa M, Okabe M, #Nojima H. N-terminal truncation of Lats1 causes abnormal cell growth control and chromosomal instability. J Cell Sci. 2013 Jan 15;126(Pt 2):508-20. doi: 10.1242/jcs.113431. Epub 2012 Dec 10. PMID: 23230145.
#Endo Y, Takahashi M, Iwaki D, Ishida Y, Nakazawa N, Kodama T, Matsuzaka T, Kanno K, Liu Y, Tsuchiya K, Kawamura I, Ikawa M, Waguri S, Wada I, Matsushita M, Schwaeble WJ, Fujita T. Mice deficient in ficolin, a lectin complement pathway recognition molecule, are susceptible to Streptococcus pneumoniae infection. J Immunol. 2012 Dec 15;189(12):5860-6. doi: 10.4049/jimmunol.1200836. Epub 2012 Nov 12. PMID: 23150716.
Wang J, Shiratori I, Uehori J, Ikawa M, #Arase H. Neutrophil infiltration during inflammation is regulated by PILRalpha via modulation of integrin activation. Nat Immunol. 2013 Jan;14(1):34-40. doi: 10.1038/ni.2456. Epub 2012 Nov 11. PMID: 23142774.
Cheng J, Morisaki H, Toyama K, Ikawa M, Okabe M, #Morisaki T. AMPD3-deficient mice exhibit increased erythrocyte ATP levels but anemia not improved due to PK deficiency. Genes Cells. 2012 Nov;17(11):913-22. doi: 10.1111/gtc.12006. Epub 2012 Oct 18. PMID: 23078545.
Fujihara Y, Satouh Y, Inoue N, Isotani A, Ikawa M, #Okabe M. SPACA1-deficient male mice are infertile with abnormally shaped sperm heads reminiscent of globozoospermia. Development. 2012 Oct;139(19):3583-9. doi: 10.1242/dev.081778. PMID: 22949614.
Satouh Y, Inoue N, Ikawa M, #Okabe M. Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1. J Cell Sci. 2012 Nov 1;125(Pt 21):4985-90. doi: 10.1242/jcs.100867. Epub 2012 Sep 3. PMID: 22946049.
Fujihara Y, Kaseda K, Inoue N, Ikawa M, #Okabe M. Production of mouse pups from germline transmission-failed knockout chimeras. Transgenic Res. 2013 Feb;22(1):195-200. doi: 10.1007/s11248-012-9635-x. Epub 2012 Jul 24. PMID: 22826106.
Masuda T, Itoh K, Higashitsuji H, Higashitsuji H, Nakazawa N, Sakurai T, Liu Y, Tokuchi H, Fujita T, Zhao Y, Nishiyama H, Tanaka T, Fukumoto M, Ikawa M, Okabe M, #Fujita J. Cold-inducible RNA-binding protein (Cirp) interacts with Dyrk1b/Mirk and promotes proliferation of immature male germ cells in mice. Proc Natl Acad Sci U S A. 2012 Jul 3;109(27):10885-90. doi: 10.1073/pnas.1121524109. Epub 2012 Jun 18. PMID: 22711815. PMC3390833.
Yamaguchi R, Fujihara Y, Ikawa M, #Okabe M. Mice expressing aberrant sperm-specific protein PMIS2 produce normal- looking but fertilization-incompetent spermatozoa. Mol Biol Cell. 2012 Jul;23(14):2671-9. doi: 10.1091/mbc.E11-12-1025. Epub 2012 May 23. PMID: 22621904. PMC3395656.
#Inoue N, Nishikawa T, Ikawa M, Okabe M. Tetraspanin-interacting protein IGSF8 is dispensable for mouse fertility. Fertil Steril. 2012 Aug;98(2):465-70. doi: 10.1016/j.fertnstert.2012.04.029. Epub 2012 May 19. PMID: 22609062.
#Tarutani M, Nakajima K, Uchida Y, Takaishi M, Goto-Inoue N, Ikawa M, Setou M, Kinoshita T, Elias PM, Sano S, Maeda Y. GPHR-dependent functions of the Golgi apparatus are essential for the formation of lamellar granules and the skin barrier. J Invest Dermatol. 2012 Aug;132(8):2019-25. doi: 10.1038/jid.2012.100. Epub 2012 May 10. PMID: 22572823.
Tokuhiro K, #Ikawa M, Benham AM, Okabe M. Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected]. Proc Natl Acad Sci U S A. 2012 Mar 6;109(10):3850-5. doi: 10.1073/pnas.1117963109. Epub 2012 Feb 22. PMID: 22357757. PMC3309714.
Toyama K, Morisaki H, Cheng J, Kawachi H, Shimizu F, Ikawa M, Okabe M, #Morisaki T. Proteinuria in AMPD2-deficient mice. Genes Cells. 2012 Jan;17(1):28-38. doi: 10.1111/j.1365-2443.2011.01568.x. PMID: 22212473.
Inoue N, Satouh Y, Ikawa M, *Okabe M, #Yanagimachi R. Acrosome-reacted mouse spermatozoa recovered from the perivitelline space can fertilize other eggs. Proc Natl Acad Sci U S A. 2011 Dec 13;108(50):20008-11. doi: 10.1073/pnas.1116965108. Epub 2011 Nov 14. PMID: 22084105. PMC3250175.
#Ito D, Yagi T, Ikawa M, Suzuki N. Characterization of inclusion bodies with cytoprotective properties formed by seipinopathy-linked mutant seipin. Hum Mol Genet. 2012 Feb 1;21(3):635-46. doi: 10.1093/hmg/ddr497. Epub 2011 Nov 1. PMID: 22045697.
#Mitsutake S, Zama K, Yokota H, Yoshida T, Tanaka M, Mitsui M, Ikawa M, Okabe M, Tanaka Y, Yamashita T, Takemoto H, Okazaki T, Watanabe K, Igarashi Y. Dynamic modification of sphingomyelin in lipid microdomains controls development of obesity, fatty liver, and type 2 diabetes. J Biol Chem. 2011 Aug 12;286(32):28544-55. doi: 10.1074/jbc.M111.255646. Epub 2011 Jun 13. PMID: 21669879. PMC3151096.
#Kanatsu-Shinohara M, Kato-Itoh M, Ikawa M, Takehashi M, Sanbo M, Morioka Y, Tanaka T, Morimoto H, Hirabayashi M, Shinohara T. Homologous recombination in rat germline stem cells. Biol Reprod. 2011 Jul;85(1):208-17. doi: 10.1095/biolreprod.111.090837. Epub 2011 Apr 6. PMID: 21471297.
Moriyama T, Nagai M, Oka M, Ikawa M, Okabe M, #Yoneda Y. Targeted disruption of one of the importin alpha family members leads to female functional incompetence in delivery. FEBS J. 2011 May;278(9):1561-72. doi: 10.1111/j.1742-4658.2011.08079.x. Epub 2011 Mar 22. PMID: 21371262.
Suga T, #Kimura E, Morioka Y, Ikawa M, Li S, Uchino K, Uchida Y, Yamashita S, Maeda Y, Chamberlain JS, Uchino M. Muscle fiber type-predominant promoter activity in lentiviral-mediated transgenic mouse. PLoS One. 2011 Mar 18;6(3):e16908. doi: 10.1371/journal.pone.0016908. PMID: 21445245. PMC3060803.
Isotani A, Hatayama H, Kaseda K, Ikawa M, #Okabe M. Formation of a thymus from rat ES cells in xenogeneic nude mouse<-->rat ES chimeras. Genes Cells. 2011 Apr;16(4):397-405. doi: 10.1111/j.1365-2443.2011.01495.x. Epub 2011 Mar 15. PMID: 21401810.
Nagai M, Moriyama T, Mehmood R, Tokuhiro K, Ikawa M, Okabe M, Tanaka H, #Yoneda Y. Mice lacking Ran binding protein 1 are viable and show male infertility. FEBS Lett. 2011 Mar 9;585(5):791-6. doi: 10.1016/j.febslet.2011.02.002. Epub 2011 Feb 26. PMID: 21310149.
Nakagawa Y, Takamatsu H, Okuno T, Kang S, Nojima S, Kimura T, Kataoka TR, Ikawa M, Toyofuku T, Katayama I, #Kumanogoh A. Identification of semaphorin 4B as a negative regulator of basophil- mediated immune responses. J Immunol. 2011 Mar 1;186(5):2881-8. doi: 10.4049/jimmunol.1003485. Epub 2011 Jan 26. PMID: 21270411.
Hayashi T, Nozaki Y, Nishizuka M, Ikawa M, Osada S, #Imagawa M. Factor for adipocyte differentiation 158 gene disruption prevents the body weight gain and insulin resistance induced by a high-fat diet. Biol Pharm Bull. 2011;34(8):1257-63. doi: 10.1248/bpb.34.1257. PMID: 21804215.
Kumasawa K, #Ikawa M, Kidoya H, Hasuwa H, Saito-Fujita T, Morioka Y, Takakura N, Kimura T, Okabe M. Pravastatin induces placental growth factor (PGF) and ameliorates preeclampsia in a mouse model. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1451-5. doi: 10.1073/pnas.1011293108. Epub 2010 Dec 27. PMID: 21187414. PMC3029692.
#Ikawa M, Tokuhiro K, Yamaguchi R, Benham AM, Tamura T, Wada I, Satouh Y, Inoue N, Okabe M. Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem. 2011 Feb 18;286(7):5639-46. doi: 10.1074/jbc.M110.140152. Epub 2010 Dec 3. PMID: 21131354. PMC3037677.
#Yano M, Watanabe K, Yamamoto T, Ikeda K, Senokuchi T, Lu M, Kadomatsu T, Tsukano H, Ikawa M, Okabe M, Yamaoka S, Okazaki T, Umehara H, Gotoh T, Song WJ, Node K, Taguchi R, Yamagata K, *Oike Y. Mitochondrial dysfunction and increased reactive oxygen species impair insulin secretion in sphingomyelin synthase 1-null mice. J Biol Chem. 2011 Feb 4;286(5):3992-4002. doi: 10.1074/jbc.M110.179176. Epub 2010 Nov 29. PMID: 21115496. PMC3030399.
Shibukawa Y, Yamazaki N, Kumasawa K, Daimon E, Tajiri M, Okada Y, Ikawa M, #Wada Y. Calponin 3 regulates actin cytoskeleton rearrangement in trophoblastic cell fusion. Mol Biol Cell. 2010 Nov 15;21(22):3973-84. doi: 10.1091/mbc.E10-03-0261. Epub 2010 Sep 22. PMID: 20861310. PMC2982094.
#Hosoyamada M, Takiue Y, Morisaki H, Cheng J, Ikawa M, Okabe M, Morisaki T, Ichida K, Hosoya T, Shibasaki T. Establishment and analysis of SLC22A12 (URAT1) knockout mouse. Nucleosides Nucleotides Nucleic Acids. 2010 Jun;29(4-6):314-20. doi: 10.1080/15257771003738634. PMID: 20544513.
Inoue N, Kasahara T, Ikawa M, #Okabe M. Identification and disruption of sperm-specific angiotensin converting enzyme-3 (ACE3) in mouse. PLoS One. 2010 Apr 22;5(4):e10301. doi: 10.1371/journal.pone.0010301. PMID: 20421979. PMC2858660.
Fujihara Y, Murakami M, Inoue N, Satouh Y, Kaseda K, Ikawa M, #Okabe M. Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse. J Cell Sci. 2010 May 1;123(Pt 9):1531-6. doi: 10.1242/jcs.067363. Epub 2010 Apr 7. PMID: 20375058.
Hasuwa H, Muro Y, Ikawa M, Kato N, Tsujimoto Y, #Okabe M. Transgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo. Exp Anim. 2010;59(1):105-7. doi: 10.1538/expanim.59.105. PMID: 20224175.
Ichise T, Adachi S, Ohishi M, Ikawa M, Okabe M, Iwamoto R, #Mekada E. Humanized gene replacement in mice reveals the contribution of cancer stroma-derived HB-EGF to tumor growth. Cell Struct Funct. 2010;35(1):3-13. doi: 10.1247/csf.09025. PMID: 20190463.
Morioka Y, Isotani A, Oshima RG, Okabe M, #Ikawa M. Placenta-specific gene activation and inactivation using integrase- defective lentiviral vectors with the Cre/LoxP system. Genesis. 2009 Dec;47(12):793-8. doi: 10.1002/dvg.20563. PMID: 19830817.
Saito A, Hino S, Murakami T, Kanemoto S, Kondo S, Saitoh M, Nishimura R, Yoneda T, Furuichi T, Ikegawa S, Ikawa M, Okabe M, #Imaizumi K. Regulation of endoplasmic reticulum stress response by a BBF2H7-mediated Sec23a pathway is essential for chondrogenesis. Nat Cell Biol. 2009 Oct;11(10):1197-204. doi: 10.1038/ncb1962. Epub 2009 Sep 20. PMID: 19767744.
Murakami T, Saito A, Hino S, Kondo S, Kanemoto S, Chihara K, Sekiya H, Tsumagari K, Ochiai K, Yoshinaga K, Saitoh M, Nishimura R, Yoneda T, Kou I, Furuichi T, Ikegawa S, Ikawa M, Okabe M, Wanaka A, #Imaizumi K. Signalling mediated by the endoplasmic reticulum stress transducer OASIS is involved in bone formation. Nat Cell Biol. 2009 Oct;11(10):1205-11. doi: 10.1038/ncb1963. Epub 2009 Sep 20. PMID: 19767743.
#Ohta H, Ohinata Y, Ikawa M, Morioka Y, Sakaide Y, Saitou M, Kanagawa O, Wakayama T. Male germline and embryonic stem cell lines from NOD mice: efficient derivation of GS cells from a nonpermissive strain for ES cell derivation. Biol Reprod. 2009 Dec;81(6):1147-53. doi: 10.1095/biolreprod.109.079368. Epub 2009 Sep 2. PMID: 19726737.
Kawasaki T, Saito K, Mitsui K, Ikawa M, Yamashita M, Taniguchi Y, Takeda S, Mitani K, #Sakai N. Introduction of a foreign gene into zebrafish and medaka cells using adenoviral vectors. Zebrafish. 2009 Sep;6(3):253-8. doi: 10.1089/zeb.2009.0596. PMID: 19715489.
Nimura K, *Ura K, Shiratori H, Ikawa M, Okabe M, Schwartz RJ, #Kaneda Y. A histone H3 lysine 36 trimethyltransferase links Nkx2-5 to Wolf- Hirschhorn syndrome. Nature. 2009 Jul 9;460(7252):287-91. doi: 10.1038/nature08086. Epub 2009 May 31. PMID: 19483677.
Yamaguchi R, Muro Y, Isotani A, Tokuhiro K, Takumi K, Adham I, Ikawa M, #Okabe M. Disruption of ADAM3 impairs the migration of sperm into oviduct in mouse. Biol Reprod. 2009 Jul;81(1):142-6. doi: 10.1095/biolreprod.108.074021. Epub 2009 Apr 1. PMID: 19339711.
Okada Y, Ueshin Y, Hasuwa H, Takumi K, Okabe M, #Ikawa M. Targeted gene modification in mouse ES cells using integrase-defective lentiviral vectors. Genesis. 2009 Apr;47(4):217-23. doi: 10.1002/dvg.20469. PMID: 19208434.
Iuchi Y, Okada F, Tsunoda S, Kibe N, Shirasawa N, Ikawa M, Okabe M, Ikeda Y, #Fujii J. Peroxiredoxin 4 knockout results in elevated spermatogenic cell death via oxidative stress. Biochem J. 2009 Apr 1;419(1):149-58. doi: 10.1042/BJ20081526. PMID: 19105792.
#Nagaoka K, Aoki F, Hayashi M, Muroi Y, Sakurai T, Itoh K, Ikawa M, Okabe M, Imakawa K, Sakai S. L-amino acid oxidase plays a crucial role in host defense in the mammary glands. FASEB J. 2009 Aug;23(8):2514-20. doi: 10.1096/fj.08-126466. Epub 2009 Mar 10. PMID: 19276171.
Nishizuka M, Kishimoto K, Kato A, Ikawa M, Okabe M, Sato R, Niida H, Nakanishi M, Osada S, #Imagawa M. Disruption of the novel gene fad104 causes rapid postnatal death and attenuation of cell proliferation, adhesion, spreading and migration. Exp Cell Res. 2009 Mar 10;315(5):809-19. doi: 10.1016/j.yexcr.2008.12.013. Epub 2008 Dec 29. PMID: 19138685.
Inoue N, Ikawa M, #Okabe M. Putative sperm fusion protein IZUMO and the role of N-glycosylation. Biochem Biophys Res Commun. 2008 Dec 19;377(3):910-4. doi: 10.1016/j.bbrc.2008.10.073. Epub 2008 Oct 24. PMID: 18952059.
Youn DY, Lee DH, Lim MH, Yoon JS, Lim JH, Jung SE, Yeum CE, Park CW, Youn HJ, Lee JS, Lee SB, Ikawa M, Okabe M, Tsujimoto Y, #Lee JH. Bis deficiency results in early lethality with metabolic deterioration and involution of spleen and thymus. Am J Physiol Endocrinol Metab. 2008 Dec;295(6):E1349-57. doi: 10.1152/ajpendo.90704.2008. Epub 2008 Oct 7. PMID: 18840758.
Yamaguchi R, Yamagata K, Hasuwa H, Inano E, Ikawa M, #Okabe M. Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse. Genes Cells. 2008 Aug;13(8):851-61. doi: 10.1111/j.1365-2443.2008.01210.x. PMID: 18782223.
Okiyoneda T, Niibori A, Harada K, Kohno T, Michalak M, Duszyk M, Wada I, Ikawa M, Shuto T, Suico MA, #Kai H. Role of calnexin in the ER quality control and productive folding of CFTR; differential effect of calnexin knockout on wild-type and DeltaF508 CFTR. Biochim Biophys Acta. 2008 Sep;1783(9):1585-94. doi: 10.1016/j.bbamcr.2008.04.002. Epub 2008 Apr 16. PMID: 18457676.
Kuramochi-Miyagawa S, Watanabe T, Gotoh K, Totoki Y, Toyoda A, Ikawa M, Asada N, Kojima K, Yamaguchi Y, Ijiri TW, Hata K, Li E, Matsuda Y, Kimura T, Okabe M, Sasaki Y, #Nakano T. DNA methylation of retrotransposon genes is regulated by Piwi family members MILI and MIWI2 in murine fetal testes. Genes Dev. 2008 Apr 1;22(7):908-17. doi: 10.1101/gad.1640708. PMID: 18381894. PMC2279202.
Ito T, Kimura Y, Uozumi Y, Takai M, Muraoka S, Matsuda T, Ueki K, Yoshiyama M, Ikawa M, Okabe M, Schaffer SW, Fujio Y, #Azuma J. Taurine depletion caused by knocking out the taurine transporter gene leads to cardiomyopathy with cardiac atrophy. J Mol Cell Cardiol. 2008 May;44(5):927-37. doi: 10.1016/j.yjmcc.2008.03.001. Epub 2008 Mar 18. PMID: 18407290.
Shinzawa K, Sumi H, Ikawa M, Matsuoka Y, Okabe M, Sakoda S, #Tsujimoto Y. Neuroaxonal dystrophy caused by group VIA phospholipase A2 deficiency in mice: a model of human neurodegenerative disease. J Neurosci. 2008 Feb 27;28(9):2212-20. doi: 10.1523/JNEUROSCI.4354-07.2008. PMID: 18305254. PMC6671850.
#Kanatsu-Shinohara M, Lee J, Inoue K, Ogonuki N, Miki H, Toyokuni S, Ikawa M, Nakamura T, Ogura A, Shinohara T. Pluripotency of a single spermatogonial stem cell in mice. Biol Reprod. 2008 Apr;78(4):681-7. doi: 10.1095/biolreprod.107.066068. Epub 2008 Jan 16. PMID: 18199882.
Kobayakawa K, Kobayakawa R, Matsumoto H, Oka Y, Imai T, Ikawa M, Okabe M, Ikeda T, Itohara S, Kikusui T, Mori K, #Sakano H. Innate versus learned odour processing in the mouse olfactory bulb. Nature. 2007 Nov 22;450(7169):503-8. doi: 10.1038/nature06281. Epub 2007 Nov 7. PMID: 17989651.
#Tsujikawa K, Yayama K, Hayashi T, Matsushita H, Yamaguchi T, Shigeno T, Ogitani Y, Hirayama M, Kato T, Fukada S, Takatori S, Kawasaki H, Okamoto H, Ikawa M, Okabe M, Yamamoto H. Hypertension and dysregulated proinflammatory cytokine production in receptor activity-modifying protein 1-deficient mice. Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16702-7. doi: 10.1073/pnas.0705974104. Epub 2007 Oct 8. PMID: 17923674. PMC2034234.
Ueda Y, Yamaguchi R, Ikawa M, Okabe M, Morii E, Maeda Y, #Kinoshita T. PGAP1 knock-out mice show otocephaly and male infertility. J Biol Chem. 2007 Oct 19;282(42):30373-80. doi: 10.1074/jbc.M705601200. Epub 2007 Aug 20. PMID: 17711852.
Umehara H, Kimura T, Ohtsuka S, Nakamura T, Kitajima K, Ikawa M, Okabe M, Niwa H, #Nakano T. Efficient derivation of embryonic stem cells by inhibition of glycogen synthase kinase-3. Stem Cells. 2007 Nov;25(11):2705-11. doi: 10.1634/stemcells.2007-0086. Epub 2007 Jul 19. PMID: 17641246.
Yabuta N, Okada N, Ito A, Hosomi T, Nishihara S, Sasayama Y, Fujimori A, Okuzaki D, Zhao H, Ikawa M, Okabe M, #Nojima H. Lats2 is an essential mitotic regulator required for the coordination of cell division. J Biol Chem. 2007 Jun 29;282(26):19259-71. doi: 10.1074/jbc.M608562200. Epub 2007 May 3. PMID: 17478426.
Okada Y, Ueshin Y, Isotani A, Saito-Fujita T, Nakashima H, Kimura K, Mizoguchi A, Oh-Hora M, Mori Y, Ogata M, Oshima RG, Okabe M, #Ikawa M. Complementation of placental defects and embryonic lethality by trophoblast-specific lentiviral gene transfer. Nat Biotechnol. 2007 Feb;25(2):233-7. doi: 10.1038/nbt1280. Epub 2007 Jan 14. PMID: 17220877.
Akazawa T, Ebihara T, Okuno M, Okuda Y, Shingai M, Tsujimura K, Takahashi T, Ikawa M, Okabe M, Inoue N, Okamoto-Tanaka M, Ishizaki H, Miyoshi J, Matsumoto M, #Seya T. Antitumor NK activation induced by the Toll-like receptor 3-TICAM-1 (TRIF) pathway in myeloid dendritic cells. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):252-7. doi: 10.1073/pnas.0605978104. Epub 2006 Dec 26. PMID: 17190817. PMC1765444.
Nakamura T, Arai Y, Umehara H, Masuhara M, Kimura T, Taniguchi H, Sekimoto T, Ikawa M, Yoneda Y, Okabe M, Tanaka S, Shiota K, #Nakano T. PGC7/Stella protects against DNA demethylation in early embryogenesis. Nat Cell Biol. 2007 Jan;9(1):64-71. doi: 10.1038/ncb1519. Epub 2006 Dec 3. PMID: 17143267.
Nagamori I, Yomogida K, Ikawa M, Okabe M, Yabuta N, #Nojima H. The testes-specific bZip type transcription factor Tisp40 plays a role in ER stress responses and chromatin packaging during spermiogenesis. Genes Cells. 2006 Oct;11(10):1161-71. doi: 10.1111/j.1365-2443.2006.01013.x. PMID: 16999736.
Yamaguchi R, Yamagata K, Ikawa M, Moss SB, #Okabe M. Aberrant distribution of ADAM3 in sperm from both angiotensin-converting enzyme (Ace)- and calmegin (Clgn)-deficient mice. Biol Reprod. 2006 Nov;75(5):760-6. doi: 10.1095/biolreprod.106.052977. Epub 2006 Jul 26. PMID: 16870943.
#Hori O, Miyazaki M, Tamatani T, Ozawa K, Takano K, Okabe M, Ikawa M, Hartmann E, Mai P, Stern DM, Kitao Y, Ogawa S. Deletion of SERP1/RAMP4, a component of the endoplasmic reticulum (ER) translocation sites, leads to ER stress. Mol Cell Biol. 2006 Jun;26(11):4257-67. doi: 10.1128/MCB.02055-05. PMID: 16705175. PMC1489087.
Takegahara N, Takamatsu H, Toyofuku T, Tsujimura T, Okuno T, Yukawa K, Mizui M, Yamamoto M, Prasad DV, Suzuki K, Ishii M, Terai K, Moriya M, Nakatsuji Y, Sakoda S, Sato S, Akira S, Takeda K, Inui M, Takai T, Ikawa M, Okabe M, #Kumanogoh A, #Kikutani H. Plexin-A1 and its interaction with DAP12 in immune responses and bone homeostasis. Nat Cell Biol. 2006 Jun;8(6):615-22. doi: 10.1038/ncb1416. Epub 2006 May 21. PMID: 16715077.
#Kanatsu-Shinohara M, Ikawa M, Takehashi M, Ogonuki N, Miki H, Inoue K, Kazuki Y, Lee J, Toyokuni S, Oshimura M, Ogura A, #Shinohara T. Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells. Proc Natl Acad Sci U S A. 2006 May 23;103(21):8018-23. doi: 10.1073/pnas.0601139103. Epub 2006 May 5. PMID: 16679411. PMC1472422.
Zhao H, Ito A, Kimura SH, Yabuta N, Sakai N, Ikawa M, Okabe M, Matsuzawa Y, Yamashita S, #Nojima H. RECS1 deficiency in mice induces susceptibility to cystic medial degeneration. Genes Genet Syst. 2006 Feb;81(1):41-50. doi: 10.1266/ggs.81.41. PMID: 16607040.
Kobayashi S, Isotani A, Mise N, Yamamoto M, Fujihara Y, Kaseda K, Nakanishi T, Ikawa M, Hamada H, Abe K, #Okabe M. Comparison of gene expression in male and female mouse blastocysts revealed imprinting of the X-linked gene, Rhox5/Pem, at preimplantation stages. Curr Biol. 2006 Jan 24;16(2):166-72. doi: 10.1016/j.cub.2005.11.071. PMID: 16431368.
Tanaka N, Ikawa M, Mata NL, #Verma IM. Choroidal neovascularization in transgenic mice expressing prokineticin 1: an animal model for age-related macular degeneration. Mol Ther. 2006 Mar;13(3):609-16. doi: 10.1016/j.ymthe.2005.08.024. Epub 2005 Nov 2. PMID: 16263331.
Tergaonkar V, Correa RG, Ikawa M, #Verma IM. Distinct roles of IkappaB proteins in regulating constitutive NF-kappaB activity. Nat Cell Biol. 2005 Sep;7(9):921-3. doi: 10.1038/ncb1296. PMID: 16136188.
#Uchida S, Sohara E, Rai T, Ikawa M, Okabe M, Sasaki S. Impaired urea accumulation in the inner medulla of mice lacking the urea transporter UT-A2. Mol Cell Biol. 2005 Aug;25(16):7357-63. doi: 10.1128/MCB.25.16.7357-7363.2005. PMID: 16055743. PMC1190230.
Inoue N, Ikawa M, Isotani A, #Okabe M. The immunoglobulin superfamily protein Izumo is required for sperm to fuse with eggs. Nature. 2005 Mar 10;434(7030):234-8. doi: 10.1038/nature03362. PMID: 15759005.
Hara-Chikuma M, Sohara E, Rai T, Ikawa M, Okabe M, Sasaki S, Uchida S, #Verkman AS. Progressive adipocyte hypertrophy in aquaporin-7-deficient mice: adipocyte glycerol permeability as a novel regulator of fat accumulation. J Biol Chem. 2005 Apr 22;280(16):15493-6. doi: 10.1074/jbc.C500028200. Epub 2005 Mar 3. PMID: 15746100.
Hanada R, Teranishi H, Pearson JT, Kurokawa M, Hosoda H, Fukushima N, Fukue Y, Serino R, Fujihara H, Ueta Y, Ikawa M, Okabe M, Murakami N, Shirai M, Yoshimatsu H, Kangawa K, #Kojima M. Neuromedin U has a novel anorexigenic effect independent of the leptin signaling pathway. Nat Med. 2004 Oct;10(10):1067-73. doi: 10.1038/nm1106. Epub 2004 Sep 26. PMID: 15448684.
Matsumura H, Hasuwa H, Inoue N, Ikawa M, #Okabe M. Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain. Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9. doi: 10.1016/j.bbrc.2004.06.139. PMID: 15358172.
Mori T, Yuxing Z, Takaki H, Takeuchi M, Iseki K, Hagino S, Kitanaka J, Takemura M, Misawa H, Ikawa M, Okabe M, #Wanaka A. The LIM homeobox gene, L3/Lhx8, is necessary for proper development of basal forebrain cholinergic neurons. Eur J Neurosci. 2004 Jun;19(12):3129-41. doi: 10.1111/j.0953-816X.2004.03415.x. PMID: 15217369.
Nakanishi T, Isotani A, Yamaguchi R, Ikawa M, Baba T, Suarez SS, #Okabe M. Selective passage through the uterotubal junction of sperm from a mixed population produced by chimeras of calmegin-knockout and wild-type male mice. Biol Reprod. 2004 Sep;71(3):959-65. doi: 10.1095/biolreprod.104.028647. Epub 2004 May 19. PMID: 15151931.
Shiomi N, Kito S, Oyama M, Matsunaga T, Harada YN, Ikawa M, Okabe M, #Shiomi T. Identification of the XPG region that causes the onset of Cockayne syndrome by using Xpg mutant mice generated by the cDNA-mediated knock-in method. Mol Cell Biol. 2004 May;24(9):3712-9. doi: 10.1128/MCB.24.9.3712-3719.2004. PMID: 15082767. PMC387744.
Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y, #Nakano T. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development. 2004 Feb;131(4):839-49. doi: 10.1242/dev.00973. Epub 2004 Jan 21. PMID: 14736746.
Tergaonkar V, Bottero V, Ikawa M, Li Q, #Verma IM. IkappaB kinase-independent IkappaBalpha degradation pathway: functional NF-kappaB activity and implications for cancer therapy. Mol Cell Biol. 2003 Nov;23(22):8070-83. doi: 10.1128/MCB.23.22.8070-8083.2003. PMID: 14585967. PMC262380.
Ikawa M, Tanaka N, Kao WW, #Verma IM. Generation of transgenic mice using lentiviral vectors: a novel preclinical assessment of lentiviral vectors for gene therapy. Mol Ther. 2003 Oct;8(4):666-73. doi: 10.1016/s1525-0016(03)00240-5. PMID: 14529840.
Inoue N, Ikawa M, Nakanishi T, Matsumoto M, Nomura M, Seya T, #Okabe M. Disruption of mouse CD46 causes an accelerated spontaneous acrosome reaction in sperm. Mol Cell Biol. 2003 Apr;23(7):2614-22. doi: 10.1128/MCB.23.7.2614-2622.2003. PMID: 12640142. PMC150730.
Kimura T, Ito C, Watanabe S, Takahashi T, Ikawa M, Yomogida K, Fujita Y, Ikeuchi M, Asada N, Matsumiya K, Okuyama A, Okabe M, Toshimori K, #Nakano T. Mouse germ cell-less as an essential component for nuclear integrity. Mol Cell Biol. 2003 Feb;23(4):1304-15. doi: 10.1128/MCB.23.4.1304-1315.2003. PMID: 12556490. PMC141152.
Tiscornia G, Singer O, Ikawa M, #Verma IM. A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA. Proc Natl Acad Sci U S A. 2003 Feb 18;100(4):1844-8. doi: 10.1073/pnas.0437912100. Epub 2003 Jan 27. PMID: 12552109. PMC149921.
Nakanishi T, Kuroiwa A, Yamada S, Isotani A, Yamashita A, Tairaka A, Hayashi T, Takagi T, Ikawa M, Matsuda Y, #Okabe M. FISH analysis of 142 EGFP transgene integration sites into the mouse genome. Genomics. 2002 Dec;80(6):564-74. doi: 10.1006/geno.2002.7008. PMID: 12504848.
Yamagata K, Nakanishi T, Ikawa M, Yamaguchi R, Moss SB, #Okabe M. Sperm from the calmegin-deficient mouse have normal abilities for binding and fusion to the egg plasma membrane. Dev Biol. 2002 Oct 15;250(2):348-57. PMID: 12376108.
Baba D, Kashiwabara S, Honda A, Yamagata K, Wu Q, Ikawa M, Okabe M, #Baba T. Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg. J Biol Chem. 2002 Aug 16;277(33):30310-4. doi: 10.1074/jbc.M204596200. Epub 2002 Jun 13. PMID: 12065596.
Ikawa M, Tergaonkar V, Ogura A, Ogonuki N, Inoue K, #Verma IM. Restoration of spermatogenesis by lentiviral gene transfer: offspring from infertile mice. Proc Natl Acad Sci U S A. 2002 May 28;99(11):7524-9. doi: 10.1073/pnas.072207299. PMID: 12032316. PMC124271.
Pfeifer A, Ikawa M, Dayn Y, #Verma IM. Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci U S A. 2002 Feb 19;99(4):2140-5. doi: 10.1073/pnas.251682798. PMID: 11854510. PMC122332.
Ikawa M, Nakanishi T, Yamada S, Wada I, Kominami K, Tanaka H, Nozaki M, Nishimune Y, #Okabe M. Calmegin is required for fertilin alpha/beta heterodimerization and sperm fertility. Dev Biol. 2001 Dec 1;240(1):254-61. doi: 10.1006/dbio.2001.0462. PMID: 11784061.
#Kitao Y, Ozawa K, Miyazaki M, Tamatani M, Kobayashi T, Yanagi H, Okabe M, Ikawa M, Yamashima T, Stern DM, Hori O, Ogawa S. Expression of the endoplasmic reticulum molecular chaperone (ORP150) rescues hippocampal neurons from glutamate toxicity. J Clin Invest. 2001 Nov;108(10):1439-50. doi: 10.1172/JCI12978. PMID: 11714735. PMC209417.
Nakanishi T, Ikawa M, Yamada S, Toshimori K, #Okabe M. Alkalinization of acrosome measured by GFP as a pH indicator and its relation to sperm capacitation. Dev Biol. 2001 Sep 1;237(1):222-31. doi: 10.1006/dbio.2001.0353. PMID: 11518518.
Horie K, Kuroiwa A, Ikawa M, Okabe M, Kondoh G, Matsuda Y, #Takeda J. Efficient chromosomal transposition of a Tc1/mariner- like transposon Sleeping Beauty in mice. Proc Natl Acad Sci U S A. 2001 Jul 31;98(16):9191-6. doi: 10.1073/pnas.161071798. PMID: 11481482. PMC55396.
Kimura SH, Ikawa M, Ito A, Okabe M, #Nojima H. Cyclin G1 is involved in G2/M arrest in response to DNA damage and in growth control after damage recovery. Oncogene. 2001 May 31;20(25):3290-300. doi: 10.1038/sj.onc.1204270. PMID: 11423978.
#Miyagawa S, Yamada M, Matsunami K, Koresawa Y, Ikawa M, Okabe M, Shirakura R. A synthetic dDAF (CD55) gene based on optimal codon usage for transgenic animals. J Biochem. 2001 May;129(5):795-801. doi: 10.1093/oxfordjournals.jbchem.a002922. PMID: 11328604.
Yamada M, #Miyagawa S, Matsunami K, Koresawa Y, Kurihara T, Ikawa M, Okabe M, Shirakura R. A codon exchange DAF is a powerful material for xenografting. Transplant Proc. 2001 Feb-Mar;33(1-2):744-5. doi: 10.1016/s0041-1345(00)02233-8. PMID: 11267049.
Koresawa Y, #Miyagawa S, Ikawa M, Matsunami K, Yamada M, Okabe M, Shirakura R. A new Cre recombinase gene based on optimal codon usage in mammals: a powerful material for organ-specific gene targeting. Transplant Proc. 2000 Nov;32(7):2516-7. doi: 10.1016/s0041-1345(00)01773-5. PMID: 11120274.
Shi W, Kumanogoh A, Watanabe C, Uchida J, Wang X, Yasui T, Yukawa K, Ikawa M, Okabe M, Parnes JR, Yoshida K, #Kikutani H. The class IV semaphorin CD100 plays nonredundant roles in the immune system: defective B and T cell activation in CD100-deficient mice. Immunity. 2000 Nov;13(5):633-42. doi: 10.1016/s1074-7613(00)00063-7. PMID: 11114376.
Hashimoto H, Shintani N, Nishino A, Okabe M, Ikawa M, Matsuyama S, Itoh K, Yamamoto K, Tomimoto S, Fujita T, Hagihara N, Mori W, Koyama Y, Matsuda T, Nagata S, #Baba A. Mice with markedly reduced PACAP (PAC(1)) receptor expression by targeted deletion of the signal peptide. J Neurochem. 2000 Nov;75(5):1810-7. doi: 10.1046/j.1471-4159.2000.0751810.x. PMID: 11032869.
Keng VW, Yagi H, Ikawa M, Nagano T, Myint Z, Yamada K, Tanaka T, Sato A, Muramatsu I, Okabe M, Sato M, #Noguchi T. Homeobox gene Hex is essential for onset of mouse embryonic liver development and differentiation of the monocyte lineage. Biochem Biophys Res Commun. 2000 Oct 5;276(3):1155-61. doi: 10.1006/bbrc.2000.3548. PMID: 11027604.
Koresawa Y, #Miyagawa S, Ikawa M, Matsunami K, Yamada M, Shirakura R, Okabe M. Synthesis of a new Cre recombinase gene based on optimal codon usage for mammalian systems. J Biochem. 2000 Mar;127(3):367-72. doi: 10.1093/oxfordjournals.jbchem.a022617. PMID: 10731707.
Kawakami N, Sakane N, Nishizawa F, Iwao M, Fukada SI, Tsujikawa K, Kohama Y, Ikawa M, Okabe M, #Yamamoto H. Green fluorescent protein-transgenic mice: immune functions and their application to studies of lymphocyte development. Immunol Lett. 1999 Dec 1;70(3):165-71. doi: 10.1016/s0165-2478(99)00152-2. PMID: 10656669.
Kawakami N, Nishizawa F, Sakane N, Iwao M, Tsujikawa K, Ikawa M, Okabe M, #Yamamoto H. Roles of integrins and CD44 on the adhesion and migration of fetal liver cells to the fetal thymus. J Immunol. 1999 Sep 15;163(6):3211-6. PMID: 10477589.
Ono K, Takii T, Onozaki K, Ikawa M, Okabe M, #Sawada M. Migration of exogenous immature hematopoietic cells into adult mouse brain parenchyma under GFP-expressing bone marrow chimera. Biochem Biophys Res Commun. 1999 Sep 7;262(3):610-4. doi: 10.1006/bbrc.1999.1223. PMID: 10471372.
Kato M, Yamanouchi K, Ikawa M, Okabe M, Naito K, #Tojo H. Efficient selection of transgenic mouse embryos using EGFP as a marker gene. Mol Reprod Dev. 1999 Sep;54(1):43-8. doi: 10.1002/(SICI)1098-2795(199909)54:1<43::AID-MRD6>3.0.CO;2-N. PMID: 10423296.
#Hamada Y, Kadokawa Y, Okabe M, Ikawa M, Coleman JR, Tsujimoto Y. Mutation in ankyrin repeats of the mouse Notch2 gene induces early embryonic lethality. Development. 1999 Aug;126(15):3415-24. doi: 10.1242/dev.126.15.3415. PMID: 10393120.
Nakanishi T, Ikawa M, Yamada S, Parvinen M, Baba T, Nishimune Y, #Okabe M. Real-time observation of acrosomal dispersal from mouse sperm using GFP as a marker protein. FEBS Lett. 1999 Apr 23;449(2-3):277-83. doi: 10.1016/s0014-5793(99)00433-0. PMID: 10338148.
Harada YN, Shiomi N, Koike M, Ikawa M, Okabe M, Hirota S, Kitamura Y, Kitagawa M, Matsunaga T, Nikaido O, #Shiomi T. Postnatal growth failure, short life span, and early onset of cellular senescence and subsequent immortalization in mice lacking the xeroderma pigmentosum group G gene. Mol Cell Biol. 1999 Mar;19(3):2366-72. doi: 10.1128/MCB.19.3.2366. PMID: 10022922. PMC84028.
#Imai E, Akagi Y, Isaka Y, Ikawa M, Takenaka M, Hori M, Okabe M. Glowing podocytes in living mouse: transgenic mouse carrying a podocyte- specific promoter. Exp Nephrol. 1999 Jan-Feb;7(1):63-6. doi: 10.1159/000020586. PMID: 9892816.
Hadjantonakis AK, Gertsenstein M, Ikawa M, Okabe M, #Nagy A. Generating green fluorescent mice by germline transmission of green fluorescent ES cells. Mech Dev. 1998 Aug;76(1-2):79-90. doi: 10.1016/s0925-4773(98)00093-8. PMID: 9867352.
Hadjantonakis A, Gertsenstein M, Ikawa M, Okabe M, #Nagy A. Non-invasive sexing of preimplantation stage mammalian embryos. Nat Genet. 1998 Jul;19(3):220-2. doi: 10.1038/893. PMID: 9662390.
Mikata S, #Miyagawa S, Yoshitatsu M, Ikawa M, Okabe M, Matsuda H, Shirakura R. Prevention of hyperacute rejection by phosphatidylinositol-anchored mini- complement receptor type 1. Transpl Immunol. 1998 Jun;6(2):107-10. doi: 10.1016/s0966-3274(98)80025-7. PMID: 9777699.
Matsuki M, Yamashita F, Ishida-Yamamoto A, Yamada K, Kinoshita C, Fushiki S, Ueda E, Morishima Y, Tabata K, Yasuno H, Hashida M, Iizuka H, Ikawa M, Okabe M, Kondoh G, Kinoshita T, Takeda J, #Yamanishi K. Defective stratum corneum and early neonatal death in mice lacking the gene for transglutaminase 1 (keratinocyte transglutaminase). Proc Natl Acad Sci U S A. 1998 Feb 3;95(3):1044-9. doi: 10.1073/pnas.95.3.1044. PMID: 9448282. PMC18665.
Tanaka H, Ikawa M, Tsuchida J, Nozaki M, Suzuki M, Fujiwara T, Okabe M, #Nishimune Y. Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone. Gene. 1997 Dec 19;204(1-2):159-63. doi: 10.1016/s0378-1119(97)00537-4. PMID: 9434179.
Tarutani M, Itami S, Okabe M, Ikawa M, Tezuka T, Yoshikawa K, Kinoshita T, #Takeda J. Tissue-specific knockout of the mouse Pig-a gene reveals important roles for GPI-anchored proteins in skin development. Proc Natl Acad Sci U S A. 1997 Jul 8;94(14):7400-5. doi: 10.1073/pnas.94.14.7400. PMID: 9207103. PMC23833.
Ikawa M, Wada I, Kominami K, Watanabe D, Toshimori K, Nishimune Y, #Okabe M. The putative chaperone calmegin is required for sperm fertility. Nature. 1997 Jun 5;387(6633):607-11. doi: 10.1038/42484. PMID: 9177349.
#Okabe M, Ikawa M, Kominami K, Nakanishi T, Nishimune Y. 'Green mice' as a source of ubiquitous green cells. FEBS Lett. 1997 May 5;407(3):313-9. doi: 10.1016/s0014-5793(97)00313-x. PMID: 9175875.
#Miyagawa S, Mikata S, Tanaka H, Ikawa M, Kominami K, Seya T, Nishimune Y, Shirakura R, Okabe M. The regulation of membrane cofactor protein (CD46) expression by the 3' untranslated region in transgenic mice. Biochem Biophys Res Commun. 1997 Apr 28;233(3):829-33. doi: 10.1006/bbrc.1997.6556. PMID: 9168942.
Akagi Y, Isaka Y, Akagi A, Ikawa M, Takenaka M, Moriyama T, Yamauchi A, Horio M, Ueda N, Okabe M, #Imai E. Transcriptional activation of a hybrid promoter composed of cytomegalovirus enhancer and beta-actin/beta-globin gene in glomerular epithelial cells in vivo. Kidney Int. 1997 Apr;51(4):1265-9. doi: 10.1038/ki.1997.172. PMID: 9083295.
#Miyagawa S, Ikawa M, Kominami K, Tanaka H, Mikata S, Matsuda H, Seya T, Shirakura R, Okabe M. The regulation of membrane cofactor protein (CD46) expression in transgenic mice: the importance of the first 125 BP of the 3' untranslated region. Transplant Proc. 1997 Feb-Mar;29(1-2):941-2. doi: 10.1016/s0041-1345(96)00266-7. PMID: 9123597.
Kawagoe K, Kitamura D, Okabe M, Taniuchi I, Ikawa M, Watanabe T, Kinoshita T, #Takeda J. Glycosylphosphatidylinositol-anchor-deficient mice: implications for clonal dominance of mutant cells in paroxysmal nocturnal hemoglobinuria. Blood. 1996 May 1;87(9):3600-6. PMID: 8611683.
#Miyagawa S, Ikawa M, Kominami K, Yoshimura Y, Tanaka H, Mikata S, Shirakura R, Matsuda H, Yanagi Y, Seya T, Okabe M. Difference of expression levels between gene technological product delta CYT-MCP(CD46) and intact MCP(CD46) in transgenic mice. Transplant Proc. 1996 Apr;28(2):585-6. PMID: 8623285.
#Miyagawa S, Mikata M, Matsuda H, Ikawa M, Okabe M, Nagasawa S, Matsumoto M, Seya T, Shirakura R. In vitro and in vivo studies to prevent hyperacute rejection. Transplant Proc. 1996 Apr;28(2):1031-3. PMID: 8623217.
Ikawa M, Kominami K, Yoshimura Y, Tanaka K, Nishimune Y, #Okabe M. A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP). FEBS Lett. 1995 Nov 13;375(1-2):125-8. doi: 10.1016/0014-5793(95)01162-8. PMID: 7498460.
Ikawa M, Kominami K, Yoshimura Y, Tanaka K, Nishimune Y, #Okabe M. Green fluorescent protein as a marker in transgenic mice. Dev Growth Differ. 1995 Aug;37(4):455-459. doi: 10.1046/j.1440-169X.1995.t01-2-00012.x. PMID: 37281722.
Ikawa M, Ying X, Tanaka K, Ohashi K, Saji F, #Okabe M. Improvement of fusing ability of human sperm to zona-free hamster eggs by conditioned media. Biol Pharm Bull. 1995 Jan;18(1):5-8. doi: 10.1248/bpb.18.5. PMID: 7735250.
Okabe M, Ying X, Nagira M, Ikawa M, Kohama Y, Mimura T, #Tanaka K. Homology of an acrosome-reacted sperm-specific antigen to CD46. J Pharmacobiodyn. 1992 Aug;15(8):455-9. doi: 10.1248/bpb1978.15.455. PMID: 1479546.
2）査読付き欧文国際雑誌に掲載された総説論文等
Hiraoka T, Hirota Y, #Ikawa M. Toward Implantation-Assisting Technologies: Lessons From In Vivo and Ex Vivo Models. Reprod Med Biol. 2026 Mar 16;25(1):e70042. doi: 10.1002/rmb2.70042. eCollection 2026 Jan-Dec. PMID: 41937975. PMC13045413.
#Miyata H, *Shimada K, Kaneda Y, #Ikawa M. Development of functional spermatozoa in mammalian spermiogenesis. Development. 2024 Jul 15;151(14):dev202838. doi: 10.1242/dev.202838. PMID: 39036999.
#Ozawa M, Emori C, #Ikawa M. Gene Targeting in Mouse Embryonic Stem Cells via CRISPR/Cas9 Ribonucleoprotein (RNP)-Mediated Genome Editing. Methods Mol Biol. 2023;2637:87-97. doi: 10.1007/978-1-0716-3016-7_7. PMID: 36773140.
#Lu Y, #Ikawa M. Eukaryotic fertilization and gamete fusion at a glance. J Cell Sci. 2022 Nov 15;135(22). pii: 284365. doi: 10.1242/jcs.260296. Epub 2022 Nov 23. PMID: 36416181. PMC10658792.
#Ozawa M, *Emori C, #Ikawa M. CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models. J Vis Exp. 2022 Aug 24;(186). doi: 10.3791/64385. PMID: 36094255.
#Kiyozumi D, #Ikawa M. Proteolysis in Reproduction: Lessons From Gene-Modified Organism Studies. Front Endocrinol (Lausanne). 2022 May 4;13:876370. doi: 10.3389/fendo.2022.876370. eCollection 2022. PMID: 35600599. PMC9114714.
#Miyagawa S, Maeda A, Toyama C, Kogata S, Okamatsu C, Yamamoto R, Masahata K, Kamiyama M, Eguchi H, Watanabe M, Nagashima H, Ikawa M, Matsunami K, Okuyama H. Aspects of the Complement System in New Era of Xenotransplantation. Front Immunol. 2022 Apr 14;13:860165. doi: 10.3389/fimmu.2022.860165. eCollection 2022. PMID: 35493484. PMC9046582.
El Hussien MA, Tsai CY, Satouh Y, Motooka D, Okuzaki D, Ikawa M, #Kikutani H, #Sakakibara S. Corrigendum to: Multiple tolerance checkpoints restrain affinity maturation of B cells expressing the germline precursor of a lupus patient-derived anti-dsDNA antibody in knock-in mice. Int Immunol. 2022 Mar 25;34(4):225. doi: 10.1093/intimm/dxac007. PMID: 35348696.
Qu Y, Chen Q, Guo S, Ma C, Lu Y, Shi J, Liu S, Zhou T, Noda T, Qian J, Zhang L, Zhu X, Lei X, Cao Y, Li W, Li W, Plachta N, Matzuk MM, Ikawa M, #Duan E, #Zhang Y, #Wang H. Cooperation-based sperm clusters mediate sperm oviduct entry and fertilization. Protein Cell. 2021 Oct;12(10):810-817. doi: 10.1007/s13238-021-00825-y. Epub 2021 Mar 1. PMID: 33649997. PMC8464547.
#Kim CR, Noda T, Okada Y, #Ikawa M, #Baek SH. Protocol for isolation of spermatids from mouse testes. STAR Protoc. 2021 Jan 8;2(1):100254. doi: 10.1016/j.xpro.2020.100254. eCollection 2021 Mar 19. PMID: 33490974. PMC7809437.
#Miyata H, Abbasi F, Visconti PE, #Ikawa M. CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in micedagger. Biol Reprod. 2020 Oct 29;103(5):912-914. doi: 10.1093/biolre/ioaa161. PMID: 32901808. PMC7609874.
#Miyata H, Morohoshi A, #Ikawa M. Analysis of the sperm flagellar axoneme using gene-modified mice. Exp Anim. 2020 Nov 12;69(4):374-381. doi: 10.1538/expanim.20-0064. Epub 2020 Jun 18. PMID: 32554934. PMC7677079.
Noda T, #Ikawa M. Physiological function of seminal vesicle secretions on male fecundity. Reprod Med Biol. 2019 Jun 17;18(3):241-246. doi: 10.1002/rmb2.12282. eCollection 2019 Jul. PMID: 31312102. PMC6613004.
#Satouh Y, #Ikawa M. New Insights into the Molecular Events of Mammalian Fertilization. Trends Biochem Sci. 2018 Oct;43(10):818-828. doi: 10.1016/j.tibs.2018.08.006. Epub 2018 Aug 28. PMID: 30170889. PMC6162164.
Fujihara Y, Miyata H, #Ikawa M. Factors controlling sperm migration through the oviduct revealed by gene- modified mouse models. Exp Anim. 2018 May 10;67(2):91-104. doi: 10.1538/expanim.17-0153. Epub 2018 Jan 22. PMID: 29353867. PMC5955741.
Abbasi F, Miyata H, #Ikawa M. Revolutionizing male fertility factor research in mice by using the genome editing tool CRISPR/Cas9. Reprod Med Biol. 2017 Oct 14;17(1):3-10. doi: 10.1002/rmb2.12067. eCollection 2018 Jan. PMID: 29371815. PMC5768971.
Tobita T, Kiyozumi D, #Ikawa M. Placenta-specific gene manipulation using lentiviral vector and its application. Placenta. 2017 Nov;59 Suppl 1:S37-S43. doi: 10.1016/j.placenta.2017.09.012. Epub 2017 Sep 28. PMID: 28988726.
Noda T, Oji A, #Ikawa M. Genome Editing in Mouse Zygotes and Embryonic Stem Cells by Introducing SgRNA/Cas9 Expressing Plasmids. Methods Mol Biol. 2017;1630:67-80. doi: 10.1007/978-1-4939-7128-2_6. PMID: 28643250.
Fujihara Y, #Ikawa M. GPI-AP release in cellular, developmental, and reproductive biology. J Lipid Res. 2016 Apr;57(4):538-45. doi: 10.1194/jlr.R063032. Epub 2015 Nov 22. PMID: 26593072. PMC4808780.
Young SA, Aitken RJ, #Ikawa M. Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models. Asian J Androl. 2015 Jul-Aug;17(4):623-7. doi: 10.4103/1008-682X.153851. PMID: 25994645. PMC4492054.
Fujihara Y, #Ikawa M. CRISPR/Cas9-based genome editing in mice by single plasmid injection. Methods Enzymol. 2014;546:319-36. doi: 10.1016/B978-0-12-801185-0.00015-5. PMID: 25398347.
Inoue N, Ikawa M, #Okabe M. The mechanism of sperm-egg interaction and the involvement of IZUMO1 in fusion. Asian J Androl. 2011 Jan;13(1):81-7. doi: 10.1038/aja.2010.70. Epub 2010 Nov 8. PMID: 21057513. PMC3739395.
Ikawa M, Inoue N, Benham AM, #Okabe M. Fertilization: a sperm's journey to and interaction with the oocyte. J Clin Invest. 2010 Apr;120(4):984-94. doi: 10.1172/JCI41585. Epub 2010 Apr 1. PMID: 20364096. PMC2846064.
Ikawa M, Inoue N, #Okabe M. Mechanisms of sperm-egg interactions emerging from gene-manipulated animals. Int J Dev Biol. 2008;52(5-6):657-64. doi: 10.1387/ijdb.072529mi. PMID: 18649279.
Sato T, Kurokawa M, Nakashima Y, Ida T, Takahashi T, Fukue Y, Ikawa M, Okabe M, Kangawa K, #Kojima M. Ghrelin deficiency does not influence feeding performance. Regul Pept. 2008 Jan 10;145(1-3):7-11. doi: 10.1016/j.regpep.2007.09.010. Epub 2007 Sep 18. PMID: 17913260.
Inoue N, Yamaguchi R, Ikawa M, #Okabe M. Sperm-egg interaction and gene manipulated animals. Soc Reprod Fertil Suppl. 2007;65:363-71. PMID: 17644977.
Singer O, Tiscornia G, Ikawa M, #Verma IM. Rapid generation of knockdown transgenic mice by silencing lentiviral vectors. Nat Protoc. 2006;1(1):286-92. doi: 10.1038/nprot.2006.44. PMID: 17406246.
Ikawa M, Yamada S, Nakanishi T, #Okabe M. Green fluorescent protein (GFP) as a vital marker in mammals. Curr Top Dev Biol. 1999;44:1-20. doi: 10.1016/s0070-2153(08)60465-2. PMID: 9891875.
Ikawa M, Yamada S, Nakanishi T, #Okabe M. 'Green mice' and their potential usage in biological research. FEBS Lett. 1998 Jun 23;430(1-2):83-7. doi: 10.1016/s0014-5793(98)00593-6. PMID: 9678599.
Okabe M, Ikawa M, #Ashkenas J. Male infertility and the genetics of spermatogenesis. Am J Hum Genet. 1998 Jun;62(6):1274-81. doi: 10.1086/301895. PMID: 9644029. PMC1377172.